Quantitative assay of non-specific esterases in the developing egg of Emerita asiatica

It is a well known fact that crustacean yolk contains a considerable quantity of storage lipids to be utilized during embryogenesis. Esterases are the main hydrolytic enzymes responsible for converting complex storage lipids into easily utilizable glycerides and free fatty acids. Esterases exist as isozymes which could be characterized histochemically after separating them on polyacrylamide gel

Identification and characterization of vitellogenin and lipovitellin of Scylla serrata and Emerita asiatica using disc gel electrophoresis

The appearance of a sex limited plasmatic protein (FSP) in the mature crabs and other higher crustaceans is now well established. As early as 1954, Frentz observed the FSP in the blood of Carcinus maenas during vitellogenesis. This protein is considered to be the precursor of the main yolk protein of the egg

Serological identification of vitellogenin and lipovitellin in Scylla serrata and Emerita asiatica using immuno electrophoresis

Vitellogenin is the blood protein precursor of lipovitellin, the main yolk protein. Many crustacean workers have used electrophoresis to detect the vitellogenin and lipovitellin in the blood and ovary respectively. However, authenticity of identical relative mobilities in homologizing vitellogenin with lipovitellin has been sometimes questioned because fluctuating current, buffer strength and gel composition may lead to variations in the relative mobility of the same component

Detection and characterization of esterase isozyme by disc gel electrophoresis using inhibitors

The non-specific esterases include different types of esterases,, acetyl, aryl, carboxyl and cholin esterases. These forms are identified on the basis of their differential activity towards various inorganic inhibitors (Holmes and Master, 1968 ; Dickinson and Johnson, 1978). Similarly isozymes of a type of esterase are detected by the differences in their molecular weight, as shown by their relative mobilities in disc gel electrophoresi

Spirometric Evaluation of Occupational Respiratory Dysfunction among Hospital Sanitary Workers in a Tertiary Care Centre

BACKGROUND: Working in dusty environment face the risk of inhaling particulate materials that may lead to adverse respiratory effects. Sanitary workers are exposed to a number of pathogens, toxic substances, chemicals that come from the waste itself and from its decomposition. As a result of their exposure to multiple risk factors, they suffer high rates of occupational health problems, which would definitely alter the pulmonary functions and respiratory endurances. Individuals who breathe through their mouth have higher pulmonary ventilation rates when comparing to those who breathe through their nose. This is likely to be attributed to the occupational exposure of this group to workplace contaminants, particularly bio aerosols. So this study was done to evaluate the respiratory dysfunction among sanitary workers who are exposed to environmental and occupational hazards. AIM OF THE STUDY: To evaluate the occupational respiratory dysfunction among sanitary workers. OBJECTIVES: 1. To evaluate the respiratory functions in sanitary workers and normal healthy individuals. 2. To compare the effect of duration of exposure on respiratory functions in sanitary workers. MATERIALS AND METHODS: A cross sectional study was done after IEC approval, with written informed consent on 120 individuals of age group 20-45 years. Group I- 60 sanitary workers involved in waste collection and disposal of both gender. GROUP II- 60 healthy non exposed candidates, age and gender matched. Detailed history and clinical examination was carried out to rule out any acute or chronic illness. Information regarding respiratory illness, frequency and symptoms noted. The pulmonary function parameters; Forced Vital Capacity [FVC], Forced Expiratory Volume in 1 second [FEV1], Forced Expiratory Flow [FEF25–75] Peak Expiratory Flow [PEF] were recorded using spirometer, according to the American Thoracic Society criteria. Parameters were compared using SPSS16.0 version. RESULTS: All pulmonary function parameters were reduced in sanitary workers compared to control group. FVC%, FEV1 was reduced significantly. CONCLUSION: The lung functions are commonly affected due to occupational exposure in sanitary workers

Experiments on yolk protein uptake in crustacean ovary

Recent electron microscopic and biochemical investigations have revealed the pinocytotic uptake of extra ovarian proteins into the vitellogenic oocytes (Hinsch and Cone, 1969; Wolin et at., 1973). The uptake of these macromolecular yolk precursor substances could be demonstrated using trypan blue as well as horse radish peroxidase

Non-Syndromic Solitary Neurofibroma in floor of the mouth: A case report

Neurofibromas are rare in the head and neck region, but most frequent tumor of neural origin. Oral hard and soft tissue is affected bythe tumor. In this paper, we describe an unusual case of non-syndromic solitary neurofibroma of the floor of the mouth in a 70-yearfemalepatient with a chief complaint of growth in the floor of the mouth for the past 3 months. An occlusal, intraoral periapicalradiograph and CT imaging were done. After confirming the diagnosis, the lesion was excised under local anesthesia and the specimenwas submitted for histopathological examination. On subsequent follow-up, the patient was asymptomatic. Intraoral neurofibromaalthough uncommon, deserve special attention because of their similarity with other inflammatory neoplastic condition, and theirtendency to undergo malignant transformation

A study on growth, feed efficiency and hematological changes in Pearlspot, Etroplus suratensis (Bloch, 1790) in response to varied salinities in raceway-based culture system

1571-1579A 60 days experiment was conducted in raceway tanks to determine the impact of salinity on growth, survival, carcass composition and hematology of Etroplus suratensis fingerlings. The experimental unit consisted of three treatments viz., T1 (0 ppt), T2 (15 ppt), and T3 (25 ppt). Complete randomized design (CRD) was followed with each treatment in triplicate. A total of 1500 fingerlings were stocked in each tank with an average body weight of 22.43±0.88 g. The results of the present experiment showed that fishes reared in 15 ppt salinities has showed an improved growth performance in terms of specific growth rate (0.35±0.01 % day-1), feed conversion ratio (0.11±0.01), feed efficiency ratio (8.72±0.52) and protein efficiency ratio (0.20±0.01), compared to 0 ppt and 25 ppt. Similarly, fishes reared in lower salinity (0 to 15 ppt) showed a better proximate composition compared to higher salinity of 25 ppt. In addition, fishes reared in 15 ppt salinity showed higher levels of hemoglobin (7.63±0.08 gm dl-1), red blood cell (1.6±0.12 million cum m-1), white blood cell (253000±57735 cells cum m-1) and packed cell volume (21.63±0.08 %). Likewise, low levels of platelet, mean corpuscular volume (135.80±0.32 Fl), mean corpuscular hemoglobin (48.53±0.17 pg) and mean corpuscular hemoglobin concentration (35.46±0.20 gm %)) were observed in 15 ppt reared fishes. From the current study it is concluded that E. suratensis can be acclimatized to different water salinity and thereby it can be suggested as a potential candidate for farming at 15 ppt salinity