Environmental Hazard Identification Technique Developing of Territorial Administrations Strategy as Exemplified in Siberian Federal District

This work, on the example of the Siberian Federal District of the Russian Federation, presents a method for determining mass airbursts of combustion gaseous eco-toxicants, as a technique for assessing the contributory environmental load on the atmosphere. Potential environmental hazards are analyzed by mass of contaminated airbursts. A comparison of specific gross toxicants' emissions in territorial entities of the Russian Federation is made

Public health and public health services: lectures-presentations, computer tests and practical skills

ОБЩЕСТВЕННОЕ ЗДОРОВЬЕОРГАНИЗАЦИЯ ЗДРАВООХРАНЕНИЯУЧЕБНЫЕ ПОСОБИЯРассматриваются современные вопросы общественного здоровья и здравоохранения как науки и предмета преподавания

STRA13 Interacts with STAT3 and Modulates Transcription of STAT3-dependent Targets

STRA13 is a pVHL-dependent bHLH transcription factor up-regulated on the mRNA level in multiple cancer cell lines and implicated recently in the regulation of immune cell homeostasis and autoimmunity. In searching for STRA13-interacting proteins with oncogenic potential by the yeast two-hybrid screening, we identified STAT3β as a STRA13-binding partner. We showed that STRA13 binds predominantly to phosphorylated (active) STAT3 α and β isoforms via its HLH and C-terminal regions. We also found that STRA13 was able to activate transcription from STAT-dependent cis-elements. Expression of endogenous STRA13 was shown to be cytokine-inducible, consistent with STRA13 involvement in STAT-dependent transcription regulation. We demonstrated that the STAT3-regulated promoter of the pro-apoptotic Fas gene was activated upon STRA13 over-expression and that co-expression of STRA13 with STAT3β or STAT3α modulated the transcriptional outcome. Forced expression of STRA13 induced apoptosis, in agreement with the STRA13 activation effect on the Fas promoter. Simultaneous expression of STRA13 and STAT3β resulted in alleviation of the STRA13 pro-apoptotic effect. Thus, for the first time, we identify STRA13 as a STAT3 partner and provide a consistent line of evidence for STRA13 involvement into regulation of apoptosis via the STAT pathways

OPTIMIZATION OF SYNTHESIS AND CHARACTERIZATION OF ZNBTC-BASED MOFS

This work was supported by the Russian Science Foundation (No. 22-73-10069)

Angular Forces Around Transition Metals in Biomolecules

Quantum-mechanical analysis based on an exact sum rule is used to extract an semiclassical angle-dependent energy function for transition metal ions in biomolecules. The angular dependence is simple but different from existing classical potentials. Comparison of predicted energies with a computer-generated database shows that the semiclassical energy function is remarkably accurate, and that its angular dependence is optimal.Comment: Tex file plus 4 postscript figure

Imágenes como herramientas del habla espontánea

The article presents a figurative component in spontaneous speech. It is proved that an image enriches speech, and provides its individualization. Imagery testifies to the creative potential of an individual. It represents a deviation from accepted communicative cliches. Experimental word formation is considered as a source of imagery. A significant role is played by the phenomenon of abbreviation semantics in the process of nomination and renomination.El artículo presenta un componente figurativo en el habla espontánea. Está comprobado que una imagen enriquece el habla y proporciona su individualización. Las imágenes dan testimonio del potencial creativo de un individuo. Representa una desviación de los clichés comunicativos aceptados. La formación experimental de palabras se considera una fuente de imágenes. El fenómeno de la semántica de abreviatura desempeña un papel importante en el proceso de nominación y renominación

