A linear model for leaf area measurement to screen potential leaf material for herbal drug in Adhatoda vasica L.

Leaf area is an important parameter in physiology and agronomy studies. Linear models for leaf area measurement are developed for plant species as a nondestructive method. The plant Adhatoda vasica L. (a medicinal plant) was selected and the leaves of this plant were used for development of linear model for leaf area using Leaf Area Meter (LAM) software. Planimetric parameters (length, length2, width and width2) and gravimetric (dry weight and water content) parameters are considered for the development of linear model for this plant species. Single factor ANOVA and linear correlations were worked out using these parameters and leaf area. The plant was showed significant relationship with the parameters studied. The best correlation as represented by regression coefficient (R2) was used and improved R2 is worked out. It is observed that with increase in leaf area, water content is also increased and showed best correlation with the leaf area. Thus water content can be taken as a parameter for developing linear model for leaf area is concluded

Distal subgaleal-peritoneal shunt migration into the abdominal wall with subsequent formation of a pre-peritoneal pseudocyst: a rare complication.

Distal ventriculo-peritoneal shunt migration and extra-peritoneal CSF pseudocyst formation are unusual complications of shunt placement. We present a 65-year-old-female who received a subgaleal-peritoneal shunt to decompress a post-surgical subgaleal fluid collection. Eight weeks later, shunt series showed tight coiling of the distal catheter, and operative exploration found the distal shunt tip to have migrated superficial to the rectus sheath, where it had become encapsulated in a pre-peritoneal CSF pseudocyst. Migration of the distal catheter into the abdominal wall was likely due to local inflammation of the inner surface of the abdomen, with pressure from intestinal peristaltic movements and intra-abdominal pressure, and continued inflammation at the distal catheter tip may have caused formation of a pre-peritoneal CSF pseudocystic dilatation. To date, this is the first reported case of distal shunt migration into the abdominal wall with subsequent formation of an extra-peritoneal pseudocyst and represents a rare event in the surgical management of peritoneal shunts

Analysis for Molecular Distinction in the Chloroplast DNA Sequences of Gymnospora montana (Celastraceae) and Belanites aegyptiaca (Balanitaceae) from Semi-arid Area

Gymnospora montana (Celastraceae) and Belanites aegyptiaca  (Balanitaceae) showed marked similarity in  their cpDNA sequences. Therefore, its detail analysis of cpDNA sequences is performed for codon use bias and its index, relative synonymous codon use value (RSCU), effective number of codons (ENC), GC content of the gene and frequencies of the nucleotides G+C at various positions in synonymous codon were calculated and compared it with Tribulus terresties. Length of the gene and ENC showed close relationship which suggest that longer genes has less codon bias. The codons for leucine, isoleucine and serine were most abundant in the studied plant species. The correlation analysis suggested that codon usage patterns in both cp genomes appear due to the different forces; natural selection, mutation pressure, GC content of gene and protein length.   Their role in gene evolution process is discussed

A Comparative Genomic Analysis of Georgenia sps. for Mining of LysR Transcriptional Regulator Sequences

Georgenia is a genus belonging to the actinomycetes group. The genera comprise only 33 poorly characterized species with reference genomes of 10 distinct species. However, none of the species is well characterized for their genome characteristics. Our laboratory isolate from tomato plant leaf was identified and sequenced for identification and found to be Georgenia sp. Later genomic analysis revealed many functional genes having characteristic functions to be analysed. The source of isolation raised the possibility of having functional genes to enhance senescence or having plant pathogenic activity by Georgenia sp. SUBG003. To explore in silico presence of these genes or gene pools genomic islands were identified and analysed for our isolate and other 10 reference genomes of the Georgenia genus. Genomic islands were further explored for transcription regulators and finally, LysR transcriptional regulator sequences were extracted and a phylogeny among sequences was built from multiple sequence alignment

Analysis and Implementation of Malicious Node in AODV Routing Protocol

The Mobile Ad-hoc Network (MANET) is constructed based on wireless medium and it is of self organizing behaviour. MANET is easy to establish and having dynamic topology. The mobile Ad-hoc networks are vulnerable to various networks attacks because MANET operational environment is open and dynamic or live. MANET uses the Routing protocols for data transfer. Two different types of Routing protocols are available: Table Driven and On Demand Routing Protocols. Malicious node is the one type of mobile node but its work is completely different compared to normal Mobile nodes. Malicious nodes have capability to change or remove Routing Information. It also sends or advertises the fake Route Request to attract user’s data. Malicious node disturbs the Network to carry correct flow of operation. It is responsible for attacks on the existing normal mobile nodes and creates receiver collision, limited transmission power, false misbehaviour etc. Malicious or selfish node carries attacks on the networks so it directly effects to the routing Performance. The objective of this work is to check Network performance in malicious environment and provide prevention for the attack. Throughput and Delay are analysed for Denial of Service (DoS) attack and prevention scenarios. Keywords: MANET, AODV, Selfish Node, DoS Attack, Routing Protocol

Standardization of leaf age for radical scavenging activity in Terminalia arjuna

Terminalia arjuna is one of the many such plant that used in indigenous system of medicine for curing different diseases. For effective formulation of herbal drug selection of proper age of the plant is imperative. In the present study, the T. arjuna plant (leaves) was collected from the Botanical garden of Saurashtra University. The leaves were grouped in to various size and growth analysis was performed. Each extract homogenized in methanol and was used for chlorophyll estimation and antioxidant activities. Radical scavenging activity of the methanol extract of different stages leaves were determined using a stable free radical 2, 2-diphenyl-1-picryl hydrazyl (DPPH), using Micro plate assay. Further the phenolic compounds were separated by Reverse phase high pressure liquid chromatography analysis (RP-HPLC). At the leaf stage when maximum antioxidant activity was observed, phenol like Gallic acid showed distinct peak. The role of phenolic compounds in antioxidant activities is discussed

Molecular and Computational Analysis of Chlorophyll Pigment-binding Protein cp47 from Selected Species of Semi Arid Region of Western India

Photosynthesis means “synthesis with the help of light”, involves the composite functioning of various protein complexes. CP47 is a pigment-binding protein of PSII of a molecular mass of about 56 kDa. CP47, encoded by the chloroplastic psbB gene, is an integral part of the oxygen-evolving complex of PS -II centres. In the present study, analysis of a psbB gene was performed from various tree, shrub, vine and herb species of Saurashtra region. The genomic DNA was isolated from the 46 samples and psbB gene was amplified using specific primers (60R-61F) in PCR. The amplified gene was sequenced from all plant samples and submitted to NCBI database. The length of the amplified sequence was ~300 bp, was translated to the protein sequence. The obtained sequences were analyzed with the help of CPH and Pyre2 tools. The Pyre2 tools showed 40 reliable structure prediction out of 46. ProtParam was used for carrying out the protein physico-chemical analysis of all the proteins showing variations in the protein properties. The number of residues in favored region, as observed in the Ramachandran plot analysis, indicates reliability of the protein structure prediction. The obtained results for the sequence and structure analyses may help to understand the functional application of these proteins