Use of farm grown cereal/protein mixes by lactating dairy cows in a winter diet

We compared the use of three farm grown cereal/protein mixes (oat/fava bean, barley/lupin, triticale/pea), that were used to complement lactating dairy cow diets based on hay consisting of alfalfa/orchard grass and perennial grassland. The trial took place in 2007 at the INRA experimental station of Mirecourt, where an organic mixed-crop dairy system is being prototyped. The experiment was carried out in a Latin square with three groups of eight cows (50% Holstein, 50% Montbéliarde). The cows were fed 4 kg/cow/day of one of the three cereal/protein mixes, 8 kg DM/cow/day of alfalfa/orchardgrass hay, and permanent grassland hay ad libitum. The three diets were indicative of the animals’ needs. Diets had no significant effect on milk production (20.3 kg/cow/d) and milk fat content (41.3 g/kg). However, dairy cows fed with the oat/fababean mix had a significantly lower protein content in their milk. This is the result of: (i) a nitrogen surplus in relation to energy, and (ii) a lower starch content compared to the two other diets. Therefore, in organic mixed-crop dairy systems with forages that are rich in nitrogen, the choice to cultivate mixtures of cereal/pulses and the composition of these mixtures must be primarily based on their agronomic interest and not on their zootechnical one since they are not essential to a balanced animal diet

Weight management: a comparison of existing dietary approaches in a work-site setting

<b>OBJECTIVES:</b> (1) To compare the effectiveness a 2512 kJ (600 kcal) daily energy deficit diet (ED) with a 6279 kJ (1500 kcal) generalized low-calorie diet (GLC) over a 24 week period (12 weeks weight loss plus 12 weeks weight maintenance). (2) To determine if the inclusion of lean red meat at least five times per week as part of a slimming diet is compatible with weight loss in comparison with a diet that excludes lean red meat. DESIGN: Randomized controlled trial. <b>SETTING:</b> Large petrochemical work-site. <b>PARTICIPANTS:</b> One-hundred and twenty-two men aged between 18 and 55 y. <b>MAIN OUTCOME MEASURES:</b> Weight loss and maintenance of weight loss. <b>INTERVENTION:</b> Eligible volunteers were randomized to one of the four diet=meat combinations (ED meat, ED no meat, GLC meat, GLC no meat). One-third of subjects in each diet/meat combination were randomized to an initial control period prior to receiving dietary advice. All subjects attended for review every 2 weeks during the weight loss period. For the 12 week structured weight maintenance phase, individualized energy prescriptions were re-calculated for the ED group as 1.4 (activity factor)x basal metabolic rate. Healthy eating advice was reviewed with subjects in the GLC group. All subjects were contacted by electronic mail at 2 week intervals and anthropometric and dietary information requested. <b>RESULTS:</b> No difference was evident between diet groups in mean weight loss at 12 weeks (4.3 (s.d. 3.4) kg ED group vs 5.0 (s.d. 3.5) kg GLC group, P=0.34). Mean weight loss was closer to the intended weight loss in the 2512 kJ (600 kcal) ED group. The dropout rate was also lower than the GLC group. The inclusion of lean red meat in the diet on at least five occasions per week did not impair weight loss. Mean weight gain following 12 weeks weight maintenance was þ1.1 (s.d. 1.8) kg, P<0.0001. No differences were found between groups. <b>CONCLUSIONS:</b> This study has shown that the individualized 2512 kJ (600 kcal) ED approach was no more effective in terms of weight loss than the 6279 kJ (1500 kcal) GLC approach. However the ED approach might be considered preferable as compliance was better with this less demanding prescription. In terms of weight loss the elimination of red meat from the diet is unnecessary. The weight maintenance intervention was designed as a low-input approach, however weight regain was significant and weight maintenance strategies require further development

Visceral Adiposity Index: A reliable indicator of visceral fat function associated with cardiometabolic risk

Objective: To individuate a novel sex-specific index, based on Waist Circumference (WC), Body Mass Index (BMI), Triglycerides (TG) and HDL cholesterol (HDL), indirectly expressing visceral fat function. Research design and Methods: Visceral Adiposity Index (VAI) was first modelled on 315 non-obese healthy subjects. Using two multiple logistic regression models, VAI was retrospectively validated in 1,498 primary care patients in comparison to classical cardio and cerebrovascular risk factors. Results: All components of metabolic syndrome increased significantly across VAI quintiles. VAI was independently associated with both cardiovascular (OR:2.45; 95%CI: 1.52-3.95; p<0.001) and cerebrovascular events (OR:1.63; 95%CI: 1.06-2.50; p=0.025). VAI also showed significant inverse correlation with insulin sensitivity during euglycemic-hyperinsulinemic clamp in a subgroup of patients (R(s)= -0.721; p<0.001). By contrast, no correlations were found for WC and BMI. Conclusions: Our study suggests VAI is a valuable indicator of "visceral adipose function" and insulin sensitivity, and its increase is strongly associated with cardiometabolic risk

Hypertriglyceridemic waist identifies HIV+ men and women at increased cardiometabolic risk

Screening for increased waist circumference and hypertriglyceridemia (the hypertriglyceridemic-waist phenotype) is an inexpensive approach to identify patients at risk of coronary artery disease in apparently healthy individuals who may be at increased risk of type 2 diabetes and coronary heart disease because of an excess of intra-abdominal (visceral) fat. We examined the ralationship between the hypertriglyceridemic-waist and selected cardiometabolic risk factors in HIV individuals

Human in vitro reporter model of neuronal development and early differentiation processes

