Epigenetic hereditary transcription profiles III, evidence for an epigenetic network resulting in gender, tissue and age-specific variation in overall transcription

<p>Abstract</p> <p>Background</p> <p>We have previously shown that deviations from the average transcription profile of a group of functionally related genes are not only heritable, but also demonstrate specific patterns associated with age, gender and differentiation, thereby implicating genome-wide nuclear programming as the cause. To determine whether these results could be reproduced, a different micro-array database (obtained from two types of muscle tissue, derived from 81 human donors aged between 16 to 89 years) was studied.</p> <p>Results</p> <p>This new database also revealed the existence of age, gender and tissue-specific features in a small group of functionally related genes. In order to further analyze this phenomenon, a method was developed for quantifying the contribution of different factors to the variability in gene expression, and for generating a database limited to residual values reflecting constitutional differences between individuals. These constitutional differences, presumably epigenetic in origin, contribute to about 50% of the observed residual variance which is connected with a network of interrelated changes in gene expression with some genes displaying a decrease or increase in residual variation with age.</p> <p>Conclusion</p> <p>Epigenetic variation in gene expression without a clear concomitant relation to gene function appears to be a widespread phenomenon. This variation is connected with interactions between genes, is gender and tissue specific and is related to cellular aging.</p> <p>This finding, together with the method developed for analysis, might contribute to the elucidation of the role of nuclear programming in differentiation, aging and carcinogenesis</p> <p>Reviewers</p> <p>This article was reviewed by Thiago M. Venancio (nominated by Aravind Iyer), Hua Li (nominated by Arcady Mushegian) and Arcady Mushegian and J.P.de Magelhaes (nominated by G. Church).</p

Hereditary profiles of disorderly transcription?

BACKGROUND: Microscopic examination of living cells often reveals that cells from some cell strains appear to be in a permanent state of disarray without obvious reason. In all probability such a disorderly state affects cell functioning. The aim of this study was to establish whether a disorderly state could occur that adversely affects gene expression profiles and whether such a state might have biomedical consequences. To this end, the expression profiles of the 14 genes of the proteasome derived from the GEO SAGE database were utilized as a model system. RESULTS: By adopting the overall expression profile as the standard for normal expression, deviation in transcription was frequently observed. Each deviating tissue exhibited its own characteristic profile of over-expressed and under-expressed genes. Moreover such a specific deviating profile appeared to be epigenetic in origin and could be stably transmitted to a clonal derivative e.g. from a precancerous normal tissue to its tumor. A significantly greater degree of deviation was observed in the expression profiles from the tumor tissues. The changes in the expression of different genes display a network of interdependencies. Therefore our hypothesis is that deviating profiles reflect disorder in the localization of genes within the nucleus The underlying cause(s) for these disorderly states remain obscure; it could be noise and/or deterministic chaos. Presence of mutational damage does not appear to be predominantly involved. CONCLUSION: As disturbances in expression profiles frequently occur and have biomedical consequences, its determination could prove of value in several fields of biomedical research. REVIEWERS: This article was reviewed by Trey Ideker, Itai Yanai and Stephan Bec

Nutrition and health claims – call for and justification of governmental intervention from the consumers’ perspective

In December 2006 the Regulation (EC) No. 1924/2006 on the use of nutrition and health claims (NHCs) on foods was enacted in order to prevent consumer deception and to harmonise law within the EU. Against this background, this paper analyses the potential costs and benefits linked with NHCs and the necessity for governmental intervention to regulate NHCs within a theoretical and empirical framework. The theoretical investigation shows that NHCs can induce direct economic effects as well as spillover effects in the market of information. Whether those effects are beneficial or adverse depends on the truthfulness of the NHCs, and consumers’ perception and processing of such claims. As self regulatory forces of the market might not be sufficient to prevent market failure due to fraudulent claims, governmental intervention seems necessary. An analysis of the EU Regulation on NHCs reveals that this law focuses on preventing the authorisation of false or misleading claims. It is less concerned with not authorising a true and correctly understood claim. The results of the empirical analysis which is based on a standardized consumer survey reveal that the stated impact of NHCs on product perception considerably differs among consumers. While e.g. some consumers feel misled by NHCs on products with a negative nutrient profile such as candies, others point out that such claims have no impact on their product perception or even help them to make better choices. The results also indicate that the great majority of consumers is opposed to a ban of NHCs on products with a negative nutrient profile such as candies and salt.nutrition and health claims, consumer deception, information economics, market transparency, consumer protection policy, Consumer/Household Economics, Food Consumption/Nutrition/Food Safety,

