Remote identification of sheep with flystrike using behavioural observations

Flystrike is a major problem affecting sheep in Australia. Identification of ‘flystruck’ individuals is crucial for treatment; but requires labour-intensive physical examination. As the industry moves toward more low-input systems; there is a need for remote methods to identify flystruck individuals. The aim of this study was to investigate the behaviour of sheep with breech flystrike within a paddock setting. Video footage of sixteen Merino sheep; eight later confirmed with flystrike and eight without; was collected as they moved freely within the paddock with conspecifics. Quantitative behavioural measurements and a qualitative behavioural assessment (QBA) were conducted and compared to their breech conditions (i.e., faecal/urine staining; flystrike severity). Both qualitative and quantitative assessments indicated behavioural differences between flystruck and non-flystruck animals. Flystruck sheep had a behavioural profile characterised by restless behaviour; abnormal postures and reduced grazing time (<i>p</i> < 0.05). Furthermore; flystruck sheep were scored to have a more ‘exhausted/irritated’ demeanour using QBA (<i>p</i> < 0.05). The behavioural responses also corresponded to the flystrike severity scores and condition of the breech area. We conclude that remotely assessed behaviour of flystruck sheep diverges markedly from non-flystruck sheep; and thus could be a low-input method for identifying and treating affected animals

The contribution of qualitative behavioural assessment to appraisal of livestock welfare

Animal welfare is increasingly important for the Australian livestock industries, to maintain social licence to practice as well as ensuring market share overseas. Improvement of animal welfare in the livestock industries requires several important key steps. Paramount among these, objective measures are needed for welfare assessment that will enable comparison and contrast of welfare implications of husbandry procedures or housing options. Such measures need to be versatile (can be applied under a wide range of on- and off-farm situations), relevant (reveal aspects of the animal’s affective or physiological state that is relevant to their welfare), reliable (can be repeated with confidence in the results), relatively economic to apply, and they need to have broad acceptance by all stakeholders. Qualitative Behavioural Assessment (QBA) is an integrated measure that characterises behaviour as a dynamic, expressive body language. QBA is a versatile tool requiring little specialist equipment suiting application to in situ assessments that enables comparative, hypothesis-driven evaluation of various industry-relevant practices. QBA is being increasingly used as part of animal welfare assessments in Europe, and although most other welfare assessment methods record ‘problems’ (e.g. lameness, injury scores, and so on), QBA can capture positive aspects of animal welfare (e.g. positively engaged with their environment, playfulness). In this viewpoint, we review the outcomes of recent QBA studies and discuss the potential application of QBA, in combination with other methods, as a welfare assessment tool for the Australian livestock industries

Review of livestock welfare indicators relevant for the Australian live export industry

Animal welfare is an important issue for the live export industry (LEI), in terms of economic returns, community attitudes and international socio-political relations. Mortality has traditionally been the main welfare measure recorded within the LEI; however, high mortality incidents are usually acted upon after adverse events occur, reducing the scope for proactive welfare enhancement. We reviewed 71 potential animal welfare measures, identifying those measures that would be appropriate for use throughout the LEI for feeder and slaughter livestock species, and categorised these as animal-, environment- and resource-based. We divided the live export supply chain into three sectors: (1) Australian facilities, (2) vessel and (3) destination country facilities. After reviewing the relevant regulations for each sector of the industry, we identified 38 (sector 1), 35 (sector 2) and 26 (sector 3) measures already being collected under current practice. These could be used to form a ‘welfare information dashboard’: a LEI-specific online interface for collecting data that could contribute towards standardised industry reporting. We identified another 20, 25 and 28 measures that are relevant to each LEI sector (sectors 1, 2, 3, respectively), and that could be developed and integrated into a benchmarking system in the future

Varying opinions about animal welfare in the Australian live export industry: A survey

There is significant public interest in the Australian live animal export industry and a need to develop a program that can measure and monitor animal welfare throughout the supply chain. An online survey of stakeholder opinions of this industry and animal welfare measures was carried out in 2015 with 921 respondents: 30% from the public who identified as animal welfare advocates (AWAs); 44% from the public who did not identify as AWAs (general public; GP); 26% live export industry (LEI) workers. AWA and GP respondents expressed greater concern than LEI respondents for animal welfare throughout the supply chain but had less concern for animals at Australian feedlots than in other parts of the supply chain. The majority of AWA and GP respondents believed data collected on animal welfare should be made public and should be collected by independent welfare officers and used to regulate the industry and impose penalties for poor welfare. LEI workers believed that data should be confidential, collected by LEI workers and used by the industry to self-regulate. AWA and GP respondents rated the importance and practicality of a number of welfare indicators greater than LEI workers, while respondents shared an analogous view of the importance and practicality of these indicators. Results can be used to develop welfare assessments that ensure a better understanding between industry members and those not in the industry, while facilitating welfare improvements and promoting greater transparency for the live export industry

