Arbuscular Mycorrhizal Fungi and Plant Chemical Defence : Effects of Colonisation on Aboveground and Belowground Metabolomes

Arbuscular mycorrhizal fungal (AMF) colonisation of plant roots is one of the most ancient and widespread interactions in ecology, yet the systemic consequences for plant secondary chemistry remain unclear. We performed the first metabolomic investigation into the impact of AMF colonisation by Rhizophagus irregularis on the chemical defences, spanning above- and below-ground tissues, in its host-plant ragwort (Senecio jacobaea). We used a non-targeted metabolomics approach to profile, and where possible identify, compounds induced by AMF colonisation in both roots and shoots. Metabolomics analyses revealed that 33 compounds were significantly increased in the root tissue of AMF colonised plants, including seven blumenols, plant-derived compounds known to be associated with AMF colonisation. One of these was a novel structure conjugated with a malonyl-sugar and uronic acid moiety, hitherto an unreported combination. Such structural modifications of blumenols could be significant for their previously reported functional roles associated with the establishment and maintenance of AM colonisation. Pyrrolizidine alkaloids (PAs), key anti-herbivore defence compounds in ragwort, dominated the metabolomic profiles of root and shoot extracts. Analyses of the metabolomic profiles revealed an increase in four PAs in roots (but not shoots) of AMF colonised plants, with the potential to protect colonised plants from below-ground organisms

Interaction of 8-Hydroxyquinoline with Soil Environment Mediates Its Ecological Function

Background: Allelopathic functions of plant-released chemicals are often studied through growth bioassays assuming that these chemicals will directly impact plant growth. This overlooks the role of soil factors in mediating allelopathic activities of chemicals, particularly non-volatiles. Here we examined the allelopathic potential of 8-hydroxyquinoline (HQ), a chemical reported to be exuded from the roots of Centaurea diffusa. Methodology/Principal Findings: Growth bioassays and HQ recovery experiments were performed in HQ-treated soils (non-sterile, sterile, organic matter-enriched and glucose-amended) and untreated control soil. Root growth of either Brassica campestris or Phalaris minor was not affected in HQ-treated non-sterile soil. Soil modifications (organic matter and glucose amendments) could not enhance the recovery of HQ in soil, which further supports the observation that HQ is not likely to be an allelopathic compound. Hydroxyquinoline-treated soil had lower values for the CO2 release compared to untreated non-sterile soil. Soil sterilization significantly influenced the organic matter content, PO 4-P and total organic nitrogen levels. Conclusion/Significance: Here, we concluded that evaluation of the effect of a chemical on plant growth is not enough in evaluating the ecological role of a chemical in plant-plant interactions. Interaction of the chemical with soil factors largel

Plant–soil feedback of native and range-expanding plant species is insensitive to temperature

Temperature change affects many aboveground and belowground ecosystem processes. Here we investigate the effect of a 5°C temperature increase on plant–soil feedback. We compare plant species from a temperate climate region with immigrant plants that originate from warmer regions and have recently shifted their range polewards. We tested whether the magnitude of plant–soil feedback is affected by ambient temperature and whether the effect of temperature differs between these groups of plant species. Six European/Eurasian plant species that recently colonized the Netherlands (non-natives), and six related species (natives) from the Netherlands were selected. Plant–soil feedback of these species was determined by comparing performance in conspecific and heterospecific soils. In order to test the effect of temperature on these plant–soil feedback interactions, the experiments were performed at two greenhouse temperatures of 20/15°C and 25/20°C, respectively. Inoculation with unconditioned soil had the same effect on natives and non-natives. However, the effect of conspecific conditioned soil was negative compared to heterospecific soil for natives, but was positive for non-natives. In both cases, plant–soil interactions were not affected by temperature. Therefore, we conclude that the temperature component of climate change does not affect the direction, or strength of plant–soil feedback, neither for native nor for non-native plant species. However, as the non-natives have a more positive soil feedback than natives, climate warming may introduce new plant species in temperate regions that have less soil-borne control of abundance

Millennial-scale glacial variability versus Holocene stability: changes in planktic and benthic foraminifera faunas and ocean circulation in the North Atlantic during the last 60000 years.

Two piston cores, DS97-2P from the Reykjanes Ridge in the central North Atlantic Ocean (1685 m water depth) and ENAM33 from southwest of the Faeroe Islands in the NE Atlantic (1217 m water depth), have been investigated for their planktic and benthic foraminiferal content. DS97-2P is situated near the Subarctic Front and productivity measured by accumulation rates of benthic and planktic foraminifera has been generally high during the Holocene. The productivity shows a clear decrease from an early Holocene maximum to a late Holocene minimum. Coeval changes in the benthic faunas indicate that the food supply changed from large, irregular pulses during the early Holocene to a more sustained flux during the late Holocene. Presumably in concert with decreasing bottom current activity oxygen conditions in the bottom water became poorer. Another feature of the late Holocene is an increasing instability of the North Atlantic thermohaline circulation regime. Nevertheless, the changes in faunal composition and productivity during the Holocene were gradual as compared to the discontinuous distribution patterns and abrupt productivity shifts during the glacial. The glacial shifts were on a millennial time scale and correlate with the interstadial-stadial phases of the Dansgaard-Oeschger cycles in the Greenland ice cores. The faunas of the warm interstadial phases resembled the Holocene faunas, and both surface and bottom productivity was high. The faunas suggest that the interstadial circulation pattern was very similar to the modern system with convection in the Nordic seas and generation of North Atlantic Deep Water. The planktic faunas during the cold stadials and Heinrich events were completely dominated by the polar species Neogloboquadrina pachyderma s, and surface conditions were cold and the productivity low. The benthic faunas were dominated by species that presently thrive in areas with a low amount of food and reduced oxygen content. The water column was probably stratified with low saline, cold surface water overlying poorly aerated, intermediate water masses

