877 research outputs found

    Multiple circuit switch apparatus with improved pivot actuator structure Patent

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    Multiple circuit switch apparatus requiring minimum hand and eye movement by operato

    Management Strategies for Tomato Spotted Wilt Virus in Utah Tomatoes

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    Plant diseases are among the leading causes of tomato stand loss in the state of Utah. Viral plant diseases are often transferred from one plant to another by insect feeding. In Utah, one of the leading viruses that infects tomatoes is Tomato spotted wilt virus (TSWV) which is spread by thrips. The objectives of this study were as follows. Determine the impact from reflective mulch and row covers on thrips population numbers. Determine the effects of row covers on the TSWV infection rate. Compare varietal response to thrip populations. This 2-year study focused on the use of two different types of plastic mulch and the use of row covers to deter the number of virus-carrying insects on tomato plants. Silver reflective mulch and floating row covers were compared to black plastic mulch, which is the tomato industry standard. This study was set up in a randomized complete block design and repeated twice over 2 years. Two different tomato varieties, “ Mountain Glory” and “Celebrity,” were used in this study to determine if Mountain Glory was a viable option for tomato producers in the southern part of Utah. Plants that show signs of infection were sampled and tested for presence of the virus. Two-sided yellow sticky trap cards were deployed within the trial plots, collected three times each year, and analyzed for the number of thrips. The results showed that silver reflective mulch, and the use of floating row covers significantly reduced the number of thrips in tomato production compared to the standard black plastic mulch. No significant difference was observed when comparing the number of thrips present on the sticky cards when analyzed by tomato variet

    Microbial Biosafety of Pilot-scale Bioreactor Treating MTBE and TBA-contaminated Drinking Water Supply

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    A pilot-scale sand-based fluidized bed bioreactor (FBBR) was utilized to treat both methyl tert-butyl ether (MTBE) and tert-butyl alcohol (TBA) from a contaminated aquifer. To evaluate the potential for re-use of the treated water, we tested for a panel of water quality indicator microorganisms and potential waterborne pathogens including total coliforms, Escherichia coli, Salmonella and Shigella spp., Campylobacter jejuni, Aeromonas hydrophila, Legionella pneumophila, Vibrio cholerae, Yersinia enterocolytica and Mycobacterium avium in both influent and treated waters from the bioreactor. Total bacteria decreased during FBBR treatment. E. coli, Salmonella and Shigella spp., C. jejuni, V. cholerae, Y. enterocolytica and M. avium were not detected in aquifer water or bioreactor treated water samples. For those pathogens detected, including total coliforms, L. pneumophila and A. hydrophila, numbers were usually lower in treated water than influent samples, suggesting removal during treatment. The detection of particular bacterial species reflected their presence or absence in the influent waters

    Changes in soil microbial community structure influenced by agricultural management practices in a mediterranean agro-ecosystem.

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    Agricultural practices have proven to be unsuitable in many cases, causing considerable reductions in soil quality. Land management practices can provide solutions to this problem and contribute to get a sustainable agriculture model. The main objective of this work was to assess the effect of different agricultural management practices on soil microbial community structure (evaluated as abundance of phospholipid fatty acids, PLFA). Five different treatments were selected, based on the most common practices used by farmers in the study area (eastern Spain): residual herbicides, tillage, tillage with oats and oats straw mulching; these agricultural practices were evaluated against an abandoned land after farming and an adjacent long term wild forest coverage. The results showed a substantial level of differentiation in the microbial community structure, in terms of management practices, which was highly associated with soil organic matter content. Addition of oats straw led to a microbial community structure closer to wild forest coverage soil, associated with increases in organic carbon, microbial biomass and fungal abundances. The microbial community composition of the abandoned agricultural soil was characterised by increases in both fungal abundances and the metabolic quotient (soil respiration per unit of microbial biomass), suggesting an increase in the stability of organic carbon. The ratio of bacteria:fungi was higher in wild forest coverage and land abandoned systems, as well as in the soil treated with oat straw. The most intensively managed soils showed higher abundances of bacteria and actinobacteria. Thus, the application of organic matter, such as oats straw, appears to be a sustainable management practice that enhances organic carbon, microbial biomass and activity and fungal abundances, thereby changing the microbial community structure to one more similar to those observed in soils under wild forest coverage

    Gene \u3cem\u3emdpC\u3c/em\u3e Plays a Regulatory Role in the Methyl-\u3cem\u3etert\u3c/em\u3e-butyl Ether Degradation Pathway of \u3cem\u3eMethylibium petroleiphilum\u3c/em\u3e Strain PM1

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    Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC−. We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA
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