Formulation development and delivery of biopesticides

Non-Peer ReviewedBiopesticide formulation development is integral for end product development and risk reduction associated with commercialization and acceptance by the end user. Development of robust formulations for biopesticides is a key step towards advancing this technology into integrated pest management systems. A granular formulation protocol using extrusion-spheronization-fluidized bed drying for biopesticidal bacteria and fungal hypha and spores is described. Establishing low granule water activity (aw, 0.2-0.3) is a key factor in extending the shelf-life of the product. Starch type and amount provided controlled release attributes to the biopesticide granules. Microencapsulation of bioherbicide, Colletotrichum truncatum 00-003B1 (Ct), conidia and bioinsecticide nucleopolyhedrovirus (NPV), by complex coacervation is described for foliar application of biocontrol agents

Gravimagnetic effect of the barycentric motion of the Sun and determination of the post-Newtonian parameter gamma in the Cassini experiment

The most precise test of the post-Newtonian gamma parameter in the solar system has been achieved in measurement of the frequency shift of radio waves to and from the Cassini spacecraft as they passed near the Sun. The test relies upon the JPL model of radiowave propagation that includes, but does not explicitly parametrize, the impact of the non-stationary component of the gravitational field of the Sun, generated by its barycentric orbital motion, on the Shapiro delay. This non-stationary gravitational field of the Sun is associated with the Lorentz transformation of the metric tensor and the affine connection from the heliocentric to the barycentric frame of the solar system and can be treated as gravimagnetic field. The gravimagnetic field perturbs the propagation of a radio wave and contributes to its frequency shift at the level up to 4 10^{-13} that may affect the precise measurement of the parameter gamma in the Cassini experiment to about one part in 10,000. Our analysis suggests that the translational gravimagnetic field of the Sun can be extracted from the Cassini data, and its effect is separable from the space curvature characterized by the parameter gamma.Comment: 12 pages, 1 figure, accepted to Physical Letters

Supersymmetric Euler-Heisenberg effective action: Two-loop results

The two-loop Euler-Heisenberg-type effective action for N = 1 supersymmetric QED is computed within the background field approach. The background vector multiplet is chosen to obey the constraints D_\a W_\b = D_{(\a} W_{\b)} = const, but is otherwise completely arbitrary. Technically, this calculation proves to be much more laborious as compared with that carried out in hep-th/0308136 for N = 2 supersymmetric QED, due to a lesser amount of supersymmetry. Similarly to Ritus' analysis for spinor and scalar QED, the two-loop renormalisation is carried out using proper-time cut-off regularisation. A closed-form expression is obtained for the holomorphic sector of the two-loop effective action, which is singled out by imposing a relaxed super self-duality condition.Comment: 27 pages, 2 eps figures, LaTeX; V2: typos corrected, comments and reference adde

Slepton Flavor Nonuniversality, the Muon EDM and its Proposed sensitive Search at Brookhaven

We analyze the electric dipole moment of the electron ($d_e$), of the neutron ($d_n$) and of the muon ($d_{\mu}$) using the cancellation mechanism in the presence of nonuniversalities of the soft breaking parameters. It is shown that the nonuniversalities in the slepton sector produce a strong violation of the scaling relation $d_{\mu}/d_e\simeq m_{\mu}/m_e$ in the cancellation region. An analysis of $d_e, d_n$ and $d_{\mu}$ under the constraints of the current experimental limits on $d_e$ and $d_n$ and under the constraints of the recent Brookhaven result on $g_{\mu}-2$ shows that in the non-scaling region $d_{\mu}$ can be as large as ($10^{-24}-10^{-23}$)ecm and thus within reach of the recently proposed Brookhaven experiment for a sensitive search for $d_{\mu}$ at the level of $10^{-24}$ ecm.Comment: 24 pages, Latex, including 5 figures with additional reference

Applications of Strand-Specific in situ Hybridization

This is the final report of a three-year, Laboratory-Directed Research and Development (LDRD) project at the Los Alamos National Laboratory (LANL). Fluorescence in situ hybridization (FISH) is used to determine the location of specific DNA sequences on chromosomes. It is an effective tool in genomic mapping and is finding increasing use in medical diagnosis. A ''strand-specific'' version of FISH has been developed in the Life Sciences Division of LANL. The new procedure, named CO-FISH, reveals not only location but also the 5'-to-3'direction of a target sequence, such as the sense strand of a gene. This project was designed to investigate applications of the new technique. Strand-specific FISH was found to be useful and informative for genomic mapping of repetitive DNA sequences. The method provide a valuable new tool for investigating the mechanisms of aneuploidy inducing agents and the cytogenetic phenomena called lateral asymmetry. Finally, using strand-specific FISH, the authors were able to detect certain types of chromosome aberrations (isochromosomes, inversions and Robertsonian translocations) that can be difficult to observe with standard techniques

NATO Survey of Mental Health Training in Army Recruits

To-date, there has been no international review of mental health resilience training during Basic Training nor an assessment of what service members perceive as useful from their perspective. In response to this knowledge gap, the North Atlantic Treaty Organization (NATO) Human Factors & Medicine Research & Technology Task Group “Mental Health Training” initiated a survey and interview with seven to twenty recruits from nine nations to inform the development of such training (N = 121). All nations provided data from soldiers joining the military as volunteers, whereas two nations also provided data from conscripts. Results from the volunteer data showed relatively consistent ranking in terms of perceived demands, coping strategies, and preferences for resilience skill training across the nations. Analysis of data from conscripts identified a select number of differences compared to volunteers. Subjects also provided examples of coping with stress during Basic Training that can be used in future training; themes are presented here. Results are designed to show the kinds of demands facing new recruits and coping methods used to overcome these demands to develop relevant resilience training for NATO nations

An Assessment of the Use of Chimpanzees in Hepatitis C Research Past, Present and Future: 1. Validity of the Chimpanzee Model

The USA is the only significant user of chimpanzees in biomedical research in the world, since many countries have banned or limited the practice due to substantial ethical, economic and scientific concerns. Advocates of chimpanzee use cite hepatitis C research as a major reason for its necessity and continuation, in spite of supporting evidence that is scant and often anecdotal. This paper examines the scientific and ethical issues surrounding chimpanzee hepatitis C research, and concludes that claims of the necessity of chimpanzees in historical and future hepatitis C research are exaggerated and unjustifiable, respectively. The chimpanzee model has several major scientific, ethical, economic and practical caveats. It has made a relatively negligible contribution to knowledge of, and tangible progress against, the hepatitis C virus compared to non-chimpanzee research, and must be considered scientifically redundant, given the array of alternative methods of inquiry now available. The continuation of chimpanzee use in hepatitis C research adversely affects scientific progress, as well as chimpanzees and humans in need of treatment. Unfounded claims of its necessity should not discourage changes in public policy regarding the use of chimpanzees in US laboratories