1,105 research outputs found
A survey of the South African shore-angling fishery
Roving creel surveys and aerial surveys of shore-angling were undertaken as part of a national investigation into linefishing in South Africa. Shore patrols utilized a random stratified sampling procedure to collect catchand-effort data, and a questionnaire provided information on fishing effort, angler demographics, economics and attitudes towards current regulations. A total of 1 677 patrols, covering 19 616 km, was conducted betweenApril 1994 and February 1996, during which period 9 523 anglers had their catches checked and 4 490 were interviewed. A further 16 497 km were covered by aerial surveys, when 22 609 anglers were counted. Fromthe aerial surveys, angler densities were highest on the KwaZulu-Natal coast (4.65 anglers.km–1), followed by the Southern Cape coast (2.29 anglers.km–1), the Eastern Cape coast (0.36 anglers.km–1) and the WestCoast (0.12 anglers.km–1). Catch rates varied from 1.5 kg.angler –1.day–1 on the Southern Cape coast to 0.45 kg.angler–1.day–1 on the KwaZulu-Natal coast. Total effort was estimated at 3.2×106 angler days.year–1 and the total catch was estimated at 4.5×106 fish.year–1 or 3 000 tons.year–1. Targeted species varied regionally, with elf Pomatomus saltatrix (29%) being the most sought after species on the KwaZulu-Natal coast, kob Argyrosomus japonicus (18%) on the Eastern Cape coast and galjoen Dichistius capensis on the Southern Cape coast (30%) and along the West Coast (50%). The catch composition by mass was dominated byP. saltatrix on the KwaZulu-Natal coast (29%), the Eastern Cape coast (26%), and on the Southern Cape coast (56%) and by white stumpnose Rhabdosargus globiceps (40%) on the West Coast. Although anglersgenerally supported the regulations currently governing the linefishery, the questionnaire results showed that knowledge and compliance of them was poor. A low level of law enforcement was found, except for inKwaZulu-Natal. Management of the linefishery is discussed in relation to the findings of this study
An economic valuation of the South African linefishery
Economic data collected during 1995 and 1996 in a national survey of shore-anglers and skiboat fishermen is used to provide an economic assessment of aspects of recreational, subsistence and commercial linefishing on the South African coastline. Results show that more than 90% of shore-anglers have incomes which lie in the highest two quintiles of the distribution of incomes, and tha
The structure of the PapD-PapGII pilin complex reveals an open and flexible P5 pocket
P pili are hairlike polymeric structures that mediate binding of uropathogenic Escherichia coli to the surface of the kidney via the PapG adhesin at their tips. PapG is composed of two domains: a lectin domain at the tip of the pilus followed by a pilin domain that comprises the initial polymerizing subunit of the 1,000-plus-subunit heteropolymeric pilus fiber. Prior to assembly, periplasmic pilin domains bind to a chaperone, PapD. PapD mediates donor strand complementation, in which a beta strand of PapD temporarily completes the pilin domain's fold, preventing premature, nonproductive interactions with other pilin subunits and facilitating subunit folding. Chaperone-subunit complexes are delivered to the outer membrane usher where donor strand exchange (DSE) replaces PapD's donated beta strand with an amino-terminal extension on the next incoming pilin subunit. This occurs via a zip-in-zip-out mechanism that initiates at a relatively accessible hydrophobic space termed the P5 pocket on the terminally incorporated pilus subunit. Here, we solve the structure of PapD in complex with the pilin domain of isoform II of PapG (PapGIIp). Our data revealed that PapGIIp adopts an immunoglobulin fold with a missing seventh strand, complemented in parallel by the G1 PapD strand, typical of pilin subunits. Comparisons with other chaperone-pilin complexes indicated that the interactive surfaces are highly conserved. Interestingly, the PapGIIp P5 pocket was in an open conformation, which, as molecular dynamics simulations revealed, switches between an open and a closed conformation due to the flexibility of the surrounding loops. Our study reveals the structural details of the DSE mechanism
Ground state cooling in a bad cavity
We study the mechanical effects of light on an atom trapped in a harmonic
potential when an atomic dipole transition is driven by a laser and it is
strongly coupled to a mode of an optical resonator. We investigate the cooling
dynamics in the bad cavity limit, focussing on the case in which the effective
transition linewidth is smaller than the trap frequency, hence when sideband
cooling could be implemented. We show that quantum correlations between the
mechanical actions of laser and cavity field can lead to an enhancement of the
cooling efficiency with respect to sideband cooling. Such interference effects
are found when the resonator losses prevail over spontaneous decay and over the
rates of the coherent processes characterizing the dynamics.Comment: 6 pages, 5 figures; J. Mod. Opt. (2007
Cavity QED with a Bose-Einstein condensate
Cavity quantum electrodynamics (cavity QED) describes the coherent
interaction between matter and an electromagnetic field confined within a
resonator structure, and is providing a useful platform for developing concepts
in quantum information processing. By using high-quality resonators, a strong
coupling regime can be reached experimentally in which atoms coherently
exchange a photon with a single light-field mode many times before dissipation
sets in. This has led to fundamental studies with both microwave and optical
resonators. To meet the challenges posed by quantum state engineering and
quantum information processing, recent experiments have focused on laser
cooling and trapping of atoms inside an optical cavity. However, the tremendous
degree of control over atomic gases achieved with Bose-Einstein condensation
