Within-Home versus Between-Home Variability of House Dust Endotoxin in a Birth Cohort

Endotoxin exposure has been proposed as an environmental determinant of allergen responses in children. To better understand the implications of using a single measurement of house dust endotoxin to characterize exposure in the first year of life, we evaluated room-specific within-home and between-home variability in dust endotoxin obtained from 470 households in Boston, Massachusetts. Homes were sampled up to two times over 5–11 months. We analyzed 1,287 dust samples from the kitchen, family room, and baby’s bedroom for endotoxin. We fit a mixed-effects model to estimate mean levels and the variation of endotoxin between homes, between rooms, and between sampling times. Endotoxin ranged from 2 to 1,945 units per milligram of dust. Levels were highest during summer and lowest in the winter. Mean endotoxin levels varied significantly from room to room. Cross-sectionally, endotoxin was moderately correlated between family room and bedroom floor (r = 0.30), between family room and kitchen (r = 0.32), and between kitchen and bedroom (r = 0.42). Adjusting for season, the correlation of endotoxin levels within homes over time was 0.65 for both the bedroom and kitchen and 0.54 for the family room. The temporal within-home variance of endotoxin was lowest for bedroom floor samples and highest for kitchen samples. Between-home variance was lowest in the family room and highest for kitchen samples. Adjusting for season, within-home variation was less than between-home variation for all three rooms. These results suggest that room-to-room and home-to-home differences in endotoxin influence the total variability more than factors affecting endotoxin levels within a room over time

Cellular O-Glycome Reporter/Amplification to explore O-glycans of living cells

Protein O-glycosylation has key roles in many biological processes, but the repertoire of O-glycans synthesized by cells is difficult to determine. Here we describe an approach termed Cellular O-Glycome Reporter/Amplification (CORA), a sensitive method used to amplify and profile mucin-type O-glycans synthesized by living cells. Cells convert added peracetylated benzyl-α-N-acetylgalactosamine to a large variety of modified O-glycan derivatives that are secreted from cells, allowing for easy purification for analysis by HPLC and mass spectrometry (MS). Relative to conventional O-glycan analyses, CORA resulted in an ∼100-1,000-fold increase in sensitivity and identified a more complex repertoire of O-glycans in more than a dozen cell types from Homo sapiens and Mus musculus. Furthermore, when coupled with computational modeling, CORA can be used for predictions about the diversity of the human O-glycome and offers new opportunities to identify novel glycan biomarkers for human diseases

Registro geológico holocênico de recifes submersos na plataforma de Abrolhos

Sistemas recifais são feições comuns ao longo de muitos litorais tropicais modernos e as evidências de suas ocorrências durante diferentes níveis do mar têm sido muito estudadas por todo o mundo. A Plataforma de Abrolhos (Brasil) engloba o mais importante sistema de recifes de coral no Atlântico Sul apresentando altos níveis de endemismo, baixa diversidade e morfologia única de crescimento (formações recifais conhecidas como "chapeirões"). O desenvolvimento recifal na plataforma se dá pela presença de dois arcos recifais rasos paralelos à costa, além de recifes afogados ao longo das porções centro-norte e sul da mesma. O principal objetivo deste estudo é investigar o registro geológico do processo evolutivo holocênico de dois ambientes recifais na região da Plataforma de Abrolhos. Para isso foram sondados dois recifes submersos com seus topos a 4 e 15 metros de profundidade, chamados respectivamente de recifes “Shallow Water” (SW) e “Deep Water” (DW), onde foram coletados oito testemunhos distribuídos nas direções vertical (a partir do topo recifal) e horizontal (perpendiculares às paredes do recife). Os testemunhos coletados tiveram recuperação variando entre 0,70 e 2,03 metros de comprimento. Foram observadas significativas formações de corais e de algas (espécies de corais como Mussismilia harttii., Millepora sp., Siderastrea sp., Porites sp., Favia sp. e Madracis sp.; e espécies de algas calcárias como Hydrolithon sp., Lithophyllum kotschyanum, Lithophyllum sp., Amphiroa sp., Mesophyllum erubescens e Sporolithon episporum). No entanto, verificou-se que os organismos mais abundantes na edificação recifal foram os briozoários da família Schizoporellidae. Grandes formações de briozoários incrustantes foram descritas em todos os testemunhos, compreendendo cobertura superior a 15% na superfície longitudinal dos mesmos e podendo alcançar cobertura de até 52,9% em dois deles. Porém, briozoários foram mais representativos nos testemunhos do recife “DW”. Contraditoriamente, a fraca concorrência espacial identificada nos briozoários incrustantes pode ser a explicação para a maior ocorrência destes organismos nos testemunhos do recife “DW”, onde organismos zooxantelados e fortes concorrentes espaciais em ambiente com grande incidência de radiação solar não conseguem se desenvolver. Datações utilizando radiocarbono evidenciaram que o recife “DW” é mais antigo que o recife “SW”, bem como um maior acúmulo recifal (e principalmente no topo recifal) no recife “SW” nos últimos mil anos. Pela presença de corais zooxantelados e algas características de ambientes rasos, sugere-se que toda a estrutura recifal analisada se desenvolveu em ambiente de baixa profundidade (< 30 metros) na plataforma continental.Coral Reef systems are common features throughout many modern tropical coastal zone and the evidence of their occurrence during different sea levels have been studied worldwide. The Abrolhos Shelf (Brazil) encompasses the most important coral reef system in the South Atlantic showing high levels of endemism, low diversity and unique growth (locally known as “chapeirões”). The reef system along the shelf is characterized by shallow reef arcs parallel to the coast, and “give-up” reefs throughout the north-central and southern shelf. The main objective of this study is to investigate the geological record showing the Late Holocene evolution of two submerged pinnacles in the Abrolhos Shelf. Herein, two submerged pinnacles were drilledwith their tops at 4 and 15 m below sea level, called "Shallow Water Reef" (SW) and "Deep Water Reef" (DW). A total of eight cores were collected. Vertical (top down) and horizontal (perpendicular to the pinnacle wall) cores were recovered, ranging from 0.70 to 2.03 m in length. Significant coralgal framework was observed in the cores (corals such species as Mussismilia harttii., Millepora sp., Siderastrea sp., Porites sp., Favia sp. and Madracis sp.; and coralline algae such species as Hydrolithon sp., Lithophyllum kotschyanum, Lithophyllum sp., Amphiroa sp., Mesophyllum erubescens and Sporolithon episporum). However, we found that by far the most abundant framebuilding component were Schizoporellidae bryozoans. Extensive encrusting bryozoans were identified in all cores comprising between 15 - 52,9% of 2D areas. Bryozoans were most representative in the "DW" cores. Paradoxically, the poor spatial competition of encrusting bryozoans must be the explanation for the bryozoan dominance in the "DW" cores, which hermatypic corals can not develop. Radiocarbon dates indicate that the "DW" reef is older than the "SW" reef as well as higher reef accumulation rates occur in the past thousand years is in the "SW" reef. The results show that the both reefs have been developed in a shallow shelf environment (<30 meters depth

