Laparoscopic Transhiatal Esophagectomy at a Low-Volume Center

Results of this study suggest that surgeon experience is more important than the absolute number of procedures performed each year as regards operative morbidity and mortality

Geometry description markup language for physics simulation and analysis applications

The Geometry Description Markup Language (GDML) is a specialized XML-based language designed as an application-independent persistent format for describing the geometries of detectors associated with physics measurements. It serves to implement ''geometry trees'' which correspond to the hierarchy of volumes a detector geometry can be composed of, and to allow to identify the position of individual solids, as well as to describe the materials they are made of. Being pure XML, GDML can be universally used, and in particular it can be considered as the format for interchanging geometries among different applications. In this paper we will present the current status of the development of GDML. After having discussed the contents of the latest GDML schema, which is the basic definition of the format, we will concentrate on the GDML processors. We will present the latest implementation of the GDML ''writers'' as well as ''readers'' for either Geant4 [2], [3] or ROOT [4], [10]

Comparación entre la respuesta de la actividad muscular lumbar en plataforma vibratoria y en ejercicio clásico de squat isométrico en 30º y 60º. (A comparison of the lumbar muscle activity responsein 30º and 60º isometric squat between whole-body vibration and a classic exercise).

<p align="justify">Whole-body vibration (WBV) has improved as a variety of exercises, so it is necessary know muscles responses to the vibration stimulus.The aim of this work was to study and to compare the change in muscle activation in the lumbar area and lower body between the whole-body vibration exercise and classic strength exercises in isometric squat.23 subjects were exposed to six different loads in one of each exercise mode: vibration or classic strength. Both exercises were performed at 30º and 60º semi-squat position. Muscle activity of the lower body and lumbar area was measured using surface electromyography activity (EMG).The results showed that the response of lumbar area in WBV was lower than in the classic strength exercise at the same value of lower body sEMG. Lumbar sEMG was highest for the classic exercise. Moreover, during 30º squat sEMG was higher than during 60ºsquat.</p>Resumen<p align="justify">El aumento del uso de la plataforma vibratoria como forma de realizar ejercicio conlleva la necesidad de conocer las respuestas musculares al estímulo de la misma.El objetivo de este trabajo ha sido estudiar y comparar la respuesta de la activación muscular de la zona lumbar y del tren inferior en el trabajo de plataforma vibratoria con el trabajo clásico de fuerza en un squat isométrico.23 sujetos fueron sometidos a 6 condiciones de vibración y a 6 de un trabajo clásico de pesas. Se analizó la sEMG del tren inferior y de la zona lumbar en un trabajo isométrico de un squat con flexión de 30º y 60º de rodilla.Los resultados muestran que para un mismo valor de sEMG del tren inferior, los valores de sEMG de la zona lumbar son menores en el ejercicio vibratorio. Existiendo en el trabajo de pesas una mayor exigencia en la zona lumbar. Además, con una flexión de 30º la activación muscular es mayor en todas las condiciones. (p<0,05)</p

In vitro activity of pertuzumab in combination with trastuzumab in uterine serous papillary adenocarcinoma

BACKGROUND: Uterine serous papillary adenocarcinoma (USPC) is a rare but highly aggressive variant of endometrial cancer. Pertuzumab is a new humanised monoclonal antibody (mAb) targeting the epidermal growth factor type II receptor (HER2/neu). We evaluated pertuzumab activity separately or in combination with trastuzumab against primary USPC cell lines expressing different levels of HER2/neu. METHODS: Six USPC cell lines were assessed by immunohistochemistry (IHC), flow cytometry, and real-time PCR for HER2/neu expression. c-erbB2 gene amplification was evaluated using fluorescent in situ hybridisation (FISH). Sensitivity to pertuzumab and trastuzumab-induced antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) was evaluated in 5 h chromium release assays. Pertuzumab cytostatic activity was evaluated using proliferation-based assays. RESULTS: Three USPC cell lines stained heavily for HER2/neu by IHC and showed amplification of the c-erbB2 gene by FISH. The remaining FISH-negative USPCs expressed HER2/neu at 0/1\ufe levels. In cytotoxicity experiments against USPC with a high HER2/neu expression, pertuzumab and trastuzumab were similarly effective in inducing strong ADCC. The addition of complementcontaining plasma and interleukin-2 increased the cytotoxic effect induced by both mAbs. In low HER2/neu USPC expressors, trastuzumab was more potent than pertuzumab in inducing ADCC. Importantly, in this setting, the combination of pertuzumab with trastuzumab significantly increased the ADCC effect induced by trastuzumab alone (P\ubc0.02). Finally, pertuzumab induced a significant inhibition in the proliferation of all USPC cell lines tested, regardless of their HER-2/neu expression. CONCLUSION: Pertuzumab and trastuzumab induce equally strong ADCC and CDC in FISH-positive USPC cell lines. Pertuzumab significantly increases tratuzumab-induced ADCC against USPC with a low HER2/neu expression and may represent a new therapeutic agent in patients harbouring advanced/recurrent and/or refractory USPC

Sobrevivência de mudas clonais de erva-mate submetidas a adubação mineral.