Reconstruction of the late-Holocene changes in the Sub-Arctic Front position at the Reykjanes Ridge, north Atlantic

International audienceChanges in the dynamics of the North Atlantic subpolar gyre are involved in the modulation of the northward salinity and heat transport in the northern North Atlantic via the North Atlantic Current (NAC). Variations in the strength of the East Greenland Current (EGC) can influence the gyre dynamics by impacting deep convection in the Labrador Sea. Oxygen isotope data of three planktonic foraminiferal species (surface water Neogloboquadrina pachyderma dextral coiling and Globigerina bulloides; thermocline recorder Globorotalia inflata) from a site located close to the present Sub-Arctic Front at the Reykjanes Ridge suggest significant strengthening or shifting of the Sub-Arctic Front throughout the late Holocene. The oxygen isotope based inferences are supported by Mg/Ca-derived temperature reconstructions from Neogloboquadrina pachyderma dextral coiling, alkenone-derived sea surface temperature and other paleoclimatic proxy data. The late-Holocene strengthening/shift of the Sub-Arctic Front appears caused by an increasingly more defined and fresher EGC. The proposed subpolar gyre changes may modulate the northward heat transport, and explain the geographically different long-term climatic trends in the North Atlantic during the late Holocene, i.e. a cooling of the EGC-influenced regions and a warming of the NAC-influenced areas from c. 4 to 5 ka. This mechanism cannot, however, explain the simultaneous occurrence of millennial-scale events at c. 5.6, 3.9, 2.7, 1.3 ka and the ‘Little Ice Age’ in both areas. Noteworthy is the steadily increasing amplitude of these cold events at the Reykjanes Ridge, likely induced by drift ice and/or EGC-influence culminating in the ‘Little Ice Age’. A widespread pronounced warming at 2.0 ka seems to represent the ‘Roman Warm Period’ and reflects the warmest period of the late Holocene

The Faroe-Shetland Gateway: Late Quaternary water mass exchange between the Nordic seas and the northeastern Atlantic.

Thirteen piston and gravity cores from the Faroe-Shetland area were investigated for their planktic and benthic foraminiferal and oxygen isotopic distributions. Eight time-slices between 18 ka BP and the present were reconstructed to study variations in surface and deep water exchange between the SE Norwegian Sea and the northeast Atlantic Ocean. Today, a relatively strong northward flow of warm North Atlantic surface water is counterbalanced by a southward outflow of newly convected cold bottom water, the Norwegian Sea Overflow Water. During the last glacial maximum at 18 ka BP both the surface and bottom flows were slow and the climate conditions were Arctic. The convection north of the Faroe area was weak and unstable. The first indication of the deglaciation is a decrease in the planktic oxygen isotope values discernible southwest of the Faroe Islands at 15.5 ka BP. The deglaciation proceeded northeast and eastward synchronous with a gradual intensification of northward flowing warmer Atlantic Intermediate Water along the sea bottom. Meltwater fluxes increased between 14 and 13 ka BP producing cold surface waters, and the climatic cooling was extreme. There was no southward overflow of cold bottom water during this time period and the exchange of water masses between the Nordic seas and the North Atlantic Ocean was essentially reversed, i.e. estuarine. During the Bolling Interstadial at 12.5 ka BP northward flowing warm surface water was present to the east of the Faroe-Shetland Channel, wedged below a tongue of polar water spreading from the northwest and reaching into the Faroe-Shetland Channel. Convection in the Nordic seas and overflow of cold deep water started during the Bolling Interstadial. The polar water spread more eastward and southward during the following cold spell, the Younger Dryas, around 10.3 ka BP. The polar water was overlying the warmer, but more saline Atlantic water, which flowed northward below the cold surface water. The overflow of cold bottom water was supposedly only slightly weaker than during the Bolling Interstadial. Strong inflow of warm surface water took place during the Early Holocene at 9.5 ka BP and relatively dense cold water flowed southward along the bottom. The rate of water mass exchange reached a maximum at 6.5 ka BP, when both the inflow of warm Atlantic surface water and the outflow of cold dense bottom water appear to have been stronger than today

Proof Theoretic Complexity

A weak formal theory of arithmetic is developed, entirely analogous to classical arithmetic but with two separate kinds of variables: induction variables and quantifier variables. The point is that the provably recursive functions are now more feasibly computable than in the classical case, lying between Grzegorczyk's E² and E³, and their computational complexity can be characterized in terms of the logical complexity of their termination proofs. Previous results of Leivant are reworked and extended in this new setting, with quite di#erent proof theoretic methods