Long-term cultures of murine fetal liver retain very early B lymphoid phenotype.

Long-term cultures of murine fetal liver have been successfully established using a modification of our in vitro bone marrow culture system (14, 15). Fetal liver cells from midgestation BALB/c embryos were plated onto BAB-14 bone marrow stromal cell-adherent layers. After a 3-5 wk period, cell growth began to increase and these cells were expanded in number on fresh feeder layers. The cultured fetal liver cells were lymphoid in morphology, 5-20% cytoplasmic Ig-positive, but less than 1% surface Ig-positive. Southern blot analysis of the cultured fetal liver cells, as well as cultured bone marrow-derived B cells, demonstrated a population with germline Ig heavy chain loci, possibly representing very early B cell precursors. Abelson murine leukemia virus (A-MuLV) clonal transformants of such cultured fetal liver cells had a phenotypic distribution similar to that seen with fresh fetal liver transformants but distinct from those obtained with the transformation of either cultured or fresh bone marrow. All A-MuLV transformants isolated had rearrangements at the mu heavy chain locus of both chromosomes, irrespective of Ig production. In addition, most mu heavy chain producers had at least one rearranged kappa gene locus. These long-term fetal liver cultures provide large numbers of cells for studying events early in the B lymphocyte lineage. The cultured fetal liver cells retained phenotypic traits similar to fresh fetal liver B cells and distinctive from bone marrow cells cultured under similar conditions

Grain refinement in a AlZnMgCuTi alloy by intensive melt shearing: A multi-step nucleation mechanism

This is a post-print version of the article. Copyright @ 2010 Elsevier B.V.Direct chill (DC) cast ingots of wrought Al alloys conventionally require the deliberate addition of a grain refiner to provide a uniform as-cast microstructure for the optimisation of both mechanical properties and processability. Grain refiner additions have been in widespread industrial use for more than half a century. Intensive melt shearing can provide grain refinement without the need for a specific grain refiner addition for both magnesium and aluminium based alloys. In this paper we present experimental evidence of the grain refinement in an experimental wrought aluminium alloy achieved by intensive melt shearing in the liquid state prior to solidification. The mechanisms for high shear induced grain refinement are correlated with the evolution of oxides in alloys. The oxides present in liquid aluminium alloys, normally as oxide films and clusters, can be effectively dispersed by intensive shearing and then provide effective sites for the heterogeneous nucleation of Al3Ti phase. As a result, Al3Ti particles with a narrow size distribution and hence improved efficiency as active nucleation sites of alpha-aluminium grains are responsible for the achieved significant grain refinement. This is termed a multi-step nucleation mechanism.Funding was obtained from the EPRSC

Nonperturbative renormalization in a scalar model within Light-Front Dynamics

Within the covariant formulation of Light-Front Dynamics, in a scalar model with the interaction Hamiltonian $H=-g\psi^{2}(x)\phi(x)$, we calculate nonperturbatively the renormalized state vector of a scalar "nucleon" in a truncated Fock space containing the $N$, $N\pi$ and $N\pi\pi$ sectors. The model gives a simple example of non-perturbative renormalization which is carried out numerically. Though the mass renormalization $\delta m^2$ diverges logarithmically with the cutoff $L$, the Fock components of the "physical" nucleon are stable when $L\to\infty$.Comment: 22 pages, 5 figure

Polymeric blends for sensor and actuation dual functionality

The invention described herein supplies a new class of electroactive polymeric blend materials which offer both sensing and actuation dual functionality. The blend comprises two components, one component having a sensing capability and the other component having an actuating capability. These components should be co-processable and coexisting in a phase separated blend system. Specifically, the materials are blends of a sensing component selected from the group consisting of ferroelectric, piezoelectric, pyroelectric and photoelectric polymers and an actuating component that responds to an electric field in terms of dimensional change. Said actuating component includes, but is not limited to, electrostrictive graft elastomers, dielectric electroactive elastomers, liquid crystal electroactive elastomers and field responsive polymeric gels. The sensor functionality and actuation functionality are designed by tailoring the relative fraction of the two components. The temperature dependence of the piezoelectric response and the mechanical toughness of the dual functional blends are also tailored by the composition adjustment