Até o momento, poucos foram os avanços na área de nutrição na cultura da erva-mate, o que justifica que o atual sistema de produção seja baseado no extrativismo. O trabalho objetivou avaliar a sobrevivência de clones de erva-mate submetidos a doses de N, P, K, S e micronutrientes (B, Cu e Zn). Nos cinco experimentos, instalados em setembro de 2013 em Três Barras-SC, avaliaram-se quatro clones (F1, F2, F3 e M1) e doses de: 0, 125, 250, 375 e 500 mg dm-3 de N; 0, 75, 150, 225 e 300 mg dm-3 de P2O5; 0, 40, 80, 120 e 160 mg dm-3 de K2O; 0, 20, 40, e 60 mg dm-3 de S; e um Mix de micronutrientes de 0, 0,5 e 1,0 mg dm-3 de B e Cu, e 0, 1,0 e 2,0 mg dm-3 de Zn. Utilizou-se o delineamento blocos casualizados com quatro repetições em esquema fatorial com parcelas subdivididas. As mudas foram propagadas por miniestaquia de matrizes selecionadas, plantadas a campo com altura média de 12 cm. A sobrevivência foi avaliada mensalmente até aos 120 dias. Após os dados submetidos à análise estatística, verificou-se que a época influenciou negativamente a sobrevivência de mudas de erva-mate. A ausência da significância do fator dose na sobrevivência das mudas, possivelmente esteja relacionada a boa fertilidade do solo local. Conclui-se que a adubação não atua na sobrevivência de mudas de erva-mate. Mudas de erva-mate dos clones F1 e F2 são as mais indicadas para plantio na região de Três Barras

hI-con1, a factor VII-IgGFc chimeric protein targeting tissue factor for immunotherapy of uterine serous papillary carcinoma

BACKGROUND: Uterine serous papillary adenocarcinoma (USPC) is a highly aggressive variant of endometrial cancer. Human immunoconjugate molecule (hI-con1) is an antibody-like molecule targeted against tissue factor (TF), composed of two human Factor VII (fVII) as the targeting domain, fused to human immunoglobulin (Ig) G1 Fc as an effector domain. We evaluated hI-con1 potential activity against primary chemotherapy-resistant USPC cell lines expressing different levels of TF. METHODS: A total of 16 formalin-fixed, paraffin-embedded USPC samples were evaluated by immunohistochemistry (IHC) for TF expression. Six primary USPC cell lines, half of which overexpress the epidermal growth factor type II (HER2/neu) receptor at 3\ufe levels, were assessed by flow cytometry and real-time PCR for TF expression. Sensitivity to hI-con1-dependent cell-mediated cytotoxicity (IDCC) was evaluated in 5-hour-chromium release assays. Finally, to investigate the effect of interleukin-2 (IL-2) on IDCC, 5-h 51Cr assays were also conducted in the presence of low doses of IL-2 (i.e., 50\u2013100 IU ml 1). RESULTS: Cytoplasmic and/or membrane TF expression was observed in all 16 (100%) USPC samples tested by IHC, but not in normal endometrium. High expression of TF was found in 50% (three out of six) of the USPC cell lines tested by real-time PCR and flow cytometry when compared with normal endometrial cells (NECs; Po0.001). Uterine serous papillary adenocarcinoma cell lines overexpressing TF, regardless of their high or low HER2/neu expression, were highly sensitive to IDCC (mean killing\ub1s.d., 65.6\ub13.7%, range 57.5\u201377.0%, Po0.001), although negligible cytotoxicity against USPC was seen in the absence of hI-con1 or in the presence of Rituximab control antibody. The addition of low doses of IL-2 further increased the cytotoxic effect induced by hI-con1 against chemotherapy-resistant USPC. CONCLUSION: hI-con1 induces strong cytotoxicity against primary chemotherapy-resistant USPC cell lines overexpressing TF. The hI-con1 may represent a novel therapeutic agent for the treatment of patients harbouring advanced, recurrent and/or metastatic USPC refractory to standard treatment modalities

BRS 408 e BRS 409: cultivares clonais de erva-mate para produção de massa foliar.

