2,583 research outputs found
Dissection of a novel molecular determinant mediating Golgi to trans -Golgi network transition
Abstract.: Two major functions of the Golgi apparatus (GA) are formation of complex glycans and sorting of proteins destined for various subcellular compartments or secretion. To fulfill these tasks proper localization of the accessory proteins within the different sub-compartments of the GA is crucial. Here we investigate structural determinants mediating transition of the two glycosyltransferases ÎČ-1,4- galactosyltransferase 1 (gal-T1) and the α-1,3-fucosyltransferase 6 (fuc-T6) from the trans-Golgi cisterna to the trans-Golgi network (TGN). Upon treatment with the ionophore monensin both glycosyltransferases are found in TGN-derived swollen vesicles, as determined by confocal fluorescence microscopy and density gradient fractionation. Both enzymes carry a signal consisting of the amino acids E5P6 in gal-T1 and D2P3 in fuc-T6 necessary for the transition of these glycosyltransferases from the trans-Golgi cisterna to the TGN, but not for their steady state localization in the trans-Golgi cistern
Binary Central Stars of Planetary Nebulae Discovered Through Photometric Variability III: The Central Star of Abell 65
A growing number of close binary stars are being discovered among central
stars of planetary nebulae. Recent and ongoing surveys are finding new systems
and contributing to our knowledge of the evolution of close binary systems. The
push to find more systems was largely based on early discoveries which
suggested that 10 to 15% of all central stars are close binaries. One goal of
this series of papers is confirmation and classification of these systems as
close binaries and determination of binary system parameters. Here we provide
time-resolved multi-wavelength photometry of the central star of Abell 65 as
well as further analysis of the nebula and discussion of possible
binary--nebula connections. Our results for Abell 65 confirm recent work
showing that it has a close, cool binary companion, though several of our model
parameters disagree with the recently published values. With our longer time
baseline of photometric observations from 1989--2009 we also provide a more
precise orbital period of 1.0037577 days.Comment: Accepted for publication in the Astronomical Journa
Sublimation of Li@C60
R.S. acknowledges financial support from the Scottish Funding Council thorugh SRD-Grant (HR07003) and H.J.C. from an EPSRC DTG studentship (EP/M508214/1).Experiments that probe the fundamental properties of endohedral fullerenes often require the preparation of molecular beams or thin films of the neutral molecules. It is challenging to cleanly sublime this class of molecules without producing some thermal degradation. We report combined gas phase and scanning tunnelling microscopy studies that probe the thermal decay of commercial [Li+C60]PF6- in a quartz ampoule and provide treatment conditions that will allow the sublimation of intact, neutral Li@C60 accompanied by a well-characterised component of neutral C60. The decay of the material at appropriate temperatures can be modelled with the assumption of a second order decay process in the oven yielding Arrhenius parameters that can predict the ratio of Li@C60 to C60 in the sublimed material.Publisher PDFPeer reviewe
Surface structure of i-Al(68)Pd(23)Mn(9): An analysis based on the T*(2F) tiling decorated by Bergman polytopes
A Fibonacci-like terrace structure along a 5fold axis of i-Al(68)Pd(23)Mn(9)
monograins has been observed by T.M. Schaub et al. with scanning tunnelling
microscopy (STM). In the planes of the terraces they see patterns of dark
pentagonal holes. These holes are well oriented both within and among terraces.
