Taxonomy and evolutionary relationships of Passiflora subg. Decaloba supersect. Decaloba sect. Xerogona (Passifloraceae): contributions of palynological, morphological and molecular studies

Passiflora subg. Decaloba supersect. Decaloba sect. Xerogona (Passifloraceae) is a tropical and subtropical group comprising 14 species that occur in tropical biomes throughout Latin America, including the Atlantic Forest. The section Xerogona comprises herbaceous vines characterized by a lack of petiole glands on their leaves, of bracts and of ocelli on their leaf blades, as well as by their capsular fruits. We analyzed the phylogeny on the basis of morphological characters (including pollen characters) and molecular data. The inferred phylogeny was used in order to characterize, circumscribe and delimit the section and the species. We examined the phylogenetic relationships among the species, evaluating the circumscription of the section on the basis of the trnL-trnF intergenic spacer region of chloroplast DNA and the internal transcribed spacer region of nuclear ribosomal DNA. We constructed phylogenetic trees on the basis of the morphological and molecular data. We found that P. subg. Decaloba supersect. Decaloba sect. Xerogona appears to be monophyletic only in the molecular analyses. The phylogenetic analyses performed here also indicated that P. subg. Decaloba is monophyleti

Isolation And Characterization Of Microsatellite Loci In Paspalum Notatum Flüggé (poaceae)

Paspalum notatum is a forage grass recognized as one of the major constituents of the native grasslands in the New World. The knowledge of the genetic diversity and structure of P. notatum populations is fundamental for the conservation and germplasm management of this species. About 11 microsatellite markers were isolated from P. notatum and characterized in 25 accessions. The average number of alleles per locus was 7.9 and the PIC ranged from 0.36 to 0.89. The data demonstrated that the most of markers are suitable to detect polymorphism and to study the genetic diversity in the P. notatum species. Moreover, the transferability of these microsatellite were tested on other three congeneric species. © 2009 Springer Science+Business Media B.V.10619771980Billotte, N., Lagoda, P.J.R., Risterucci, A.M., Baurens, F.C., Microsatellite-enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Burton, G.W., Bahia grass types (1946) Agron J, 38, pp. 273-281Burton, G.W., The method of reproduction in common bahiagrass, Paspalum notatum (1948) Agron J, 40, pp. 443-452Chase, A., The North American species of Paspalum (1929) Contr. US Natl. Herb., 28, pp. 1-310Cidade, F.W., Dall'agnol, M., Bered, F., Souza-chies, T.T., Genetic diversity of the complex Paspalum notatum Flügge (Paniceae-Panicoideae) (2008) Genet Resour Crop Evol, 55, pp. 235-246Clayton, W.D., Renvoize, S., (1986) A. Genera Graminum: Grasse of the World, , London: Royal Botanic GardensCreste, S., Tulmann Neto, A., Figueira, A., Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Mol Biol Rep, 19, pp. 299-306Daurelio, L.D., Espinoza, F., Quarín, C.L., Pession, S.C., Genetic diversity in sexual diploid and apomictic tetraploid populations of Paspalum notatum situated in sympatry or allopatry (2004) Plant Syst Evol, 244, pp. 189-199Doyle, J.J., Doyle, J.L., A rapid DNA isolation procedure for small quantities of fresh leaf tissue (1987) Phytochemistry Bull, 19, pp. 11-15Quarín, C.L., Norrmann, G.A., Urbani, M.H., Polyploidization in aposporous Paspalum species (1989) Apomixis Newsl, 2, pp. 44-46Temnykh, S., DeClerck, G., Lukashova, A., Lipovich, L., Catinhour, S., McCouch, S., Computational and experimental analysis of microsatellites in rice (Oryza sativa 1.): Frequency, length variation, transposon associations, and genetic marker potential (2001) Genome, 11, pp. 1441-1452. , doi:10.1101/gr.184001Tessier, C., This, D.P., Boursiquot, J.M., Charrier, A., Optimization of the choice of molecular markers for varietal identification Vitis vinifera (1999) L. Theor Appl Genet, 98, pp. 171-177. , doi:10.1007/s00122005105