Preliminary findings on a novel behavioural approach for the assessment of pain and analgesia in lambs subject to routine husbandry procedures

The identification and assessment of pain in sheep under field conditions are important, but, due to their stoic nature, are fraught with many challenges. In Australia, various husbandry procedures that are documented to cause pain are routinely performed at lamb marking, including ear tagging, castration, mulesing, and tail docking. This study evaluated the validity of a novel methodology to assess pain in lambs: qualitative behavioural assessment (QBA) was used to compare the behavioural expression of control lambs (CONTROL) with that of lambs subject to these procedures that received either a saline placebo 15 min before procedures (PLACEBO), or were administered meloxicam 15 min before procedures in addition to the standard analgesic Tri-Solfen at the time of procedures, as per the manufacturer’s recommendations (ANALGESIC TREATMENT; AT). In terms of behavioural expression, it was expected that: CONTROL ≠ PLACEBO, AT = CONTROL, and PLACEBO ≠ AT. Video footage of the 6−8-week-old lambs (n = 10 for each treatment) was captured approximately 1.5 h postprocedure and was presented, in a random order, to 19 observers for assessment using the Free-Choice Profiling (FCP) approach to QBA. There was significant consensus (p < 0.001) among the observers in their assessment of the lambs, with two main dimensions of behavioural expression explaining 69.2% of the variation. As expected, observers perceived differences in the demeanour of lambs in the first dimension, scoring all lambs subject to the routine husbandry procedures as significantly more ‘dull’ and ‘uneasy’ compared to the control lambs (p < 0.05). Contrary to expectations, the results also suggested that analgesic treatment did not provide relief at the time of observation. Further investigations to validate the relationship between behavioural expression scores and pain are necessary, but these results suggest that painful husbandry procedures alter the behavioural expression of lambs and these differences can be captured using QBA methodology

Behavioural assessment of the habituation of feral rangeland goats to an intensive farming system

There is increasing interest in methods for the habituation of feral rangeland goats to intensive farming conditions. We tested whether there were production performance and behavioural differences between groups of rangeland goats in an intensive farming system that were either exposed to a high degree of human interaction (HI, n=60) or low degree of human interaction (LI, n=60) over 3 weeks. In the HI group, a stockperson entered the pens twice daily and calmly walked amongst the goats for 20 mins. In the LI group, a stockperson only briefly entered the pens to check water/feed (daily/weekly). At the end of each week the goats were weighed and drafted into 12 subgroups of 10 animals (i.e. 6 sub-groups per treatment). Each sub-group was then tested for agonistic behaviour, avoidance of humans, and flight response. During the flight response test video footage was collected and later used for analysis using Qualitative Behavioural Assessment (QBA). For QBA analysis, the videos of each group, taken each week, were shown in random order to 16 observers who used their own descriptive terms to score the groups' behavioural expression. There was a significant interaction between treatment and time on body mass (F3,174 =5.0; P< 0.01), agonistic behaviour (F3,12 =4.3; P< 0.05) and flight speed (F3,12 =3.9; P< 0.05), with the HI group having significantly higher average body mass (P< 0.05), fewer agonistic events (P< 0.05), and a slower flight speed (P< 0.05) than the LI group after the three weeks. Two main QBA dimensions of behavioural expression were identified by Generalised Procrustes Analysis. QBA dimension 1 scores differed between treatments (P< 0.05); HI goats scored higher on QBA dimension 1 (more 'calm/content') compared to LI goats (more 'agitated/scared'). QBA dimension 1 scores were significantly negatively correlated with the number of agonistic contacts (Rs =-0.62, P< 0.01), and flight speed (Rs =-0.79, P< 0.001), and significantly positively correlated with body mass (Rs =0.68, P <0.001) of the goats over the 3 weeks of the experiment. QBA dimension 2 scores were not significantly different between treatments or over time.Findings from this study support the hypothesis that production performance and behavioural measures can distinguish behavioural changes in rangeland goats that were likely a result of habituation to human interaction in an intensive feedlot

Animal behaviour as a measure of animal welfare

Animal welfare is increasingly important for the Australian livestock industries, to maintain the social licence to practice, as well as ensuring market share overseas

Qualitative behavioural assessment of the motivation for feed in sheep in response to altered body condition score

