Antibacterial effect of orange Monascus pigment against Staphylococcus aureus

The objective of this work was to research the antibacterial effects of orange pigment, which was separated from Monascus pigments, against Staphylococcus aureus. The increase of the diameter of inhibition zone treated with orange pigment indicated that orange pigment had remarkable antibacterial activities against S. aureus. Orange pigment (10 mg ml−1) had a strong destructive effect on the membrane and structure of S. aureus by the analysis of scanning electron microscopy as well as transmission electron microscopy. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) further demonstrated that the cell membrane was seriously damaged by orange pigment, which resulted in the leakage of protein from S. aureus cells. A significant decrease in the synthesis of DNA was also seen in S. aureus cells exposed to 10 mg ml−1 orange pigment. All in all, orange pigment showed excellent antibacterial effects against S. aureus

Optimization of protein extraction from tree peony (Paeonia suffruticosa Andr.) seed using response surface methodology

Optimization of extraction ratio (ER) of tree peony seed protein (TPSP) was investigated using response surface methodology (RSM). The second-degree equation for ER of TPSP had high coeffi cient (0.9625) of determination. The probability (P) value of regression model signifi cance was less than 0.001 by analysis of central composite rotatable design. Relationships of ER to pH, liquid/solid ratio, squares of all factors, and cross-product factors (x2x3, x2x4, x3x4) were signifi cant (P1x2, x1x3, x1x4) were not signifi cant factors (P>0.05). Optimum extraction conditions were 3.42 h, pH 9.50, 50.80 ºC, and 9.54 ml g–1 of liquid/solid ratio with the maximum ER (43.60%) . SDS-PAGE indicated TPSP had mainly four proteins (180, 100, 60, and 35 kDa) with four subunits of 60, 48, 38, and 23 kDa. TPSP had a good amino acid composition with abundant essential amino acids (39.76%) determined by amino acid analysis

Mixed symmetry superconductivity in two-dimensional Fermi liquids

We consider a 2D isotropic Fermi liquid with attraction in both $s$ and $d$ channels and examine the possibility of a superconducting state with mixed $s$ and $d$ symmetry of the gap function. We show that both in the weak coupling limit and at strong coupling, a mixed $s+id$ symmetry state is realized in a certain range of interaction. Phase transitions between the mixed and the pure symmetry states are second order. We also show that there is no stable mixed $s+d$ symmetry state at any coupling.Comment: 3 figures attached in uuencoded gzipped file

Low field vortex matter in YBCO: an atomic beam magnetic resonance study

We report measurements of the low field structure of the magnetic vortex lattice in an untwinned YBCO single-crystal platelet. Measurements were carried out using a novel atomic beam magnetic resonance (ABMR) technique. For a 10.7 G field applied parallel to the c-axis of the sample, we find a triangular lattice with orientational order extending across the entire sample. We find the triangular lattice to be weakly distorted by the a-b anisotropy of the material and measure a distortion factor, f = 1.16. Model-experiment comparisons determine a penetration depth, lambda_ab = 140 (+-20) nm. The paper includes the first detailed description of the ABMR technique. We discuss both technical details of the experiment and the modeling used to interpret the measurements.Comment: 44 pages, 13 figures, submitted to Phys. Rev. B Revision includes Postscript wrapped figures + minor typo

Insights from a new method providing single-shot, planar measurement of gas-phase temperature in particle-laden flows under high-flux radiation

Published online: 31 March 2021Two-colour laser-induced fluorescence (LIF) of toluene has been demonstrated to provide in situ, spatially resolved, planar measurements of the gas-phase temperature in a particle-laden flow with strong radiative heating at fluxes up to 42.8 MW/m². Toluene was seeded in trace quantities into the gas flow laden with particles of mean diameter 173 μm at a volumetric loading sufficiently high for particle–fluid and particle–particle interactions to be significant. The particle number density was also measured simultaneously using Mie scattering. The two-colour LIF method was found to resolve temperature with a pixelto- pixel standard deviation of 17.8 °C for unheated measurements in this system despite significant attenuation of the probe laser and signal trapping of the fluorescence emissions from the densely loaded particles. Following heating of the particles using high flux radiation, the increase in the gas-phase temperature from convection was found to be spatially non-uniform with highly localised regions of temperature spanning from ambient to 150 °C. This gas-phase heating continued well downstream from the limits of the region with radiative heating, with the time-averaged gas temperature increasing with distance at up to 2,200 °C/m on the jet centreline. The temperature of the flow was non-symmetrical in the direction of the heating beam, because the particles attenuate the radiation through absorption and scattering. The addition of radiation at fluxes up to 42.8 MW/m² did not significantly change the particle number density distribution within the region investigated here.Elliott W. Lewis, Timothy C. W. Lau, Zhiwei Sun, Zeyad T. Alwahabi, Graham J. Natha

A computational study of positive streamers interacting with dielectrics

We use numerical simulations to study the dynamics of surface discharges, which are common in high-voltage engineering. We simulate positive streamer discharges that propagate towards a dielectric surface, attach to it, and then propagate over the surface. The simulations are performed in air with a two-dimensional plasma fluid model, in which a flat dielectric is placed between two plate electrodes. Electrostatic attraction is the main mechanism that causes streamers to grow towards the dielectric. Due to the net charge in the streamer head, the dielectric gets polarized, and the electric field between the streamer and the dielectric is increased. Compared to streamers in bulk gas, surface streamers have a smaller radius, a higher electric field, a higher electron density, and higher propagation velocity. A higher applied voltage leads to faster inception and faster propagation of the surface discharge. A higher dielectric permittivity leads to more rapid attachment of the streamer to the surface and a thinner surface streamer. Secondary emission coefficients are shown to play a modest role, which is due to relatively strong photoionization in air. In the simulations, a high electric field is present between the positive streamers and the dielectric surface. We show that the magnitude and decay of this field are affected by the positive ion mobility

