Signature of short distance physics on inflation power spectrum and CMB anisotropy

The inflaton field responsible for inflation may not be a canonical fundamental scalar. It is possible that the inflaton is a composite of fermions or it may have a decay width. In these cases the standard procedure for calculating the power spectrum is not applicable and a new formalism needs to be developed to determine the effect of short range interactions of the inflaton on the power spectrum and the CMB anisotropy. We develop a general formalism for computing the power spectrum of curvature perturbations for such non-canonical cases by using the flat space K\"all\'en-Lehmann spectral function in curved quasi-de Sitter space assuming implicitly that the Bunch-Davis boundary conditions enforces the inflaton mode functions to be plane wave in the short wavelength limit and a complete set of mode functions exists in quasi-de Sitter space. It is observed that the inflaton with a decay width suppresses the power at large scale while a composite inflaton's power spectrum oscillates at large scales. These observations may be vindicated in the WMAP data and confirmed by future observations with PLANCK.Comment: 17 pages, 4 figures, Extended journal version, Accepted for publication in JCA

Cruciform extrusion propensity of human translocation-mediating palindromic AT-rich repeats

There is an emerging consensus that secondary structures of DNA have the potential for genomic instability. Palindromic AT-rich repeats (PATRRs) are a characteristic sequence identified at each breakpoint of the recurrent constitutional t(11;22) and t(17;22) translocations in humans, named PATRR22 (∼600 bp), PATRR11 (∼450 bp) and PATRR17 (∼190 bp). The secondary structure-forming propensity in vitro and the instability in vivo have been experimentally evaluated for various PATRRs that differ regarding their size and symmetry. At physiological ionic strength, a cruciform structure is most frequently observed for the symmetric PATRR22, less often for the symmetric PATRR11, but not for the other PATRRs. In wild-type E. coli, only these two PATRRs undergo extensive instability, consistent with the relatively high incidence of the t(11;22) in humans. The resultant deletions are putatively mediated by central cleavage by the structure-specific endonuclease SbcCD, indicating the possibility of a cruciform conformation in vivo. Insertion of a short spacer at the centre of the PATRR22 greatly reduces both its cruciform extrusion in vitro and instability in vivo. Taken together, cruciform extrusion propensity depends on the length and central symmetry of the PATRR, and is likely to determine the instability that leads to recurrent translocations in humans

A Case of Bifid Mandibular Condyle

Bifid mandibular condyle is a rare anatomic anomaly that can result from congenital malformation, trauma, infection or tumor. We report a case of bifid mandibular condyle found after head injury. A bifid mandibular condyle was seen on the computed tomographic scan of a 41-year-old man after a car accident. The patient had asymmetry in the condylar angle and length of the condylar neck, and anomaly of occlusion resulting from many residual roots with deep caries. Mouth-opening and mandibular movements were normal, however, the presence of temporomandibular joint symptoms was unclear because of the patient’s unconsciousness at the time of the scan. The bifid mandibular condyle could have resulted from a bicycle accident when the patient was 7 years of age, based on information from the patient’s family.Isomura ET, Kobashi H, Tanaka S, Enomoto A, Kogo M (2017) A Case of Bifid Mandibular Condyle. OMICS J Radiol 6: 278. DOI: 10.4172/2167-7964.1000278

General anaesthesia with and without intubation for patients with Cornelia de Lange syndrome

We present the use of different methods of general anaesthesia in two patients with Cornelia de Lange syndrome and its contribution to the patients’ oral health.Case 1: The patient was a 22-year-old woman with Cornelia de Lange syndrome who underwent dental treatment under general anaesthesia. She exhibited the physical characteristics of Cornelia de Lange syndrome, including a small mouth, thin lips, short limbs, stiffness of joints and intellectual disability. General anaesthesia without intubation was performed safely eight times. No other complications except hypersensitivity to hypnotic agents were observed.Case 2: The patient was a 10-year-old boy with Cornelia de Lange syndrome who underwent dental treatment under general anaesthesia. He had a history and symptoms of obstructive airway disorders in addition to showing physical characteristics of the syndrome similar to those seen in Case 1. General anaesthesia with nasal intubation was performed safely twice. Computed tomography (CT) of his head and neck produced unremarkable results. These cases demonstrate that both general anaesthesia with and without nasal intubation can be safely used in managing individuals with Cornelia de Lange syndrome during dental treatment.Keywords: general anaesthesia; Cornelia de Lange syndrome; dental treatmen

The role of information and communications technology in real-time monitoring for quality WASH infrastructure

The risk of inefficiencies in quality of WASH infrastructure construction is higher in places where monitoring is less or absent or when monitoring is only limited to persons directly involved with the business processes and transactions. To address system deficiencies and constraints in the monitoring of latrine construction in WASH in schools’ project, CRS Ghana explored innovative technology solutions that allow access to quick, cost-effective quality data on progress of construction activities. The solution involves the use of Tablets, GPS, Iformbuilder Platform and BarTender software to develop a simple system to track and certify construction results in real-time. This approach prevents construction defects, supports cost-effective delivery of quality infrastructure, promotes transparency and allows decision making on payments, contracts and WASH programming

