Stimuli of Sensory-Motor Nerves Terminate Arterial Contractile Effects of Endothelin-1 by CGRP and Dissociation of ET-1/ETA-Receptor Complexes

Endothelin-1 (ET-1), a long-acting paracrine mediator, is implicated in cardiovascular diseases but clinical trials with ET-receptor antagonists were not successful in some areas. We tested whether the quasi-irreversible receptor-binding of ET-1 (i) limits reversing effects of the antagonists and (ii) can be selectively dissociated by an endogenous counterbalancing mechanism.-receptor complexes.-receptors by ET-1 (i) occur at an antagonist-insensitive site of the receptor and (ii) are selectively terminated by endogenously released CGRP. Hence, natural stimuli of sensory-motor nerves that stimulate release of endogenous CGRP can be considered for therapy of diseases involving ET-1

Call to Action: SARS-CoV-2 and CerebrovAscular DisordErs (CASCADE)

Background and purpose: The novel severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2), now named coronavirus disease 2019 (COVID-19), may change the risk of stroke through an enhanced systemic inflammatory response, hypercoagulable state, and endothelial damage in the cerebrovascular system. Moreover, due to the current pandemic, some countries have prioritized health resources towards COVID-19 management, making it more challenging to appropriately care for other potentially disabling and fatal diseases such as stroke. The aim of this study is to identify and describe changes in stroke epidemiological trends before, during, and after the COVID-19 pandemic. Methods: This is an international, multicenter, hospital-based study on stroke incidence and outcomes during the COVID-19 pandemic. We will describe patterns in stroke management, stroke hospitalization rate, and stroke severity, subtype (ischemic/hemorrhagic), and outcomes (including in-hospital mortality) in 2020 during COVID-19 pandemic, comparing them with the corresponding data from 2018 and 2019, and subsequently 2021. We will also use an interrupted time series (ITS) analysis to assess the change in stroke hospitalization rates before, during, and after COVID-19, in each participating center. Conclusion: The proposed study will potentially enable us to better understand the changes in stroke care protocols, differential hospitalization rate, and severity of stroke, as it pertains to the COVID-19 pandemic. Ultimately, this will help guide clinical-based policies surrounding COVID-19 and other similar global pandemics to ensure that management of cerebrovascular comorbidity is appropriately prioritized during the global crisis. It will also guide public health guidelines for at-risk populations to reduce risks of complications from such comorbidities. © 202

Quercetin Loaded Silica and Gold - Coated Silica Nanoparticles: Characterization, Evaluation and Comparison of Their in vitro Characteristics

Herein, silica nanoparticles (NPs) and gold-silica NPs were loaded with the anti-cancer agent quercetin (QC) to produce silica NPs-QC and gold coated silica NPs-QC, respectively. The nanosystems were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS) and Fourier transform infrared (FTIR). Drug encapsulation efficiency (EE), loading capacity (LC) and release rate were measured using UV spectrophotometer. The drug was encapsulated in silica NPs in a high percentage (71%) and reduced by about 16% after gold coating. The mean particle size increased after coating and QC loading with a polydispersity index (PDI) between (∼ 0.2 - 0.6) and negative zeta potential (-13 to - 15 mV). The intensity of FTIR peaks of silica NPs has been significantly decreased upon gold coating indicating a successful attachment of the gold thin layer. The drug release was slightly faster from coated compared to uncoated NPs but both slower than free QC. The percentages of their cell toxicity were almost the same but lower than free QC and generally were higher against HeLa cells compared to fibroblast cells. Both nanosystems could be considered as promising nanocarriers with reasonable EE, slower release rate and lower toxicity compared to the free drug

Chitosan and Starch-Based Hydrogels Via Graft Copolymerization

Graft copolymerization is an attractive method for surface functionalization of natural polymers and can be initiated by chemical methods, radiation technique, and other systems. Polymer grafting onto polysaccharides is an effective method for the synthesis of superabsorbents. Depending upon the type of monomers and the conditions employed the properties of graft copolymers vary to a large extent. Chitosan is a nontoxic, biocompatible polysaccharide, and starch is a natural hydrophilic biopolymer. Both these are most abundant natural organic materials which are extensively investigated in the development of biodegradable and environment-friendly materials. Their hydrogels are of utmost importance for wide use in many fields including structural transplants, target drug delivery, tissue engineering, biosensors, adsorbents, etc. In this chapter, the various techniques used for the synthesis of chitosan/starch graft copolymers, their properties and possible applications are discussed in detail

