Knowledge and attitudes of physicians relating to reporting of adverse drug reactions in Sokoto, northwestern Nigeria

No Abstract

Activation of clay sample from Zaria L.G.A. of kaduna State and testing its bleaching performance on groundnut oil, palm oil and cottenseed oil

Clay samples from Zaria L.G.A. of Kaduna state was acid activated using hydrochloric acid in the concentration range of 5%, 10%, to 30% (v/v), at 90oC ± 2oC for 3 hours, and heat activated at 145oC for 30 minutes. The raw, acid and heat activated clay were tested on groundnut, palm and cottonseed oils. The per cent colour reduction was monitored spectrophotometrically. There was improvement in the decolourizing performance of the clay sample when acid or heat activated towards the three oil samples, but acid activation is more suitable for groundnut oil, while for palm and cottonseed oils the results obtained were similar for both methods. Keywords: Clay, Kaolinite, Bleaching, Zaria, Oi

Standalone General Purpose Data Logger Design and Implementation

This paper describes the design of a general purpose data logger that is compatible with a variety of transducers, potentially permitting the measurement and recording of a wide range of phenomena. The recorded data can be retrieved to a PC via an RS-232 serial port. The standalone general purpose data logger centered on a single microcontroller unit (MCU) the PIC18F4520. The circuit takes an input range of 15 – 30V DC in addition; an in-built 9V rechargeable battery provides backup power in the absence of an external source.  The microcontroller input form the Transduceroutput was conditioned within the allowable voltage range, the ADC calculates a binary value that is equivalent to its input (analog) voltage.  The in-built 10-bit ADC of the PIC18F4520 converts the analog input to a digital output by successive approximation, the 8 MSBs of the ADC are stored whereas the 2 LSBs are discarded which makes the output of the ADC varies from 0 to 255. Although the PIC18F4520 features 256 bytes of non-volatile EEPROM data memory, four AT24C256 2-wire serial EEPROM chips were used for data storage. The four chips were cascaded to form a memory bank that connects to the MCU via a common single 2-wire bus.The hardware employed to achieve the RS-232 serial interface was a 9-pin D-shell serial connector and a voltage level translator chip (Maxim’s MAX232). The connector simply allows the device to be coupled to a serial port of a PC using a standard serial cable. The TIMER1 module of the PIC18F4520 was configured such that its 16-bit register is incremented on the rising edge of the external clock signal applied to its input pin (pin 16). The input pins of the MCU that are dedicated to the switches are pulled up by resistors R11, R12, R13, R14, R15 and R16.  The push buttons temporarily pull down the input signal when depressed. The output devices consist of a “Liquid Crystal Display” (LCD) device, four general purpose LEDs, and a buzzer. The LCD displays the momentary status of the data-logger in terms of date, time, sampling rate and nature of logged data.  The LEDs are used to display the four MSBs of the measured data and the buzzer is used to raise an alarm if and when necessary but may be disabled in hardware by opening jumper J4. The design was implemented and simulated on Labcenterproteus and the components were mounted on a double layered PCB (Printed Circuit Board) that provides the tracks that make up the circuit.The prototype design realized was found to work satisfactorily.http://dx.doi.org/10.4314/njt.v34i2.18

The Influence of Metakaolin from Saaba (Burkina Faso) over Physico-Mechanical and Durability Properties of Mortars

This is the final version. Available on open access from Scientific Research Publishing via the DOI in this recordThe paper evaluates the feasibility of reducing clinker in the Portland Cement production using local metakaolin in Burkina Faso. Standardized testing methods have been used for this purpose, and experiments were performed on mortar prisms containing different amounts of metakaolin. Important results about the physical, mechanical and durability characterization of blended mortars were carried out in this study. The obtained results are discussed based on available literature data. These results have shown increased physical and durability properties for blended mortars. Although the mechanical strengths remained relatively low for higher MK incorporations, the latter grow to surpass these of PC mortars (the reference) at 28, 56 and 90 days of curing. The results in the paper, have confirmed the possibility of using metakaolin to partially substitute cement, a possibility to reduce the CO2 production by the cement industry in Burkina Faso.Deutscher Akademischer Austauschdiens

