The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria

Quantum information processing using Josephson junctions coupled through cavities

Josephson junctions have been shown to be a promising solid-state system for implementation of quantum computation. The significant two-qubit gates are generally realized by the capacitive coupling between the nearest neighbour qubits. We propose an effective Hamiltonian to describe charge qubits coupled through the cavity. We find that nontrivial two-qubit gates may be achieved by this coupling. The ability to interconvert localized charge qubits and flying qubits in the proposed scheme implies that quantum network can be constructed using this large scalable solid-state system.Comment: 5 pages, to appear in Phys Rev A; typos corrected, solutions in last eqs. correcte

Assessing the damming effects on runoff using a multiple linear regression model: A case study of the Manwan Dam on the Lancang River

AbstractThe Lancang River in Yunnan Province, with a length of 1170km and a 1780-m drop from northwest to southeast, is the most controversial river in southwest China because 14 cascade hydropower stations have been planned on the main waterway. The Manwan Dam, the first of the 14 dams, began operating in 1993, and the associated downstream runoff may have been affected by its construction. To assess this impact, we first investigated the relationships between monthly runoff observed from the Gajiu station and meteorological data obtained from four meteorological gauging stations with a time-lag of 0-3 months over the pre-dam period (1957-2000). Second, we established and validated a multiple linear regression equation employing monthly meteorological and hydrological data during the pre-dam period. Finally, we simulated the monthly runoff after dam construction (1993-2000) using the established equations and assessed the impact of dam construction on runoff by comparing the observed actual monthly runoff with the simulated monthly runoff. Our results suggested a very high hydro-meteorological correlation for the pre-dam period, which opened up the possibility of runoff forecasting. Further, the multiple linear regression equation displayed good simulation performance as coefficient of determination (R2) and the Nash-Suttcliffe coefficient (NS) reached 0.84 and 0.82 respectively. By comparing the observed and the predicted monthly runoff, we found that construction of the Manwan Dam caused a visible disturbance on monthly runoff that, with the disturbance value, displayed a multi-peak fluctuation of up-down variation in the annual hydrologic regime circl

Development of a multiplex event-specific PCR assay for detection of genetically modified rice

Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries. Faster and more effective detection of GM contamination can prevent adulterated food, feed and seed from being consumed and grown, minimize the potential environmental, health or economic damage. In this study, a simple, reliable and cost-effective multiplex polymerase chain reaction (PCR) assay for identifying genetic modifications of TT51-1, Kemingdao1 (KMD1) and Kefeng6 (KF6) rice was developed by using the event-specific fragment. The limit of detection (LOD) for each event in the multiplex PCR is approximately 0.1%. Developed multiplex PCR assays can provide a rapid and simultaneous detection of GM rice

Skyrmions in Higher Landau Levels

We calculate the energies of quasiparticles with large numbers of reversed spins (``skyrmions'') for odd integer filling factors 2k+1, k is greater than or equals 1. We find, in contrast with the known result for filling factor equals 1 (k = 0), that these quasiparticles always have higher energy than the fully polarized ones and hence are not the low energy charged excitations, even at small Zeeman energies. It follows that skyrmions are the relevant quasiparticles only at filling factors 1, 1/3 and 1/5.Comment: 10 pages, RevTe

Anisotropic Transport of Quantum Hall Meron-Pair Excitations

Double-layer quantum Hall systems at total filling factor $\nu_T=1$ can exhibit a commensurate-incommensurate phase transition driven by a magnetic field $B_{\parallel}$ oriented parallel to the layers. Within the commensurate phase, the lowest charge excitations are believed to be linearly-confined Meron pairs, which are energetically favored to align with $B_{\parallel}$. In order to investigate this interesting object, we propose a gated double-layer Hall bar experiment in which $B_{\parallel}$ can be rotated with respect to the direction of a constriction. We demonstrate the strong angle-dependent transport due to the anisotropic nature of linearly-confined Meron pairs and discuss how it would be manifested in experiment.Comment: 4 pages, RevTex, 3 postscript figure

Carbon doping of superconducting magnesium diboride

We present details of synthesis optimization and physical properties of nearly single phase carbon doped MgB2 with a nominal stoichiometry of Mg(B{0.8}C{0.2})2 synthesized from magnesium and boron carbide (B4C) as starting materials. The superconducting transition temperature is ~ 22 K (~ 17 K lower than in pure MgB2). The temperature dependence of the upper critical field is steeper than in pure MgB2 with Hc2(10K) ~ 9 T. Temperature dependent specific heat data taken in different applied magnetic fields suggest that the two-gap nature of superconductivity is still preserved for carbon doped MgB2 even with such a heavily suppressed transition temperature. In addition, the anisotropy ratio of the upper critical field for T/Tc ~ 2/3 is gamma ~ 2. This value is distinct from 1 (isotropic) and also distinct from 6 (the value found for pure MgB2).Comment: 11 pages, 13 figures, submitted to Physica