Inflation on the Brane with Vanishing Gravity

Many existing models of brane inflation suffer from a steep irreducible gravitational potential between the branes that causes inflation to end too early. Inspired by the fact that point masses in 2+1 D exert no gravitational force, we propose a novel unwarped and non-supersymmetric setup for inflation, consisting of 3-branes in two extra dimensions compactified on a sphere. The size of the sphere is stabilized by a combination of a bulk cosmological constant and a magnetic flux. Computing the 4D effective potential between probe branes in this background, we find a non-zero contribution only from exchange of level-1 KK modes of the graviton and radion. Identifying antipodal points on the 2-sphere projects out these modes, eliminating entirely the troublesome gravitational contribution to the inflationary potential.Comment: 19 pages, 11 figures, JHEP forma

Geogenetic patterns in mouse lemurs (genus Microcebus) reveal the ghosts of Madagascar's forests past.

Phylogeographic analysis can be described as the study of the geological and climatological processes that have produced contemporary geographic distributions of populations and species. Here, we attempt to understand how the dynamic process of landscape change on Madagascar has shaped the distribution of a targeted clade of mouse lemurs (genus Microcebus) and, conversely, how phylogenetic and population genetic patterns in these small primates can reciprocally advance our understanding of Madagascar's prehuman environment. The degree to which human activity has impacted the natural plant communities of Madagascar is of critical and enduring interest. Today, the eastern rainforests are separated from the dry deciduous forests of the west by a large expanse of presumed anthropogenic grassland savanna, dominated by the Family Poaceae, that blankets most of the Central Highlands. Although there is firm consensus that anthropogenic activities have transformed the original vegetation through agricultural and pastoral practices, the degree to which closed-canopy forest extended from the east to the west remains debated. Phylogenetic and population genetic patterns in a five-species clade of mouse lemurs suggest that longitudinal dispersal across the island was readily achieved throughout the Pleistocene, apparently ending at ∼55 ka. By examining patterns of both inter- and intraspecific genetic diversity in mouse lemur species found in the eastern, western, and Central Highland zones, we conclude that the natural environment of the Central Highlands would have been mosaic, consisting of a matrix of wooded savanna that formed a transitional zone between the extremes of humid eastern and dry western forest types

Identification of novel candidate target genes, including EPHB3, MASP1 and SST at 3q26.2–q29 in squamous cell carcinoma of the lung