The pattern of infection and in vivo response to Chloroquine by uncomplicated Plasmodium falciparum malaria in northwestern Nigeria

The pattern of infection and in vivo response of uncomplicated Plasmodium falciparum malaria to Chloroquine as first line drug and Quinine, Halofantrine or Sulfadoxine-Pyrimethamine as second line medications was evaluated at nested sentinel points, including Government and Private Practices, for three consecutive months. 559 cases were evaluated of which 22.5% failed on Chloroquine therapy. The age range of P. falciparum malaria cases was 4 months to 48 years, with a mean and median age of 9.2 and 3 years, respectively. There were significantly more female patients than male. Also, ages 5 years and below accounted for 63.2% of cases and as a group had an increased risk of treatment failure with Chloroquine compared to older patients. In general, male patients also had a higher relative risk of treatment failure on Chloroquine. Patients treated in Government practices were more likely to fail than those treated in Private practices. All cases of failure to Chloroquine treatment responded to Quinine, Halofantrine or Sulfadoxine-Pyrimethamine. Key Words: Plasmodium falciparum malaria, Chloroquine, resistance. African Journal of Biotechnology Vol.4(1) 2005: 79-8

Hepatitis b, c and hiv serological markers in children with sickle cell anaemia in a tertiary hospital, Gusau, North-Western Nigeria

Sickle Cell Anaemia patients are considered to be among the high-risk groups for hepatitis B and C viral infections. These viruses and HIV share common routes of transmission and similar risk factors and their infections coexist. Objective: This study was aimed at determining the seroprevalence of hepatitis B, C and HIV viral markers of infections in children with SCA. Methodology: A cross sectional Hospital based study conducted on 89 confirmed SCA Children aged 6 months – 13 years in steady state attending Haematology Clinic in a Specialist Hospital Gusau from July 2017 to March 2018. Approval for the study was obtained from the Research and Ethics Committee of the Hospital. The age, gender, history of blood transfusion, traditional scarification, uvulectomy, circumcision and immunization of the subjects were recorded. Serological test was carried out to determine the prevalence of hepatitis B, C and HIV using the viral markers and HIV 1& 2 rapid test kits. Results: Eighty-nine subjects were recruited with 46(51.7%) males and 43(48.3%) females. The mean age was 5.06 ±3.4 years. The seroprevalence of HBsAg, HBsAb, HBcAb, HBeAg, HBeAb, AntiHCV and HIV 1& 2 were 3(3.4%), 3(3.4%), 5(5.7%), 1(1.1%), 6(6.9%) and 0% respectively. No co-infection among the studied subjects. There is no significant difference in the age or gender distribution and seroprevalence of the viruses among SCA children. p = > 0.05 Conclusion: Hepatitis C infection is found to be high as opposed to Hepatitis B which is lower among SCA Children in this community

Protocol For A Randomised, Multicentre, Double Blinded Phase III Study Of Perioperative Ketorolac In Women Of African Descent With Operable Breast Cancer

Cancer remains one of the leading causes of morbidity and mortality worldwide. Breast cancer is the most common form of malignancy occurring in women around the world. The aim of this present protocol is to outline the procedure for a randomised, multicentre, double blinded phase III study of perioperative Ketorolac in Women of African Descent with Operable Breast Cancer. The typical type of breast cancer in sub Saharan Africa is triple negative breast cancer (TNBC) and is usually considered the worst early breast cancer diagnosis since there are no known targeted therapies and patients often relapse and die early. sub-Saharan Africa seems the perfect place to conduct a randomized controlled double blinded clinical trial of perioperative NSAID Romano Demicheli 1, Erhabor Osaro 2, Michael Retsky 3 Forget Patrice 4.Vaidya Jayant S 5. Scientific Directorate Fondazione IRCCS Istituto Nazionale Tumori di Milano Italy 1, Usmanu Danfodiyo University, Sokoto, Nigeria 2, Harvard TH Chan School of Public Health Boston MA USA 3, Department of Anesthesiology, Universite catholique de Louvain, St-Luc Hospital, Av. Hippocrate 10-1821, 1200 Brussels, Belgium 4, University College Hospital, London, UK 5.etorolac to potentially reduce early relapses in breast cancer. The primary and secondary objectives of this trial are to evaluate and compare the Disease Free Survival (DFS) and Overall Survival (OS) of operable breast cancer patients randomised to standard treatment versus standard treatment plus perioperative Ketorolac and to compare the safety and tolerability of the treatment as well as to identify pre- and post-operative factors with prognostic relevance and establish correlations with clinical outcomes. It is anticipated that trial will be successful and would turn TNBC from the worst prognosis to the best and potentially help improve the quality of life of African women suffering from breast cancer

