1,451 research outputs found
Field-induced domain wall propagation: beyond the one-dimensional model
We have investigated numerically the field-driven propagation of
perpendicularly magnetized ferromagnetic layers. It was then compared to the
historical one-dimensional domain wall (DW) propagation model widely used in
spintronics studies of magnetic nanostructures. In the particular regime of
layer thickness (h) of the order of the exchange length, anomalous velocity
peaks appear in the precessional regime, their shape and position shifting with
h. This has also been observed experimentally. Analyses of the simulations show
a distinct correlation between the curvature of the DW and the twist of the
magnetization vector within it, and the velocity peak. Associating a
phenomenological description of this twist with a four-coordinate DW
propagation model, we reproduce very well these kinks and show that they result
from the torque exerted by the stray field created by the domains on the
twisted magnetization. The position of the peaks is well predicted from the
DW's first flexural mode frequency, and depends strongly on the layer
thickness. Comparison of the proposed model to DW propagation data obtained on
dilute semiconductor ferromagnets GaMnAs and GaMnAsP sheds light on the origin
of the measured peaks
Geoglossomycetes cl. nov., Geoglossales ord. nov. and taxa above class rank in the Ascomycota Tree of Life
Featuring a high level of taxon sampling across Ascomycota, we evaluate a multi-gene phylogeny and propose a novel order and class in Ascomycota. We describe two new taxa, Geoglossomycetes and Geoglossales, to host three earth tongue genera: Geoglossum, Trichoglossum and Sarcoleotia as a lineage of ‘Leotiomyceta’. Correspondingly, we confirm that these genera are not closely related to the genera Neolecta, Mitrula, Cudonia, Microglossum, Thuemenidum, Spathularia and Bryoglossum, all of which have been previously placed within the Geoglossaceae. We also propose a non-hierarchical system for naming well-resolved nodes, such as ‘Saccharomyceta’, ‘Dothideomyceta’, and ‘Sordariomyceta’ for supraordinal nodes, within the current phylogeny, acting as rankless taxa. As part of this revision, the continued use of ‘Leotiomyceta’, now as a rankless taxon, is proposed
Analysis of resonance multipoles from polarization observables in eta photoproduction
A combined analysis of new eta photoproduction data for total and
differential cross sections, target asymmetry and photon asymmetry is
presented. Using a few reasonable assumptions we perform the first
model-independent analysis of the E0+, E2- and M2- eta photoproduction
multipoles. Making use of the well-known A3/2 helicity amplitude of the
D13(1520) state we extract its branching ratio to the eta-N channel,
Gamma(eta,N)/Gamma = (0.08 +- 0.01)%. At higher energies, we show that the
photon asymmetry is extremely sensitive to small multipoles that are excited by
photons in the helicity 3/2 state. The new GRAAL photon asymmetry data at
higher energy show a clear signal of the F15(1680) excitation which permits
extracting an F15(1680)->eta,N branching ratio of (0.15 +0.35 -0.10)%.Comment: 14 pages of LATEX including 7 postscript figure
Development and implementation of an Internet-based survivorship care program for cancer survivors treated with hematopoietic stem cell transplantation.