Os plantios de erva-mate (Ilex paraguariensis A. St. Hil.) a partir de mudas seminais de plantas matrizes não selecionadas, apresentam desenvolvimento heterogêneo, com reflexos negativos à produtividade, padronização e qualidade do produto final. Além disso, a propagação sexuada apresenta outros problemas, tais como: ocorrência de plantas que produzem poucas sementes ou não as produzem, a produção de mudas com características diferentes da planta matriz, dificuldades para a quebra de dormência e germinação, e longo período de produção das mudas têm constituído desvantagens à produção via sexuada. Esses fatores limitantes podem ser minimizados ou até eliminados com obtenção de mudas propagadas vegetativamente (clonagem).bitstream/item/169702/1/CT-410-1433-final.pd

Induction of tumour-specific CD8+ cytotoxic T lymphocytes by tumour lysate-pulsed autologous dendritic cells in patients with uterine serous papillary cancer

Uterine serous papillary carcinoma is a highly aggressive variant of endometrial cancer histologically similar to high grade ovarian cancer. Unlike ovarian cancer, however, it is a chemoresistant disease from onset, with responses to combined cisplatinum-based chemotherapy in the order of 20% and an extremely poor prognosis. In this study, we demonstrate that tumour lysate-pulsed autologous dendritic cells can elicit a specific CD8+ cytotoxic T lymphocyte response against autologous tumour target cells in three patients with uterine serous papillary cancer. CTL from patients 1 and 2 expressed strong cytolytic activity against autologous tumour cells, did not lyse autologous lymphoblasts or autologous EBV-transformed cell lines, and were variably cytotoxic against the NK-sensitive cell line K-562. Patient 3 CD8+ T cells expressed a modest but reproducible cytotoxicity against autologous tumour cells only at the time of the first priming. Further priming attempts with PBL collected from patient 3 after tumour progression in the lumboaortic lymph nodes were unsuccesful. Cytotoxicity against autologous tumour cells could be significantly inhibited by anti-HLA class I (W6/32) and anti-LFA-1 MAbs. Highly cytotoxic CD8+ T cells from patients 1 and 2 showed a heterogeneous CD56 expression while CD56 was not expressed by non-cytotoxic CD8+ T cells from patient 3. Using two colour flow cytometric analysis of intracellular cytokine expression at the single cell level, a striking dominance of IFN-γ expressors was detectable in CTL populations of patients 1 and 2 while in patient 3 a dominant population of CD8+ T cells expressing IL-4 and IL-10 was consistently detected. Taken together, these data demonstrate that tumour lysate-pulsed DC can be an effective tool in inducing uterine serous papillary cancer-specific CD8+ CTL able to kill autologous tumour cells in vitro. However, high levels of tumour specific tolerance in some patients may impose a significant barrier to therapeutic vaccination. These results may have important implications for the treatment in the adjuvant setting of uterine serous papillary cancer patients with active or adoptive immunotherapy

IGF-II promotes neuroprotection and neuroplasticity recovery in a long-lasting model of oxidative damage induced by glucocorticoids

Insulin-like growth factor-II (IGF-II) is a naturally occurring hormone that exerts neurotrophic and neuroprotective properties in a wide range of neurodegenerative diseases and ageing. Accumulating evidence suggests that the effects of IGF-II in the brain may be explained by its binding to the specific transmembrane receptor, IGFII/M6P receptor (IGF-IIR). However, relatively little is known regarding the role of IGF-II through IGF-IIR in neuroprotection. Here, using adult cortical neuronal cultures, we investigated whether IGF-II exhibits long-term antioxidant effects and neuroprotection at the synaptic level after oxidative damage induced by high and transient levels of corticosterone (CORT). Furthermore, the involvement of the IGF-IIR was also studied to elucidate its role in the neuroprotective actions of IGF-II. We found that neurons treated with IGF-II after CORT incubation showed reduced oxidative stress damage and recovered antioxidant status (normalized total antioxidant status, lipid hydroperoxides and NAD(P) H:quinone oxidoreductase activity). Similar results were obtained when mitochondria function was analysed (cytochrome c oxidase activity, mitochondrial membrane potential and subcellular mitochondrial distribution). Furthermore, neuronal impairment and degeneration were also assessed (synaptophysin and PSD-95 expression, presynaptic function and FluoroJade B® stain). IGF-II was also able to recover the long-lasting neuronal cell damage. Finally, the effects of IGF-II were not blocked by an IGF-IR antagonist, suggesting the involvement of IGF-IIR. Altogether these results suggest that, in or model, IGF-II through IGF-IIR is able to revert the oxidative damage induced by CORT. In accordance with the neuroprotective role of the IGF-II/IGF-IIR reported in our study, pharmacotherapy approaches targeting this pathway may be useful for the treatment of diseases associated with cognitive deficits (i.e., neurodegenerative disorders, depression, etc.)