STANDARIZED ECONOMICAL STUDY OF EGYPTIAN OILS AND FATS DEMAND FACTORS

Recent data shows a declining performance in Egyptian foreign trade of agricultural sector especially by increased the Egyptian agricultural import, which leads to increase the deficit of agricultural trade balance. Agricultural processed proucts is consider one of main source of agricultural trade balance deficit. As a result, it is necessary to study the main factors affecting the Egyptian demand of foreign agricultura processed products to determine the most important factors and determine the possible solution in future. It is clear through the research resealt of the factors affecting the quantity of the Egyptian fats and oil imports, that their main factors are determined by the total production of the Egyptian fats and oil, the imports price and the consumption of oils and fats and in the total exports of USA from oil and fats. Moreover, it is obvious from the factors affecting the demand in Egyptian market that the price of the USA fats and oil is considered the main factor in most of these markets. Moreover the effect of free trade agreement and European Egyptian agreement were significant positive for increase the Egyptian demand of foreign oils and fats. So it is important to use some possible solution for decreasing these positive impact of these fators on Egyptian demand for foreign oils and fats for developing countries without being against the activation of these agreements. It is important to control the national consumption and increase the total production of oil and fats

Insinuation Salmonella Typhi for 23Na and 60Co Radioactive Sources

The study investigates the effect of radioactive sources on Salmonella typhi, a gram-negative, rod-shaped bacterium that infects various cell types. Salmonella primarily spreads through contaminated food, commonly tainted by human or animal waste. In this descriptive research, 50 S. typhi samples from diverse patients at Baghdad Hospital were utilized. The method involved identifying the bacteria using Viteck2, culturing them on Nutrient broth and Nutrient agar, and exposing them to various radioactive sources: Na23 and CO60 (1 µCi and CO60 10 µCi). Petri dishes containing Nutrient agar were used for sample plating, followed by incubation for 24 hours at 37°C. The results showed that exposure to beta and gamma rays emitted by Na23 at an activity level of 10 µCi resulted in increased radiation levels with time. Without Almmonium, the radiation doses for 1, 2, and 3 hours were 9.64 mSv, 19.29 mSv, and 28.93 mSv, respectively, leading to varying viable cell counts. With Almmonium, the doses for the same periods were significantly lower, with fewer viable cells. Similarly, exposure to CO60 (1 µCi) resulted in varied radiation levels and viable cell counts, indicating a similar trend with and without Almmonium. CO60 (10 µCi) also demonstrated an increase in radiation doses over time, displaying an impact on viable cell counts. The study concludes that radiation emitted from Sodium and Cobalt radioactive sources exhibited effectiveness in eliminating S. typhi in direct proportion to increased exposure time and dose. This research highlights the potential for radioactive sources to impact the viability of Salmonella typhi cells

Recurrence and progression in nonmuscle invasive transitional cell carcinoma of urinary bladder treated with intravesical Bacillus Calmette–Guerin: A single center experience and analysis of prognostic factors

Background: Intravesical Bacillus Calmette–Guerin (BCG) has been the standard of care for the prevention of nonmuscle invasive bladder cancer (NMIBC) recurrence following resection. Attempts to improve on the result by combining it with other agents have largely failed. This study addresses the result of BCG therapy in our patient population and compares the result with our combination BCG and interferon therapy published earlier. Materials and Methods: The medical records of patients diagnosed with NMIBC and treated with transurethral resection and intravesical BCG were reviewed. Univariate analysis was performed on most known prognostic factors. Results were compared to published data on the use of BCG and interferon from the same institution. Results: Thirty-one patients were identified. Median age was 66 (range 33–109), 80.6% were males. Fourteen patients (45%) had ≤ 3 tumors and 18 (58.1%) had T1 lesions. Four patients (12.9%) had Grade 3 tumors and 25 (80.6%) had Grade 2 tumors. One patient (3.2) had concurrent carcinoma in situ and 11 (35.5%) were treated upon initial diagnosis. At 5 years, the relapse-free survival was 61.3% (95% confidence interval (CI) 44.2–78.4%), progression-free survival was 85.6% (95% CI 73.3–97.9%), and overall survival was 93% (95% CI 84.1–100%). Comparison with the BCG and interferon data showed no significant difference. Conclusion: The result of BCG therapy in our patient population is similar to western reported data. Efficacy of BCG alone is equal to BCG and interferon within our institution