How content contributors assess and establish credibility on the web

The proliferation of user‐generated content (UGC) is one of the distinguishing characteristics of Web 2.0. Internet users contribute content online through platforms such as blogs, wikis, video sharing sites, and sites that allow user feedback. Yet little is known of the credibility practices of these content contributors. Through phone interviews conducted with 29 online content contributors, this study investigates how content contributors assess credibility when gathering information for their online content creation and mediation activities, as well as the strategies they use to establish the credibility of the content they create. These contributors reported that they engaged in content creation activities such as posting or commenting on blogs or online forums, rating or voting on online content, and uploading photos, music, or video. We found that credibility judgments made when gathering information for online content creation and mediation activities could be grouped into three levels: intuitive, heuristic, and strategy‐based. We identified three distinctive ways of establishing credibility that are applied during different phases of content contribution: ensuring credibility during the content creation phase; signaling credibility during the content presentation phase; and reinforcing credibility during the post‐production phase. We also discovered that content contributors tend to carry over the strategies they used for assessing credibility during information gathering to their strategies for establishing the credibility of their own content. Theoretical implications for credibility research and practical implications for developing information literacy programs are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90253/1/14504801163_ftp.pd

A diary study of credibility assessment in everyday life information activities on the web: Preliminary findings

This study investigates how people's credibility assessment processes have evolved as they engage in increasingly diverse types of online activities beyond seeking for information or reading online news. Using an online activity diary method, information on people's online activities and their associated credibility assessment processes were collected at multiple points throughout the day for three days. This paper reports on a preliminary analysis of 2,471 diary entries received from 333 respondents. Content analysis was applied to people's descriptions of their online activities, yielding 17 different types of information objects and 26 categories of online content. People's credibility judgments were examined on three levels: construct, heuristics, and interaction. The results, although preliminary, indicate that distinct credibility assessment heuristics are in fact emerging as people engage in online activities involving more user-generated and multimedia content. The unique contribution of this paper is its identification of the importance of taking a heuristic approach to credibility assessment by studying a large sample of heavy Internet users within the context of the everyday life information activities they conduct online.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/83172/1/14504701182_ftp.pd

An online activity diary method for studying credibility assessment on the Web

No Abstract.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78324/1/1450460388_ftp.pd

Renormalized Effective QCD Hamiltonian: Gluonic Sector

Extending previous QCD Hamiltonian studies, we present a new renormalization procedure which generates an effective Hamiltonian for the gluon sector. The formulation is in the Coulomb gauge where the QCD Hamiltonian is renormalizable and the Gribov problem can be resolved. We utilize elements of the Glazek and Wilson regularization method but now introduce a continuous cut-off procedure which eliminates non-local counterterms. The effective Hamiltonian is then derived to second order in the strong coupling constant. The resulting renormalized Hamiltonian provides a realistic starting point for approximate many-body calculations of hadronic properties for systems with explicit gluon degrees of freedom.Comment: 25 pages, no figures, revte

Lignin biomarkers as tracers of mercury sources in lakes water column

This study presents the role of specific terrigenous organic compounds as important vectors of mercury (Hg) transported from watersheds to lakes of the Canadian boreal forest. In order to differentiate the autochthonous from the allochthonous organic matter (OM), lignin derived biomarker signatures [Lambda, S/V, C/V, P/(V ? S), 3,5-Bd/V and (Ad/Al)v] were used. Since lignin is exclusively produced by terrigenous plants, this approach can give a non equivocal picture of the watershed inputs to the lakes. Moreover, it allows a characterization of the source of OM and its state of degradation. The water column of six lakes from the Canadian Shield was sampled monthly between June and September 2005. Lake total dissolved Hg concentrations and Lambda were positively correlated, meaning that Hg and ligneous inputs are linked (dissolved OM r2 = 0.62, p\0.0001; particulate OM r2 = 0.76, p\0.0001). Ratios of P/(V ? S) and 3,5-Bd/V from both dissolved OM and particulate OM of the water column suggest an inverse relationship between the progressive state of pedogenesis and maturation of the OM in soil before entering the lake, and the Hg concentrations in the water column. No relation was found between Hg levels in the lakes and the watershed flora composition—angiosperm versus gymnosperm or woody versus non-woody compounds. This study has significant implications for watershed management of ecosystems since limiting fresh terrestrial OM inputs should reduce Hg inputs to the aquatic systems. This is particularly the case for largescale land-use impacts, such as deforestation, agriculture and urbanization, associated to large quantities of soil OM being transferred to aquatic systems

The potential of new tumor endothelium-specific markers for the development of antivascular therapy.

Angiogenesis is a hallmark of solid tumors, and disruption of tumor vasculature is an active anticancer therapy in some cases. Several proteins expressed on the surface of tumor endothelium have been identified during the last decade. However, due to the expression in both physiological and tumor angiogenesis, only a few targets have been developed for clinical therapeutics. By thorough SAGE analysis of mouse endothelial cells isolated from various normal resting tissues, regenerating liver, and liver-metastasized tumor, Seaman and colleagues in this issue of Cancer Cell have demonstrated organ-specific endothelial markers, physiological angiogenesis endothelial markers, and tumor endothelial markers and revealed striking differences between physiological and pathological angiogenesis