The Internet provides a widely accessible modality for meeting survivorship care needs of cancer survivors. In this paper, we describe the development and implementation of an Internet site designed as a base from which to conduct a randomized controlled trial to meet psycho-educational needs of hematopoietic stem cell transplantation (HSCT) survivors
Loss of AP-3 function affects spontaneous and evoked release at hippocampal mossy fiber synapses
Synaptic vesicle (SV) exocytosis mediating neurotransmitter release occurs
spontaneously at low intraterminal calcium concentrations and is stimulated by
a rise in intracellular calcium. Exocytosis is compensated for by the
reformation of vesicles at plasma membrane and endosomes. Although the adaptor
complex AP-3 was proposed to be involved in the formation of SVs from
endosomes, whether its function has an indirect effect on exocytosis remains
unknown. Using mocha mice, which are deficient in functional AP-3, we identify
an AP-3-dependent tetanus neurotoxin-resistant asynchronous release that can be
evoked at hippocampal mossy fiber (MF) synapses. Presynaptic targeting of the
tetanus neurotoxin-resistant vesicle soluble N-ethylmaleimide-sensitive factor
attachment protein receptor (SNARE) tetanus neurotoxin-insensitive
vesicle-associated membrane protein (TI-VAMP) is lost in mocha hippocampal MF
terminals, whereas the localization of synaptobrevin 2 is unaffected. In
addition, quantal release in mocha cultures is more frequent and more sensitive
to sucrose. We conclude that lack of AP-3 results in more constitutive
secretion and loss of an asynchronous evoked release component, suggesting an
important function of AP-3 in regulating SV exocytosis at MF terminals
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Looking beyond the exome: a phenotype-first approach to molecular diagnostic resolution in rare and undiagnosed diseases.
PurposeTo describe examples of missed pathogenic variants on whole-exome sequencing (WES) and the importance of deep phenotyping for further diagnostic testing.MethodsGuided by phenotypic information, three children with negative WES underwent targeted single-gene testing.ResultsIndividual 1 had a clinical diagnosis consistent with infantile systemic hyalinosis, although WES and a next-generation sequencing (NGS)-based ANTXR2 test were negative. Sanger sequencing of ANTXR2 revealed a homozygous single base pair insertion, previously missed by the WES variant caller software. Individual 2 had neurodevelopmental regression and cerebellar atrophy, with no diagnosis on WES. New clinical findings prompted Sanger sequencing and copy number testing of PLA2G6. A novel homozygous deletion of the noncoding exon 1 (not included in the WES capture kit) was detected, with extension into the promoter, confirming the clinical suspicion of infantile neuroaxonal dystrophy. Individual 3 had progressive ataxia, spasticity, and magnetic resonance image changes of vanishing white matter leukoencephalopathy. An NGS leukodystrophy gene panel and WES showed a heterozygous pathogenic variant in EIF2B5; no deletions/duplications were detected. Sanger sequencing of EIF2B5 showed a frameshift indel, probably missed owing to failure of alignment.ConclusionThese cases illustrate potential pitfalls of WES/NGS testing and the importance of phenotype-guided molecular testing in yielding diagnoses
Detection and Identification of Fungi Intimately Associated with the Brown Seaweed Fucus serratus
The filamentous fungi associated with healthy and decaying Fucus serratus thalli were studied over a 1-year period using isolation methods and molecular techniques such as 28S rRNA gene PCR-denaturing gradient gel electrophoresis (DGGE) and phylogenetic and real-time PCR analyses. The predominant DGGE bands obtained from healthy algal thalli belonged to the Lindra, Lulworthia, Engyodontium, Sigmoidea/Corollospora complex, and Emericellopsis/Acremonium-like ribotypes. In the culture-based analysis the incidence of recovery was highest for Sigmoidea marina isolates. In general, the environmental sequences retrieved could be matched unambiguously to isolates recovered from the seaweed except for the Emericellopsis/Acremonium-like ribotype, which showed 99% homology with the sequences of four different isolates, including that of Acremonium fuci. To estimate the extent of colonization of A. fuci, we used a TaqMan real-time quantitative PCR assay for intron 3 of the beta-tubulin gene, the probe for which proved to be species specific even when it was used in amplifications with high background concentrations of other eukaryotic DNAs. The A. fuci sequence was detected with both healthy and decaying thalli, but the signal was stronger for the latter. Additional sequence types, representing members from the Dothideomycetes, were recovered from the decaying thallus DNA, which suggested that a change in fungal community structure had occurred. Phylogenetic analysis of these environmental sequences and the sequences of isolates and type species indicated that the environmental sequences were novel in the Dothideomycetes
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