Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.

Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut

Crescimento de frutos de limoeiro sob uso do molhamento parcial do sistema radicular em condições semi-áridas do Norte de Minas.

Mediante o suprimento adequado de água às plantas cítricas, a irrigação pode proporcionar maior pegamento de flores e frutos garantindo, também, maior produtividade. Estudos recentes têm mostrado que o molhamento parcial do sistema radicular (PRD) é uma importante técnica de irrigação que tende a diminuir o uso agrícola da água, mantendo o vigor dos frutos e o rendimento das culturas quando comparados com os métodos de irrigação normal. O objetivo do trabalho foi avaliar o efeito da técnica do molhamento parcial do sistema radicular no crescimento de frutos do limoeiro Taithi nas condições edafoclimaticas do Norte de Minas em solo de textura arenosa. O experimento seguiu um delineamento em blocos casualizados, com nove tratamentos e quatro repetições. Os tratamentos foram baseados na percentagem de redução (PRL) da lâmina calculada de irrigação, sendo T1 50% redução da lamina de irrigação (LI) e 7 dias de freqüência de alternância (FA), T2 50% LI e 14 FA, T3 50%LI e 21 FA, T4 50% LI e 28 FA, T5 25% Li e 7 FA, T6 25% Li e 14 FA, T7 25% LI e 21 FA, T8 25% Li e 28 FA e T9 com irrigação total (controle). Na média geral de todos os tratamentos houve diferença entre as laminas, com os maiores valores encontrado referente à aplicação da redução de 25%. O tratamento 5 apresentou valores próximo ao testemunha, sendo portanto um indicativo claro de que o uso do déficit controlado com redução de 25% e alternância de 7 dias é uma boa alternativa.pdf 234

The step of incorporation of Bacillus coagulans GBI-30 6086 into “requeijão cremoso” processed cheese does not affect metabolic homeostasis of rats

Dairy product consumption is a common habit in Brazil. These products present a good matrix for probiotic incorporation. Thus, in this study the feasibility of producing a probiotic "requeijao cremoso" incorporated with Bacillus coagulans GBI-30 6086 in three different steps and its metabolic effect in an animal model for 2 weeks has been evaluated. Wistar adult health rats were randomized into one to five groups (n = 8 for each group): Control (C); "requeijao cremoso" without probiotic (RC); probiotic inoculated in the milk before pasteurization at 65 degrees C/30 min (RPP); "requeijao cremoso" inoculated before the fusion step and consequently exposed to 90 degrees C/5 min (RPF); and "requeijao cremoso" inoculated after fusion step, i.e., once the product temperature reached 50 degrees C (RPAF). At the end of treatment, analysis of molecular markers of proteins of stress and antioxidant system, HSP 25, 60, 70 and 90, SOD and catalase were performed in the animals' muscles by Western Blot technique. The HSP25, HSP90 and catalase levels of C, RPP, RPF, and RPAF were similar, indicating that the homeostasis remained unchanged. The incorporation of B. coagulans GBI-30 6086 in the "requeijao cremoso" was shown to be stable and the microorganism remained viable in all steps tested. The incorporation of the probiotic strain in the fusion stage facilitated the technological process, since it allowed a better homogenization of the product and did not affect the maintenance of the metabolic homeostasis of rats10CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informação302763/2014-7; 305804/2017-013/21544-9; 18/24540-8; 2019/21188-

BRS Bonança: upland rice cultivar.

BRS Bonanca is an upland rice cultivar developed by Embrapa Rice and Beans and recommended for sowing in the states of Goias, Maranha, Mato Grosso, and Piaui. BRS Bonanca average grain yields were higher than the checks: flowered 80 days after germination; presented good cooking quality and resistance to the main rice diseases