Optical studies of transparent ferroelectric strontium-barium niobate/silica nanocomposite

Author name used in this publication: C. L. MakAuthor name used in this publication: K. H. Wong2003-2004 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Non-invasive molecular imaging of inflammatory macrophages in allograft rejection.

BackgroundMacrophages represent a critical cell type in host defense, development and homeostasis. The ability to image non-invasively pro-inflammatory macrophage infiltrate into a transplanted organ may provide an additional tool for the monitoring of the immune response of the recipient against the donor graft. We therefore decided to image in vivo sialoadhesin (Sn, Siglec 1 or CD169) using anti-Sn mAb (SER-4) directly radiolabelled with (99m)Tc pertechnetate.MethodsWe used a heterotopic heart transplantation model where allogeneic or syngeneic heart grafts were transplanted into the abdomen of recipients. In vivo nanosingle-photon emission computed tomography (SPECT/CT) imaging was performed 7 days post transplantation followed by biodistribution and histology.ResultsIn wild-type mice, the majority of (99m)Tc-SER-4 monoclonal antibody cleared from the blood with a half-life of 167 min and was located predominantly on Sn(+) tissues in the spleen, liver and bone marrow. The biodistribution in the transplantation experiments confirmed data derived from the non-invasive SPECT/CT images, with significantly higher levels of (99m)Tc-SER-4 observed in allogeneic grafts (9.4 (±2.7) %ID/g) compared to syngeneic grafts (4.3 (±10.3) %ID/g) (p = 0.0022) or in mice which received allogeneic grafts injected with (99m)Tc-IgG isotype control (5.9 (±0.6) %ID/g) (p = 0.0185). The transplanted heart to blood ratio was also significantly higher in recipients with allogeneic grafts receiving (99m)Tc-SER-4 as compared to recipients with syngeneic grafts (p = 0.000004) or recipients with allogeneic grafts receiving (99m)Tc-IgG isotype (p = 0.000002).ConclusionsHere, we demonstrate that imaging of Sn(+) macrophages in inflammation may provide an important additional and non-invasive tool for the monitoring of the pathophysiology of cellular immunity in a transplant model

Photoluminescence of transparent strontium–barium–niobate-doped silica nanocomposites

Author name used in this publication: C. L. MakAuthor name used in this publication: K. H. Wong2001-2002 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Effects of postdeposition annealing on the dielectric properties of antiferroelectric lanthanum-doped lead zirconate stannate titanate thin films derived from pulsed laser deposition

Author name used in this publication: K. H. Wong2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

High tunability in compositionally graded epitaxial barium strontium titanate thin films by pulsed-laser deposition

2002-2003 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Industry practices in project management for multimedia information systems

This paper describes ongoing research directed at formulating a set of appropriate measures for assessing and ultimately predicting effort requirements for multimedia systems development. Whilst significant advances have been made in the determination of measures for both transaction-based and process-intensive systems, very little work has been undertaken in relation to measures for multimedia systems. A small preliminary empirical study is reviewed as a precursor to a more exploratory investigation of the factors that are considered by industry to be influential in determining development effort. This work incorporates the development and use of a goal-based framework to assist the measure selection process from a literature basis, followed by an industry questionnaire. The results provide a number of preliminary but nevertheless useful insights into contemporary project management practices with respect to multimedia systems

Validity and reliability of the Chinese critical thinking disposition inventory

2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Tuning the Resonant Frequency and Damping of an Electromagnetic Energy Harvester Using Power Electronics

Accepted versio

Role of oxygen pressure during pulsed laser deposition on the electrical and dielectric properties of antiferroelectric lanthanum-doped lead zirconate stannate titanate thin films

Author name used in this publication: K. H. Wong2004-2005 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Site-specific incorporation of phosphotyrosine using an expanded genetic code.

Access to phosphoproteins with stoichiometric and site-specific phosphorylation status is key to understanding the role of protein phosphorylation. Here we report an efficient method to generate pure, active phosphotyrosine-containing proteins by genetically encoding a stable phosphotyrosine analog that is convertible to native phosphotyrosine. We demonstrate its general compatibility with proteins of various sizes, phosphotyrosine sites and functions, and reveal a possible role of tyrosine phosphorylation in negative regulation of ubiquitination