Sustainability Barriers in Nigeria Construction Practice

© 2022 IOP Publishing. Content from this work may be used under the terms of the Creative Commons Attribution 3.0. licence. https://creativecommons.org/licenses/by/3.0/The struggles to practise sustainable construction are not gaining the desired traction in Nigeria. This study established the likely barriers to successful application of sustainable construction in the Nigeria construction industry and factors to overcome the possible barriers. A quantitative approach was used for the study and a questionnaire survey was conducted among the professionals and other stakeholders. A descriptive method was used in analysing the collected data. Among the highly ranked sustainability barriers to construction practice are poor sustainability education in academic institutions, lack of incentives for designers to facilitate sustainable design, ignorance of lifecycle cost benefits, sustainable construction regarded as low priority and other issues take priority, and resistance to cultural change in the industry. The research recommends adequate sustainability education in academic institutions to positively impact the required cultural change in the industry. There is call for proper government policies that support implementation of sustainable construction practices. The study also advances the need for construction professionals and stakeholders to embrace the concept of sustainability education through continuing professional development and or postgraduate studies to improve the thinking and practicability of sustainable practice of construction in Nigeria.Peer reviewedFinal Published versio

Thermophilic PHP Protein Tyrosine Phosphatases (Cap8C and Wzb) from Mesophilic Bacteria

Protein tyrosine phosphatases (PTPs) of the polymerase and histidinol phosphatase (PHP) superfamily with characteristic phosphatase activity dependent on divalent metal ions are found in many Gram-positive bacteria. Although members of this family are co-purified with metal ions, they still require the exogenous supply of metal ions for full activation. However, the specific roles these metal ions play during catalysis are yet to be well understood. Here, we report the metal ion requirement for phosphatase activities of S. aureus Cap8C and L. rhamnosus Wzb. AlphaFold-predicted structures of the two PTPs suggest that they are members of the PHP family. Like other PHP phosphatases, the two enzymes have a catalytic preference for Mn2+, Co2+ and Ni2+ ions. Cap8C and Wzb show an unusual thermophilic property with optimum activities over 75 °C. Consistent with this model, the activity–temperature profiles of the two enzymes are dependent on the divalent metal ion activating the enzyme

Role of the C-Terminal β Sandwich of Thermoanaerobacter tengcongensis Thermophilic Esterase in Hydrolysis of Long-Chain Acyl Substrates

To search for a novel thermostable esterase for optimized industrial applications, esterase from a thermophilic eubacterium species, Thermoanaerobacter tengcongensis MB4, was purified and characterized in this work. Sequence analysis of T. tengcongensis esterase with other homologous esterases of the same family revealed an apparent tail at the C-terminal that is not conserved across the esterase family. Hence, it was hypothesized that the tail is unlikely to have an essential structural or catalytic role. However, there is no documented report of any role for this tail region. We probed the role of the C-terminal domain on the catalytic activity and substrate preference of T. tengcongensis esterase EstA3 with a view to see how it could be engineered for enhanced properties. To achieve this, we cloned, expressed, and purified the wild-type and the truncated versions of the enzyme. In addition, a naturally occurring member of the family (from Brevibacillus brevis) that lacks the C-terminal tail was also made. In vitro characterization of the purified enzymes showed that the C-terminal domain contributes significantly to the catalytic activity and distinct substrate preference of T. tengcongensis esterase EstA3. All three recombinant enzymes showed the highest preference for paranitrophenyl butyrate (pNPC4), which suggests they are true esterases, not lipases. Kinetic data revealed that truncation had a slight effect on the substrate-binding affinity. Thus, the drop in preference towards long-chain substrates might not be a result of substrate binding affinity alone. The findings from this work could form the basis for future protein engineering allowing the modification of esterase catalytic properties through domain swapping or by attaching a modular protein domain