<p>Abstract</p> <p>Background</p> <p>The underlying genetic alterations for squamous cell carcinoma (SCC) and adenocarcinoma (AC) carcinogenesis are largely unknown.</p> <p>Methods</p> <p>High-resolution array- CGH was performed to identify the differences in the patterns of genomic imbalances between SCC and AC of non-small cell lung cancer (NSCLC).</p> <p>Results</p> <p>On a genome-wide profile, SCCs showed higher frequency of gains than ACs (<it>p </it>= 0.067). More specifically, statistically significant differences were observed across the histologic subtypes for gains at 2q14.2, 3q26.2–q29, 12p13.2–p13.33, and 19p13.3, as well as losses at 3p26.2–p26.3, 16p13.11, and 17p11.2 in SCC, and gains at 7q22.1 and losses at 15q22.2–q25.2 occurred in AC (<it>P </it>< 0.05). The most striking difference between SCC and AC was gains at the 3q26.2–q29, occurring in 86% (19/22) of SCCs, but in only 21% (3/14) of ACs. Many significant genes at the 3q26.2–q29 regions previously linked to a specific histology, such as EVI1,<it>MDS1, PIK3CA </it>and <it>TP73L</it>, were observed in SCC (<it>P </it>< 0.05). In addition, we identified the following possible target genes (> 30% of patients) at 3q26.2–q29: <it>LOC389174 </it>(3q26.2),<it>KCNMB3 </it>(3q26.32),<it>EPHB3 </it>(3q27.1), <it>MASP1 </it>and <it>SST </it>(3q27.3), <it>LPP </it>and <it>FGF12 </it>(3q28), and <it>OPA1</it>,<it>KIAA022</it>,<it>LOC220729</it>, <it>LOC440996</it>,<it>LOC440997</it>, and <it>LOC440998 </it>(3q29), all of which were significantly targeted in SCC (<it>P </it>< 0.05). Among these same genes, high-level amplifications were detected for the gene, <it>EPHB3</it>, at 3q27.1, and <it>MASP1 </it>and <it>SST</it>, at 3q27.3 (18, 18, and 14%, respectively). Quantitative real time PCR demonstrated array CGH detected potential candidate genes that were over expressed in SCCs.</p> <p>Conclusion</p> <p>Using whole-genome array CGH, we have successfully identified significant differences and unique information of chromosomal signatures prevalent between the SCC and AC subtypes of NSCLC. The newly identified candidate target genes may prove to be highly attractive candidate molecular markers for the classification of NSCLC histologic subtypes, and could potentially contribute to the pathogenesis of the squamous cell carcinoma of the lung.</p

The Classical Harmonic Vibrations of the Atomic Centers of Mass with Micro Amplitudes and Low Frequencies Monitored by the Entanglement between the Two Two-level Atoms in a Single mode Cavity

We study the entanglement dynamics of the two two-level atoms coupling with a single-mode polarized cavity field after incorporating the atomic centers of mass classical harmonic vibrations with micro amplitudes and low frequencies. We propose a quantitative vibrant factor to modify the concurrence of the two atoms states. When the vibrant frequencies are very low, we obtain that: (i) the factor depends on the relative vibrant displacements and the initial phases rather than the absolute amplitudes, and reduces the concurrence to three orders of magnitude; (ii) the concurrence increases with the increase of the initial phases; (iii) the frequency of the harmonic vibration can be obtained by measuring the maximal value of the concurrence during a small time. These results indicate that even the extremely weak classical harmonic vibrations can be monitored by the entanglement of quantum states.Comment: 10 pages, 3 figure

Bottom mixed layer oxygen dynamics in the Celtic Sea

The seasonally stratified continental shelf seas are highly productive, economically important environments which are under considerable pressure from human activity. Global dissolved oxygen concentrations have shown rapid reductions in response to anthropogenic forcing since at least the middle of the twentieth century. Oxygen consumption is at the same time linked to the cycling of atmospheric carbon, with oxygen being a proxy for carbon remineralisation and the release of CO2. In the seasonally stratified seas the bottom mixed layer (BML) is partially isolated from the atmosphere and is thus controlled by interplay between oxygen consumption processes, vertical and horizontal advection. Oxygen consumption rates can be both spatially and temporally dynamic, but these dynamics are often missed with incubation based techniques. Here we adopt a Bayesian approach to determining total BML oxygen consumption rates from a high resolution oxygen time-series. This incorporates both our knowledge and our uncertainty of the various processes which control the oxygen inventory. Total BML rates integrate both processes in the water column and at the sediment interface. These observations span the stratified period of the Celtic Sea and across both sandy and muddy sediment types. We show how horizontal advection, tidal forcing and vertical mixing together control the bottom mixed layer oxygen concentrations at various times over the stratified period. Our muddy-sand site shows cyclic spring-neap mediated changes in oxygen consumption driven by the frequent resuspension or ventilation of the seabed. We see evidence for prolonged periods of increased vertical mixing which provide the ventilation necessary to support the high rates of consumption observed

Intrusion detection routers: Design, implementation and evaluation using an experimental testbed

In this paper, we present the design, the implementation details, and the evaluation results of an intrusion detection and defense system for distributed denial-of-service (DDoS) attack. The evaluation is conducted using an experimental testbed. The system, known as intrusion detection router (IDR), is deployed on network routers to perform online detection on any DDoS attack event, and then react with defense mechanisms to mitigate the attack. The testbed is built up by a cluster of sufficient number of Linux machines to mimic a portion of the Internet. Using the testbed, we conduct real experiments to evaluate the IDR system and demonstrate that IDR is effective in protecting the network from various DDoS attacks. © 2006 IEEE.published_or_final_versio