Entwicklung regionaler Vermarktungsstrategien gefährdeter Tomatensorten in NRW, Deutschland

Four old and endangered tomato cultivars were cultivated in a greenhouse under organic conditions. With an average yield between 10 to 12 kg m-2, these old cultivars can compete with those tomato cultivars currently dominating the organic market. New marketing strategies were developed, tested in focus groups and offered on the re-gional market in organic shops and in a staff canteen. Both a coloured tomato basket and a mixed tomato fruit salad were positively evaluated by the consumers. Short distribution distances, preserving biodiversity and organic production were important preference factors for the consumers

Food Value Chain Coordination in Practice: European and Australian Case Studies of the Creation of Chain Good Innovations

Food value chain businesses form alliances with horizontal and/or vertical partners to take collective action to either overcome or ameliorate chain failure, or to take advantage of new opportunities available due to innovations in products or processes. The desired outcomes from the collective action would not be possible to achieve if these businesses acted independently. While such alliances may take many forms, depending on degree of commitment and infrastructure linkages, they can often be considered to be clubs. Four such types of clubs can be identified (1) horizontal clubs comprising businesses that take collective action across a single cross-section or an aggregate of multiple cross-sections in the value chain; (2) vertical clubs, which consist of businesses that form a strategic alliance for collective action along a single value chain within a network of chains; (3) clubs that specialise in a single product or multiple products in the value chain; or (4) clubs focusing on a single input/activity or multiple inputs/activities. Thus the path to collective action chosen by clubs may vary according to existing capabilities and the scope for collaboration, particularly in relation to the potential for value-creating innovation. The result of the collective action is the provision of a chain good or service which usually leads to greater and more valuable chain coordination. By collectively identifying, funding and acting to capture positive externalities associated with innovation, businesses in many parts of a food value chain can widen opportunities to increase whole-of-chain surplus as well as increase private profits.In this paper four mini-case studies are presented which demonstrate the breadth of past collective actions that have been undertaken by a substantial proportion of businesses in food value chains, two in Europe and two in Australia. These are (1) the Euro Pool System, (2) Global Standards certification in Europe and globally, (3) Meat Standards Australia, and (4) the OBE Beef organic producer alliance in Australia. Each case study yields insights into the rationale of how businesses in different food value chains in different countries have acted as a club to use their joint resources to internalise positive innovation and coordination externalities that would not have been possible to achieve were these businesses to act independently

Food Value Chain Coordination in Practice: European and Australian Case Studies of the Creation of Chain Good Innovations

Food value chain businesses form alliances with horizontal and/or vertical partners to take collective action to either overcome or ameliorate chain failure, or to take advantage of new opportunities available due to innovations in products or processes. The desired outcomes from the collective action would no t be possible to achieve if these businesses acted independently. While such alliances and collaborations may take many forms, depending on the degree of commitment, the kind of governance and infrastructure linkages, they can often be thought of as “clubs ” for the purpose of economic analysis. Several different types of clubs can be identified, thus the path to collective action chosen by clubs may vary according to existing capabilities and the scope for collaboration, particularly in relation to the potential for value-creating innovation. The result of the collective action is the provision of a chain good or service, which usually leads to greater and more valuable chain coordination. By collectively identifying, funding and acting to capture positive externalities associated with innovation, businesses in many parts of a food value chain can widen opportunities to increase whole-of-chain surplus as well as private profits. In this paper five mini-case studies are presented to demonstrate the breadth of past collective actions undertaken by businesses in food value chains, two in Europe and three in Australia. These are the Euro Pool System, and Global Standards certification in Europe and globally, as well as Meat Standards Australia, an Australian beef organic producer alliance (OBE Organic®), and the supply of food to households during Covid-19 lockdown in Australia. Each case study yields insights into the rationale of how businesses in different food value chains in different countries have acted as a club to use their joint resources to internalise positive innovation and coordination externalities