has so far not been used for cavity QED. Here we achieve the strong coupling of
a Bose-Einstein condensate to the quantized field of an ultrahigh-finesse
optical cavity and present a measurement of its eigenenergy spectrum. This is a
conceptually new regime of cavity QED, in which all atoms occupy a single mode
of a matter-wave field and couple identically to the light field, sharing a
single excitation. This opens possibilities ranging from quantum communication
to a wealth of new phenomena that can be expected in the many-body physics of
quantum gases with cavity-mediated interactions.Comment: 6 pages, 4 figures; version accepted for publication in Nature;
updated Fig. 4; changed atom numbers due to new calibratio
Characterization of growth and metabolism of the haloalkaliphile Natronomonas pharaonis
Natronomonas pharaonis is an archaeon adapted to two extreme conditions: high salt concentration and alkaline pH. It has become one of the model organisms for the study of extremophilic life. Here, we present a genome-scale, manually curated metabolic reconstruction for the microorganism. The reconstruction itself represents a knowledge base of the haloalkaliphile's metabolism and, as such, would greatly assist further investigations on archaeal pathways. In addition, we experimentally determined several parameters relevant to growth, including a characterization of the biomass composition and a quantification of carbon and oxygen consumption. Using the metabolic reconstruction and the experimental data, we formulated a constraints-based model which we used to analyze the behavior of the archaeon when grown on a single carbon source. Results of the analysis include the finding that Natronomonas pharaonis, when grown aerobically on acetate, uses a carbon to oxygen consumption ratio that is theoretically near-optimal with respect to growth and energy production. This supports the hypothesis that, under simple conditions, the microorganism optimizes its metabolism with respect to the two objectives. We also found that the archaeon has a very low carbon efficiency of only about 35%. This inefficiency is probably due to a very low P/O ratio as well as to the other difficulties posed by its extreme environment
CP violation Beyond the MSSM: Baryogenesis and Electric Dipole Moments
We study electroweak baryogenesis and electric dipole moments in the presence
of the two leading-order, non-renormalizable operators in the Higgs sector of
the MSSM. Significant qualitative and quantitative differences from MSSM
baryogenesis arise due to the presence of new CP-violating phases and to the
relaxation of constraints on the supersymmetric spectrum (in particular, both
stops can be light). We find: (1) spontaneous baryogenesis, driven by a change
in the phase of the Higgs vevs across the bubble wall, becomes possible; (2)
the top and stop CP-violating sources can become effective; (3) baryogenesis is
viable in larger parts of parameter space, alleviating the well-known
fine-tuning associated with MSSM baryogenesis. Nevertheless, electric dipole
moments should be measured if experimental sensitivities are improved by about
one order of magnitude.Comment: 33 pages, 6 figure
Assimilating Seizure Dynamics
Observability of a dynamical system requires an understanding of its state—the collective values of its variables. However, existing techniques are too limited to measure all but a small fraction of the physical variables and parameters of neuronal networks. We constructed models of the biophysical properties of neuronal membrane, synaptic, and microenvironment dynamics, and incorporated them into a model-based predictor-controller framework from modern control theory. We demonstrate that it is now possible to meaningfully estimate the dynamics of small neuronal networks using as few as a single measured variable. Specifically, we assimilate noisy membrane potential measurements from individual hippocampal neurons to reconstruct the dynamics of networks of these cells, their extracellular microenvironment, and the activities of different neuronal types during seizures. We use reconstruction to account for unmeasured parts of the neuronal system, relating micro-domain metabolic processes to cellular excitability, and validate the reconstruction of cellular dynamical interactions against actual measurements. Data assimilation, the fusing of measurement with computational models, has significant potential to improve the way we observe and understand brain dynamics
Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy
We introduce UnaG as a green-to-dark photoswitching fluorescent protein capable of high-quality super-resolution imaging with photon numbers equivalent to the brightest photoswitchable red protein. UnaG only fluoresces upon binding of a fluorogenic metabolite, bilirubin, enabling UV-free reversible photoswitching with easily controllable kinetics and low background under Epi illumination. The on- and off-switching rates are controlled by the concentration of the ligand and the excitation light intensity, respectively, where the dissolved oxygen also promotes the off-switching. The photo-oxidation reaction mechanism of bilirubin in UnaG suggests that the lack of ligand-protein covalent bond allows the oxidized ligand to detach from the protein, emptying the binding cavity for rebinding to a fresh ligand molecule. We demonstrate super-resolution single-molecule localization imaging of various subcellular structures genetically encoded with UnaG, which enables facile labeling and simultaneous multicolor imaging of live cells. UnaG has the promise of becoming a default protein for high-performance super-resolution imaging. Photoconvertible proteins occupy two color channels thereby limiting multicolour localisation microscopy applications. Here the authors present UnaG, a new green-to-dark photoswitching fluorescent protein for super-resolution imaging, whose activation is based on a noncovalent binding with bilirubin
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