Reconstructing the Primordial Spectrum with CMB Temperature and Polarization

We develop a new method to reconstruct the power spectrum of primordial curvature perturbations, $P(k)$, by using both the temperature and polarization spectra of the cosmic microwave background (CMB). We test this method using several mock primordial spectra having non-trivial features including the one with an oscillatory component, and find that the spectrum can be reconstructed with a few percent accuracy by an iterative procedure in an ideal situation in which there is no observational error in the CMB data. In particular, although the previous ``cosmic inversion'' method, which used only the temperature fluctuations, suffered from large numerical errors around some specific values of $k$ that correspond to nodes in a transfer function, these errors are found to disappear almost completely in the new method.Comment: 18 pages, 17 figures, submitted to PR

Reconstructing the primordial power spectrum from the CMB

We propose a straightforward and model independent methodology for characterizing the sensitivity of CMB and other experiments to wiggles, irregularities, and features in the primordial power spectrum. Assuming that the primordial cosmological perturbations are adiabatic, we present a function space generalization of the usual Fisher matrix formalism, applied to a CMB experiment resembling Planck with and without ancillary data. This work is closely related to other work on recovering the inflationary potential and exploring specific models of non-minimal, or perhaps baroque, primordial power spectra. The approach adopted here, however, most directly expresses what the data is really telling us. We explore in detail the structure of the available information and quantify exactly what features can be reconstructed and at what statistical significance.Comment: 43 pages Revtex, 23 figure

DNA secondary structure is influenced by genetic variation and alters susceptibility to de novo translocation

<p>Abstract</p> <p><b>Background</b></p> <p>Cumulative evidence suggests that DNA secondary structures impact DNA replication, transcription and genomic rearrangements. One of the best studied examples is the recurrent constitutional t(11;22) in humans that is mediated by potentially cruciform-forming sequences at the breakpoints, palindromic AT-rich repeats (PATRRs). We previously demonstrated that polymorphisms of PATRR sequences affect the frequency of <it>de novo </it>t(11;22)s in sperm samples from normal healthy males. These studies were designed to determine whether PATRR polymorphisms affect DNA secondary structure, thus leading to variation in translocation frequency.</p> <p><b>Methods</b></p> <p>We studied the potential for DNA cruciform formation for several PATRR11 polymorphic alleles using mobility shift analysis in gel electrophoresis as well as by direct visualization of the DNA by atomic force microscopy. The structural data for various alleles were compared with the frequency of <it>de novo </it>t(11;22)s the allele produced.</p> <p><b>Results</b></p> <p>The data indicate that the propensity for DNA cruciform structure of each polymorphic allele correlates with the frequency of <it>de novo </it>t(11;22)s produced (r = 0.77, <it>P </it>= 0.01).</p> <p><b>Conclusions</b></p> <p>Although indirect, our results strongly suggest that the PATRR adopts unstable cruciform structures during spermatogenesis that act as translocation hotspots in humans.</p

Preserved acute pain and impaired neuropathic pain in mice lacking protein interacting with C Kinase 1

Protein interacting with C Kinase 1 (PICK1), a PDZ domain-containing scaffolding protein, interacts with multiple different proteins in the mammalian nervous system and is believed to play important roles in diverse physiological and pathological conditions. In this study, we report that PICK1 is expressed in neurons of the dorsal root ganglion (DRG) and spinal cord dorsal horn, two major pain-related regions. PICK1 was present in approximately 29.7% of DRG neurons, most of which were small-less than 750 μm2 in cross-sectional area. Some of these PICK1-positive cells co-labeled with isolectin B4 or calcitonin-gene-related peptide. In the dorsal horn, PICK1 immunoreactivity was concentrated in the superficial dorsal horn, where it was prominent in the postsynaptic density, axons, and dendrites. Targeted disruption of PICK1 gene did not affect basal paw withdrawal responses to acute noxious thermal and mechanical stimuli or locomotor reflex activity, but it completely blocked the induction of peripheral nerve injury-induced mechanical and thermal pain hypersensitivities. PICK1 appears to be required for peripheral nerve injury-induced neuropathic pain development and to be a potential biochemical target for treating this disorder

An Experimental Analysis to Determine the Load-Bearing Capacity of 3D Printed Metals

Copyright: © 2022 by the authors. Reverse engineering is conducted based on the analysis of an already existing product. The results of such an analysis can be used to improve the functioning of the product or develop new organizational, economic, information technology, and other solutions that increase the efficiency of the entire business system, in particular 3D printed products. Therefore, the main aim of this research is to focus on evaluation of the load-bearing capacity of already existing 3D printed metals in order to see their suitability for the intended application and to obtain their relevant mechanical properties. To this end, 3D printed metallic bars with almost square cross-sections were acquired from an external company in China without any known processing parameters, apart from the assumption that specimens No. 1–3 are printed horizontally, and specimens No. 4–7 are printed vertically. Various experiments were conducted to study microstructural characteristics and mechanical properties of 3D printed metals. It was observed that specimens No. 1–6, were almost similar in hardness, while specimen No. 7 was reduced by about 4.5% due to the uneven surface. The average value of hardness for the specimens was found to be approximately 450 HV, whereas the load-extension graphs assessed prior point towards the conclusion that the specimens’ fractured in a brittle status, is due to the lack of plastic deformation. For different specimens of the 3D printed materials, the main defects were identified, namely, lack of fusion and porosity are directly responsible for the cracks and layer delamination, prevalent in SLM printed metals. An extensive presence of cracks and layer delamination prove that the printing of these metallic bars was completed in a quick and inaccurate manner, which led to higher percentages of lack of fusion due to either low laser power, high scan speed, or the wrong scan strategy