F-18-fluorodeoxyglucose positron emission tomography combined with CT in critically ill patients with suspected infection

Contains fulltext : 88623.pdf (publisher's version ) (Closed access)PURPOSE: To assess the value of F-18-fluorodeoxyglucose positron emission tomography (FDG-PET) combined with CT in critically ill patients suspected of having an infection. METHODS: FDG-PET CT scans requested for evaluation of a suspected infection or inflammatory process in critically ill, mechanically ventilated patients were analyzed (blinded for the final clinical diagnosis) and compared with clinical follow-up. RESULTS: Thirty-five FDG-PET/CT scans performed in 33 ICU patients (28 adults and 5 children), median age 58 years (range 1 month-72 years), were analyzed. Twenty-one FDG-PET/CT scans were true positive. Three FDG-PET/CT scans were considered false positive, in one case leading to additional diagnostic procedures (specificity 79%). Additionally, 11 true negatives were found (sensitivity 100%), leading to an overall accuracy of 91%. CONCLUSIONS: FDG-PET/CT scanning is of additional value in the evaluation of suspected infection in critically ill patients in whom conventional diagnostics did not lead to a diagnosis. Apart from the high accuracy, in this study it appeared that, in addition to conventional diagnostic techniques that were routinely performed, a normal FDG-PET/CT ruled out important infections requiring prolonged antibiotic therapy or drainage. Since sensitivity is lower in highly metabolic active tissues (e.g., endocarditis, meningitis), the FDG-PET/CT scan is not suited to detect infections in these tissues.01 maart 201

Wie Unternehmen des Baugewerbes die Corona-Krise bewerten

Die wirtschaftliche Situation der Unternehmen stellt sich im Baugewerbe besser dar als in den anderen Industrien. Gleichzeitig haben die Unternehmen des Baugewerbes weniger staatliche Maßnahmen in Anspruch genommen. Zur Kompensation der Mehrbelastung greifen die meisten von ihnen auf eine Reduktion von Löhnen und Boni zurück. Innerhalb des Baugewerbes werden zwischen Ausbau- und Bauhauptgewerbe starke Unterschiede in der krisenbedingten Belastung deutlich. Dies betrifft die Erwartungen zur Umsatzentwicklung sowie die Inanspruchnahme staatlicher Maßnahmen

Влияние толщины диффузионного слоя на возникновение динамических неустойчивостей в модельном электрокаталитическом процессе

Определено влияние толщины диффузионного слоя Нернста на возникновение неустойчивостей Хопфа, седло–узел и гомоклинной неустойчивости в модельном электрокаталитическом процессе на поверхности сферического электрода при потенциостатических условиях. Показано, что увеличение толщины диффузионного слоя Нернста способстует расширению области потенциалов (в системе могут наблюдаться спонтанные периодические колебания тока), а также области бистабильности системы. Возможная реализация в системе гомоклинной бифуркации может привести и к более сложным динамическим режимам.Визначено вплив товщини дифузійного шару Нернста на виникнення нестійкості Хопфа, сідло–вузол та гомоклінної нестійкості в модельному електрокаталітичному процесі на поверхні сферичного електроду за потенціостатичних умов. Показано, що збільшення товщини дифузійного шару Нернста спричинює розширення області потенціалів (у системі можуть спостерігатися спонтанні періодичні коливання струму), а також області бістабільності системи. Можлива реалізація в системі гомоклінної біфуркації може привести і до більш складних динамічних режимів.The influence of the Nernst diffusion layer thickness on the appearance of the Hopf instability, saddle–node instability, and homoclinic instability in a model electrocatalytic process on a spherical electrode surface under potentiostatic conditions is determined. It is shown that the Nernst diffusion layer thickness increase leads to an increase in the potential range, where spontaneous periodic current oscillations can be observed and also in the system bistability range. A possible realization of the homoclinic bifurcation in the system can initiate more complicated dynamical regimes