Diagnostics of soils located in the gradient of urban-technogenic impact

The rapidly progressing urbanization results in decline of environmentally valuable forest territory. Intact ecosystems bordering urban communities suffer from heavy technogenic pressure and degrade irreversibly over time. This situation necessitates studies that analyze the ecological condition of major components of forest ecosystems exposed to various levels of such pressure. The survey was carried out in the city of Petrozavodsk in sites with degraded, slightly disturbed, and undisturbed forest ecosystems. For each site, the soil condition was assessed, the tree stand and the living ground cover were described. As a result, it was found that soils in the slightly disturbed forest ecosystem generally corresponded to the natural background (Ferric Illuvial Podzol). Changes in their morphological and chemical properties compared to soils in the undisturbed reference plot were identified. The thickness and ash content of the upper organic horizon in the slightly disturbed site changed. The forest ecosystem was in the transitional stage (from I–II to III) of recreational digression and required infrastructural improvements. The forest ecosystem in the built-up site was totally destroyed. Irreversible transformation of its natural environment resulted in the loss of woody and ground vegetation. The native zonal soils were ruined, and their diagnostic horizons cannot be identified. There was observed a large amount of rocks and anthropogenic inclusions throughout the profile. According to the Russian soil classification, these formations belong to the subgroup of organo-mineral strata of technogenic surface formations. The collected data can be used as the basis for ecological monitoring of urban soils. Based on the findings, recommendations were given on how to conserve and improve the sanitary and esthetic functions of forest areas adjoining urban built-up land

Zymography: developing of the enzyme soil activity visualization method

The enzymes produced by the soil biota are a key link in the regulation of biochemical processes. The soil enzyme activity can be visualized with zymography, a method based on using fluorescent substrates and obtaining two-dimensional images (zymograms). A variant of a zymographic measuring system has been proposed. Characteristics of lighting, photographic equipment and shooting modes, reagents preparation and calibration are presented. Preparing and analyzing soil samples of different texture (sand and clay loam) and processing the study results have been described. The ways of introducing the substrate are considered in this study, namely pipetting, short-time dipping, and saturation. An analysis of the kinetics of incubation of samples was carried out. The possibilities and disadvantages of the method were also considered and options for solving possible methodological problems during the analysis were proposed. The zymography is a promising method that allows comparing data with the results of other methods. The use of neural network technologies makes it possible to obtain the volumetric distribution of soil enzymes with high reliability. The soil zymography requires qualitative preparatory work and extreme accuracy during the analysis. It is necessary to ensure maximum contact between the substrate and the soil, as this is one of the key factors determining the quality of the results. The most optimal way to introduce the substrate is to saturate the membranes with substrate solution for 60 minutes. At this stage of the development of the method, it is not possible to establish a universal sample incubation time, since this depends on characteristics of both the studied soils and the experiment conditions. Also, it is necessary to document the conditions in detail for discussion the study results

The influence of aging on shape memory effect in Ti‑50.7at.%Ni and Ti‑51.7at.%Ni single crystals

The shape memory effect (SME) during stressassisted thermal cycles under compressive load in [001]-oriented Ti-50.7at.%Ni and Ti-51.7at.%Ni single crystals aged at 823 K for 1 h has been studied. Ti3Ni4 particles with a diameter of 300–400 nm were precipitated with volume fractions of 11 and 22% and interparticle distances of 300–500 nm and 50–150 nm, respectively. In quenched single crystals, the SME parameters were determined by the transformation type (thermal-induced martensitic transformation (MT) or strain glass transition). In contrast, the SME parameters of aged single crystals were determined by the volume fraction of particles and interparticle distances. Differing volume fractions of particles and interparticle distances led to different temperatures ( M0s ) for the formation of B19′-martensite, different strain (εrev), different dependences of the interval of forward MT ( Δσ1) and thermal hysteresis ( ΔT1= Aσf−Mσs and ΔT2= Aσs−Mσf) on applied stresses, and changes in the morphology of martensite crystals. Practically, these differences do not affect the stresses (σmin and σmax) required to achieve the minimum strain and maximum reversible strain (εrev) and strain growth coefficient (dεrev/dσ). The influence of aging on the dependence of the SME parameters on the chemical composition was analysed in comparison with quenched crystals