In one of 11 planes Schaub et al. obtain the autocorrelation function of the
hole pattern. We interpret these experimental findings in terms of the
Katz-Gratias-de Boisseu-Elser model. Following the suggestion of Elser that the
Bergman clusters are the dominant motive of this model, we decorate the tiling
T*(2F) by the Bergman polytopes only. The tiling T*(2F) allows us to use the
powerful tools of the projection techniques. The Bergman polytopes can be
easily replaced by the Mackay polytopes as the decoration objects. We derive a
picture of ``geared'' layers of Bergman polytopes from the projection
techniques as well as from a huge patch. Under the assumption that no surface
reconstruction takes place, this picture explains the Fibonacci-sequence of the
step heights as well as the related structure in the terraces qualitatively and
to certain extent even quantitatively. Furthermore, this layer-picture requires
that the polytopes are cut in order to allow for the observed step heights. We
conclude that Bergman or Mackay clusters have to be considered as geometric
building blocks of the i-AlPdMn structure rather than as energetically stable
entities
"It's stressful having all these phones": {I}nvestigating Sex Workers' Safety Goals, Risks, and Practices Online
Anaesthetics and cardiac preconditioning. Part I. Signalling and cytoprotective mechanisms
Cardiac preconditioning represents the most potent and consistently reproducible method of rescuing heart tissue from undergoing irreversible ischaemic damage. Major milestones regarding the elucidation of this phenomenon have been passed in the last two decades. The signalling and amplification cascades from the preconditioning stimulus, be it ischaemic or pharmacological, to the putative endâeffectors, including the mechanisms involved in cellular protection, are discussed in this review. Volatile anaesthetics and opioids effectively elicit pharmacological preconditioning. Anaestheticâinduced preconditioning and ischaemic preconditioning share many fundamental steps, including activation of Gâproteinâcoupled receptors, multiple protein kinases and ATPâsensitive potassium channels (KATP channels). Volatile anaesthetics prime the activation of the sarcolemmal and mitochondrial KATP channels, the putative endâeffectors of preconditioning, by stimulation of adenosine receptors and subsequent activation of protein kinase C (PKC) and by increased formation of nitric oxide and free oxygen radicals. In the case of desflurane, stimulation of αâ and ÎČâadrenergic receptors may also be of importance. Similarly, opioids activate ÎŽâ and Îșâopioid receptors, and this also leads to PKC activation. Activated PKC acts as an amplifier of the preconditioning stimulus and stabilizes, by phosphorylation, the open state of the mitochondrial KATP channel (the main endâeffector in anaesthetic preconditioning) and the sarcolemmal KATP channel. The opening of KATP channels ultimately elicits cytoprotection by decreasing cytosolic and mitochondrial Ca2+ overload. Br J Anaesth 2003; 91: 551-6
Encoding dynamics for multiscale community detection: Markov time sweeping for the Map equation
The detection of community structure in networks is intimately related to
finding a concise description of the network in terms of its modules. This
notion has been recently exploited by the Map equation formalism (M. Rosvall
and C.T. Bergstrom, PNAS, 105(4), pp.1118--1123, 2008) through an
information-theoretic description of the process of coding inter- and
intra-community transitions of a random walker in the network at stationarity.
However, a thorough study of the relationship between the full Markov dynamics
and the coding mechanism is still lacking. We show here that the original Map
coding scheme, which is both block-averaged and one-step, neglects the internal
structure of the communities and introduces an upper scale, the `field-of-view'
limit, in the communities it can detect. As a consequence, Map is well tuned to
detect clique-like communities but can lead to undesirable overpartitioning
when communities are far from clique-like. We show that a signature of this
behavior is a large compression gap: the Map description length is far from its
ideal limit. To address this issue, we propose a simple dynamic approach that
introduces time explicitly into the Map coding through the analysis of the
weighted adjacency matrix of the time-dependent multistep transition matrix of
the Markov process. The resulting Markov time sweeping induces a dynamical
zooming across scales that can reveal (potentially multiscale) community
structure above the field-of-view limit, with the relevant partitions indicated
by a small compression gap.Comment: 10 pages, 6 figure
Prerequisites for Successful Fiscal Reform: Some Preliminary Results
Journal of Public Budgeting, Accounting and Financial Management, Winter 1998.Refereed Journal ArticleThis paper examines whether any relationship exists between
success or failure of policy reform on the one hand, and various
political/economic conditions in place at the time of reform on the other.
Nineteen countries were scored using three financial variables to measure the
degree of success or failure of the reform. The independent variables were
country scores for ten different economic and political conditions. The
independent variables were used to try and predict a priori which of the nineteen
countries would succeed and which would fail. Eighteen of the nineteen
countries were correctly placed into their respective success group. However
only three of the ten conditions appeared important in predicting success: a
visionary leader, a crisis, and a comprehensive program. Other writers have
suggested different sets of predicting variables
Drug Hypersensitivity Syndrome toCarbamazepine and Human Herpes Virus 6 Infection: Case Reportand Literature Review
Abstract.: We describe a patient with a drug-induced hypersensitivity syndrome to carbamazepine and a concomitant active infection with human herpes virus 6 (HHV-6). The potential role of HHV-6 regarding the drug-induced hypersensitivity syndrome is discussed and the main clinical features of this potentially fatal adverse drug reaction are highlighte
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