Qualitative behavioural assessment (QBA) has been used to quantify the expressive behaviour of animals, and operant tests have been used to quantify measures of behavioural need. In this study we compared measures of behavioural expression and behaviour in operant tests. We examined the behavioural expression of pregnant ewes of body condition score (BCS) 2 and 3. The ewes were exposed to a feed motivation test in which they received a food reward. Pregnant ewes (48-70 days gestation) were assessed during a food motivation test after they had been maintained at BCS 3 (n ≤ 7) or given a decreasing plane of nutrition that resulted in slow loss of 1 BCS unit (over 10-12 weeks; n ≤ 7) or a fast loss of 1 BCS unit (over 4-6 weeks; n ≤ 7). The feed motivation test involved ewes having the opportunity to approach a food reward and then being moved a given distance away from the reward by an automatic gate; they could then subsequently return to the feeder. Continuous video footage of each ewe during one cycle of the gate (approaching and returning from the food reward) was shown in random order to 11 observers who used their own descriptive terms (free-choice profiling methodology; FCP) to score the animals using QBA. Data of the assessment were analysed with generalised Procrustes analysis (GPA), a multivariate statistical technique associated with FCP. The research group also quantified the feeding behaviour of sheep in the same clips. These behaviours included how sheep approached the feeder, behaviours exhibited at the feeder, and how sheep returned from the feeder. There was consensus amongst observers in terms of their assessment of behavioural expression of the sheep (P < 0.001). The GPA found three main dimensions of assessed behavioural expression in the sheep, which together explained 44% of the variation observed. GPA dimension 1 differed between the three treatment groups (P < 0.05): ewes maintained at BCS 3 scored low on GPA dimension 1 (i.e. were described as more calm/bored/comfortable) compared with ewes that had a slow declining BCS (described as more interested/anxious/excited). GPA dimension 2 scores were not significantly different between treatment groups. However, quantitative behaviours exhibited by sheep during the clips were correlated with qualitative behavioural assessments made by the observers. Animals that spent more time 'sniffing and looking for more feed' were attributed lower GPA 2 scores (described as more hungry/searching/excited) (P < 0.05), and animals that 'did not walk directly to the food reward (but stopped along the way)' were attributed significantly higher GPA 2 scores (more curious/intimidated/uneasy) (P < 0.01). GPA dimension 3 scores also did not differ between the treatment groups; however, sheep that had a higher number of feeding events during the entire 23-h feed motivation test were attributed lower GPA dimension 3 scores (they were described as more hungry/bold/interested) (P < 0.05), and sheep that consumed a larger amount of the feed reward were attributed higher GPA dimension 3 scores (more curious/concerned/reserved) (P < 0.05). We conclude that QBA is a valuable method of assessing sheep behavioural expression under the conditions tested, in that it provided an integrative characterisation of sheep behavioural expression that was in agreement with quantitative behavioural measures of feeding

Behavioural assessment of sheep is sensitive to level of gastrointestinal parasite infection

Qualitative behavioural assessment (QBA) was applied to investigate the expressive behaviour of sheep with varying intestinal parasite burdens over two experiments. The expressive behaviour of sheep naturally infected with intestinal parasites was assessed from video footage collected in the paddock, and assessments were compared pre- and post-treatment with anthelmintic drench. The first experiment assessed sheep with a range of parasite burdens (n = 28), and the second compared sheep that expressed clinical symptoms of parasitism (Anaemic, n = 5) with those that did not (Non-anaemic, n = 5). Behavioural expression scores were validated against individual clinical evaluations (faecal egg counts – FEC, faecal consistency and anaemia scores), production parameters (body mass and body condition score), and quantitative locomotive measures (walking speed and return order to paddock). Twenty-two observers scored 28 video clips using QBA in experiment 1, and in the second experiment, 35 observers scored 20 video clips that depicted the 10 focal sheep pre- and post-treatment. QBA scores were analysed using Generalised Procrustes Analysis (GPA), and sheep scores on the main GPA dimensions were evaluated in relation to parasite burden using Spearman Rank correlations and repeated-measures ANOVA for experiment 1 and 2, respectively. In both experiments, observers reached significant (P <  0.001) consensus in their assessment of the behavioural expression of sheep. In experiment 1, sheep with higher FEC had lower anaemia scores (indicative of anaemia), poor body condition, lower body mass, walked slower, ranked lower in return order, and were scored by observers as more docile/at ease, and more assertive/motivated on GPA dimensions 1 and 3, respectively (P < 0.05). In experiment 2, observers scored Anaemic sheep as significantly more unsettled/apprehensive compared to Non-anaemic sheep on GPA dimension 1 (P <  0.05). In addition, observers consistently scored Non-anaemic sheep as more bright/observant following treatment on dimension 2 (P <  0.001). On dimension 3, all animals were scored by observers as less depressed/suspicious post-treatment (P <  0.05). An increase in average walking speed across all sheep was also identified post-treatment (P <  0.05). We conclude that under the conditions tested, QBA could provide information on the behavioural expression of sheep related to varying parasite burdens and the impact of treatment. The data presented herein offers proof of concept that assessments of behavioural expression could aid producers in the monitoring and management of parasitised sheep on-farm in terms of identification of individuals for treatment, and efficacy of anthelmintic treatment