Soot structure and flow characteristics in turbulent non-premixed methane flames stabilised on a bluff-body

The soot properties of methane in turbulent regimes are not well characterised but are highly desirable. Methane is the main constituent of natural gas that is broadly used in many industrial combustors. Investigation of turbulent methane flames under well-defined boundary conditions is therefore useful for interpreting soot formation in practical burners and can be used for further model development. This study presents a joint experimental and numerical study of a series of turbulent non-premixed bluff-body flames fuelled with pure methane for three values of the momentum flux ratio of fuel jet to co-flowing air. Soot volume fraction (SVF) and flowfield are measured simultaneously using planar laser-induced incandescence (P-LII) and 2D-polarised particle image velocimetry (P-PIV). Additionally, time-averaged temperature, mixture fraction, OH and C2H2 concentrations are estimated numerically using RANS models. The global flame structure for all three flames features a recirculation zone with a double-vortex structure, a jet-propagating zone, and a neck zone connecting the two regions. The soot distribution within the recirculation zone shows clear distinct features, which is attributed to the mean mixture fraction distribution in this zone. Increasing the momentum flux ratio shifts the location of the mean stoichiometric mixture fraction to the rich inner vortex core, leading to a distinct peak of the total integrated soot in the inner vortex of the recirculation zone that is not observed in other cases. Also, it is deduced that the soot inception starts earlier in the recirculation zone for the flame with the highest momentum flux ratio and in the jet zone for the other two flames. Much higher soot concentration and lower intermittency are found with ethylene-based flames stabilised on the same burner and with the same operating conditions. In addition, the study has generated a database of soot and flowfield results, which can be helpful for future model validations.Amir Rowhani, Zhiwei Sun, Alfonso Chinnici, Paul R. Medwell, Graham J. Nathan, Bassam B. Dall

Multiplicity Studies and Effective Energy in ALICE at the LHC

In this work we explore the possibility to perform ``effective energy'' studies in very high energy collisions at the CERN Large Hadron Collider (LHC). In particular, we focus on the possibility to measure in $pp$ collisions the average charged multiplicity as a function of the effective energy with the ALICE experiment, using its capability to measure the energy of the leading baryons with the Zero Degree Calorimeters. Analyses of this kind have been done at lower centre--of--mass energies and have shown that, once the appropriate kinematic variables are chosen, particle production is characterized by universal properties: no matter the nature of the interacting particles, the final states have identical features. Assuming that this universality picture can be extended to {\it ion--ion} collisions, as suggested by recent results from RHIC experiments, a novel approach based on the scaling hypothesis for limiting fragmentation has been used to derive the expected charged event multiplicity in $AA$ interactions at LHC. This leads to scenarios where the multiplicity is significantly lower compared to most of the predictions from the models currently used to describe high energy $AA$ collisions. A mean charged multiplicity of about 1000-2000 per rapidity unit (at $\eta \sim 0$) is expected for the most central $Pb-Pb$ collisions at $\sqrt{s_{NN}} = 5.5 TeV$.Comment: 12 pages, 19 figures. In memory of A. Smirnitski

The newly observed open-charm states in quark model

Comparing the measured properties of the newly observed open-charm states D(2550), D(2600), D(2750), D(2760), D_{s1}(2710), D_{sJ}(2860), and D_{sJ}(3040) with our predicted spectroscopy and strong decays in a constituent quark model, we find that: (1) the D(2\,^1S_0) assignment to D(2550) remains open for its too broad width determined by experiment; (2) the D(2600) and $D_{s1}(2710)$ can be identified as the 2\,^3S_1-1\,^3D_1 mixtures; (3) if the D(2760) and D(2750) are indeed the same resonance, they would be the D(1\,^3D_3); otherwise, they could be assigned as the D(1\,^3D_3) and $D^\prime_2(1D)$, respectively; (4) the $D_{sJ}(2860)$ could be either the $D_{s1}(2710)$'s partner or the D_s(1\,^3D_3); and (5) both the $D_{s1}(2P)$ and $D^\prime_{s1}(2P)$ interpretations for the $D_{sJ}(3040)$ seem likely. The $E1$ and $M1$ radiative decays of these sates are also studied. Further experimental efforts are needed to test the present quarkonium assignments for these new open-charm states.Comment: 26 pages,7 figures, journal versio

Coherent Phonon Dynamics in Short-Period InAs/GaSb Superlattices

We have performed ultrafast pump-probe spectroscopy studies on a series of InAs/GaSb-based short-period superlattice (SL) samples with periods ranging from 46 \AA to 71 \AA. We observe two types of oscillations in the differential reflectivity with fast ($\sim$ 1- 2 ps) and slow ($\sim$ 24 ps) periods. The period of the fast oscillations changes with the SL period and can be explained as coherent acoustic phonons generated from carriers photoexcited within the SL. This mode provides an accurate method for determining the SL period and assessing interface quality. The period of the slow mode depends on the wavelength of the probe pulse and can be understood as a propagating coherent phonon wavepacket modulating the reflectivity of the probe pulse as it travels from the surface into the sample.Comment: 6 pages, 4 figure