An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine

A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe vera-based membranes. The chitosan/aloe vera-based membranes that were developed displayed satisfactory degradation, roughness, wettability and mechanical properties. A higher antibacterial potency was displayed by the blended membranes. Moreover, in vitro assays demonstrated that these blended membranes have good cell compatibility with primary human dermal fibroblasts. The chitosan/aloe vera-based membranes might be promising wound dressing materials.The authors acknowledge financial support from the Portuguese Foundation for Science and Technology (grants SFRH/BPD/45307/2008 and SFRH/BD/64601/2009), the "Fundo Social Europeu", and the "Programa Diferencial de Potencial Humano". This work was partially supported by the FEDER through POCTEP 0330_IBEROMARE_1_P

Adhesive free-standing multilayer films containing sulfated levan for biomedical applications

This work is the first reporting the use of layer-by-layer to produce adhesive free-standing (FS) films fully produced using natural-based macromolecules: chitosan (CHI), alginate (ALG) and sulfated levan (L-S). The deposition conditions of the natural polymers were studied through zeta potential measurements and quartz crystal microbalance with dissipation monitoring analysis. The properties of the FS films were evaluated and compared with the control ones composed of only CHI and ALG in order to assess the influence of levan polysaccharide introduced in the multilayers. Tensile tests, dynamic mechanical analysis and single lap shear strength tests were performed to evaluate the mechanical properties of the prepared FS films. The presence of L-S conferred both higher tensile strength and shear strength to the developed FS membranes. The results showed an adhesion strength 4 times higher than the control (CHI/ALG) FS films demonstrating the adhesive character of the FS films containing L-S. Morphological and topography studies were carried out revealing that the crosslinking reaction granted the L-S based FS film with a higher roughness and surface homogeneity. Preliminary biological assays were performed by cultivating myoblasts cells on the surface of the produced FS films. Both crosslinked and uncrosslinked FS films containing L-S were cytocompatible and myoconductive.publishe

Chitosan/bioactive glass nanoparticle composite membranes for periodontal regeneration

Item does not contain fulltextBarrier membranes are used in periodontal applications with the aim of supporting periodontal regeneration by physically blocking migration of epithelial cells. The present work proposes a combination of chitosan (CHT) with bioactive glass nanoparticles (BG-NPs) in order to produce a novel guided tissue and bone regeneration membrane, fabricated by solvent casting. The CHT/BG-NP nanocomposite membranes are characterized in terms of water uptake, in mechanical tests, under simulated physiological conditions and in in vitro bioactivity tests. The addition of BG-NPs to CHT membranes decreased the mechanical potential of these membranes, but on the other hand the bioactivity improved. The membranes containing the BG-NPs induced the precipitation of bone-like apatite in simulated body fluid (SBF). Biological tests were carried out using human periodontal ligament cells and human bone marrow stromal cells. CHT/BG-NP composite membranes promoted cell metabolic activity and mineralization. The results indicate that the CHT/BG-NP composite membrane could potentially be used as a temporary guided tissue regeneration membrane in periodontal regeneration, with the possibility to induce bone regeneration

Asymmetric PDLLA membranes containing Bioglass(R) for guided tissue regeneration: characterization and in vitro biological behavior

Item does not contain fulltextOBJECTIVE: In the treatment of periodontal defects, composite membranes might be applied to protect the injured area and simultaneously stimulate tissue regeneration. This work describes the development and characterization of poly(d,l-lactic acid)/Bioglass(R) (PDLLA/BG) composite membranes with asymmetric bioactivity. We hypothesized that the presence of BG microparticles could enhance structural and osteoconductivity performance of pure PDLLA membranes. METHODS: The membranes were prepared by an adjusted solvent casting method that promoted a non-uniform distribution of the inorganic component along the membrane thickness. In vitro bioactive behavior (precipitation of an apatite layer upon immersion in simulated body fluid, SBF), SEM observation, FTIR, swelling, weight loss and mechanical properties of the developed biomaterials were evaluated. Cell behavior on the membranes was assessed using both human bone marrow stromal cells and human periodontal ligament cells. RESULTS: Just the BG rich face of the composite membranes induced the precipitation of bone-like apatite in SBF, indicating that this biomaterial exhibit asymmetric osteoconductive properties. SEM images, DNA content and metabolic activity quantification revealed an improved cell adhesion and proliferation on the composite membranes. Composite membranes also stimulated cell differentiation, mineralization, and production of extracellular matrix and calcium nodules, suggesting the positive effect of adding the bioactive microparticles in the PDLLA matrix. SIGNIFICANCE: The results indicate that the proposed asymmetric PDLLA/BG membranes could have potential to be used in guided tissue regeneration therapies or in orthopedic applications, with improved outcomes

Biomechanical and cellular segmental characterization of human meniscos: building the basis for Tissue Engineering therapies

"Published online: July 16, 2014"Objective To overcome current limitations of Tissue Engineering (TE) strategies, deeper comprehension on meniscus biology is required. This study aims to combine biomechanical segmental analysis of fresh human meniscus tissues and its correlation with architectural and cellular characterization. Method Morphologically intact menisci, from 44 live donors were studied after division into three radial segments. Dynamic mechanical analysis (DMA) was performed at physiological-like conditions. Micro-computed tomography (CT) analysis of freeze-dried samples assessed micro-structure. Flow cytometry, histology and histomorphometry were used for cellular study and quantification. Results Anterior segments present significantly higher damping properties. Mid body fresh medial meniscus presents higher values ofE′ compared to lateral. Cyclic loads influence the viscoelastic behavior of menisci. By increasing the frequency leads to an increase in stiffness. Conversely, with increasing frequencies, the capacity to dissipate energy and damping properties initially decrease and then rise again. Age and gender directly correlate with higherE’ and tanδ. Micro-CT analysis revealed that mean porosity was 55.5 (21.2–89.8)% and 64.7 (47.7–81.8)% for freeze-dried lateral and medial meniscus, respectively. Predominant cells are positive for CD44, CD73, CD90 and CD105, and lack CD31, CD34 and CD45 (present in smaller populations). Histomorphometry revealed that cellularity decreases from vascular zone 1 to zone 3. Anterior segments of lateral and medial meniscus have inferior cellularity as compared to mid body and posterior ones. Conclusion Menisci are not uniform structures. Anterior segments have lower cellularity and higher damping. Cyclic loads influence viscoelastic characteristics. Future TE therapies should consider segmental architecture, cellularity and biomechanics of fresh tissue.FCT -Fuel Cell Technologies Program(MRTN-CT-2011-289897