<p>Abstract</p> <p>Background</p> <p>During developmental and adult neurogenesis, doublecortin is an early neuronal marker expressed when neural stem cells assume a neuronal cell fate. To understand mechanisms involved in early processes of neuronal fate decision, we investigated cell lines for their capacity to induce expression of doublecortin upon neuronal differentiation and develop <it>in vitro </it>reporter models using doublecortin promoter sequences.</p> <p>Results</p> <p>Among various cell lines investigated, the human teratocarcinoma cell line NTERA-2 was found to fulfill our criteria. Following induction of differentiation using retinoic acid treatment, we observed a 16-fold increase in doublecortin mRNA expression, as well as strong induction of doublecortin polypeptide expression. The acquisition of a neuronal precursor phenotype was also substantiated by the establishment of a multipolar neuronal morphology and expression of additional neuronal markers, such as Map2, βIII-tubulin and neuron-specific enolase. Moreover, stable transfection in NTERA-2 cells of reporter constructs encoding fluorescent or luminescent genes under the control of the doublecortin promoter allowed us to directly detect induction of neuronal differentiation in cell culture, such as following retinoic acid treatment or mouse Ngn2 transient overexpression.</p> <p>Conclusion</p> <p>Induction of doublecortin expression in differentiating NTERA-2 cells suggests that these cells accurately recapitulate some of the very early events of neuronal determination. Hence, the use of reporter genes under the control of the doublecortin promoter in NTERA-2 cells will help us to investigate factors involved early in the course of neuronal differentiation processes. Moreover the ease to detect the induction of a neuronal program in this model will permit to perform high throughput screening for compounds acting on the early neuronal differentiation mechanisms.</p

Expert improvisers in Western classical music learning pathways

Despite a growing interest in Western classical improvisation among researchers, educators and musicians in recent decades, research insights on expert improvisers' learning pathways are scarce. In order to further understanding this phenomenon, we formulated the following research question: “What characterizes the learning pathways of Western classical music expert improvisers?” Addressing this question, we designed an exploratory case study, conducting open-ended semi-structured videoconference interviews with a purposeful sampling of N = 8 Western classical music expert improvisers. The participants are international classically trained musicians who are recognized as expert improvisers by their peers and who have improvised on professional albums and in established concert halls. In-depth analysis of our data revealed two distinct learning pathways among the participants: (1) native improvisers, who have improvised since the very beginning of their instrumental learning; and (2) immigrant improvisers, who started to improvise at a later age, during their graduate studies or at the beginning of their professional career. Native improvisers began to improvise spontaneously, without apparent extrinsic incentive, while immigrant improvisers started to improvise in order to attempt to fill a gap in their musical practice. Various factors motivated the immigrant improvisers interviewed to themselves dedicate to this practice, including seeing improvisation as a means to experience (i) a ‘getting back’ to oneself; (ii) an authentic human encounter; (iii) a sense of immediacy characterizing the creative process; and (iv) an equalitarian musical practice. Lastly, a ‘learn-unlearn’ process appears to underlie improvisational expertise development. Implications of these findings for expertise development and skill acquisition will be discussed

Atomistic Simulations of Nanotube Fracture

The fracture of carbon nanotubes is studied by atomistic simulations. The fracture behavior is found to be almost independent of the separation energy and to depend primarily on the inflection point in the interatomic potential. The rangle of fracture strians compares well with experimental results, but predicted range of fracture stresses is marketly higher than observed. Various plausible small-scale defects do not suffice to bring the failure stresses into agreement with available experimental results. As in the experiments, the fracture of carbon nanotubes is predicted to be brittle. The results show moderate dependence of fracture strength on chirality.Comment: 12 pages, PDF, submitted to Phy. Rev.

Towards Verification of Unstructured-Grid Solvers

New methodology for verification of computational methods using unstructured grids is presented. The discretization order properties are studied in computational windows, easily constructed within a collection of grids or a single grid. The windows can be adjusted to isolate the interior discretization, the boundary discretization, or singularities. A major component of the methodology is the downscaling test, introduced previously for studying the convergence rates of truncation and discretization errors of finite-volume discretization schemes on general unstructured grids. Demonstrations of the method are shown, including a comparative accuracy assessment of commonly-used schemes on general mixed grids and the identification of local accuracy deterioration at intersections of tangency and inflow/outflow boundaries. Recommendations for the use of the methodology in large-scale computational simulations are given

Forging Resilience to HIV/AIDS: Personal Strengths of Middle-aged and Older Gay, Bisexual, and Other Men Who Have Sex With Men Living With HIV/AIDS

HIV-positive gay, bisexual, two-spirit, and other men who have sex with men (MSM) have exhibited significant resilience to HIV/AIDS in Canada since the start of the epidemic. Since 2012, most of the research that has been conducted on resilience to HIV/AIDS has utilized quantitative methods and deficits-based approaches, with a preferential focus on the plight of young MSM. In order to address apparent gaps in research on HIV/AIDS resilience, we conducted a community-based participatory research qualitative study that utilized a strengths-based approach to examine the perspectives and lived experiences of HIV-positive, middle-aged and older MSM on their individual attributes that helped forge their HIV/AIDS resilience. We conducted 41 semistructured interviews with diverse, HIV-positive, middle-aged and older MSM from Central and Southwestern Ontario, Canada. From our thematic analysis of our interviews, we identified four themes, which represented personal strengths that fostered resilience to HIV/AIDS: (a) proactiveness, (b) perseverance, (c) having the right mindset, and (d) self-awareness with self-control. This article discusses the importance of these personal strengths to fostering HIV/AIDS resilience, and how community-based resources could potentially lessen the need to muster such personal strengths, or alternatively, cultivate them

Protective Antiviral Immunity Conferred by a Nonintegrative Lentiviral Vector-Based Vaccine

Lentiviral vectors are under intense scrutiny as unique candidate viral vector vaccines against tumor and aggressive pathogens because of their ability to initiate potent and durable specific immune responses. Strategies that alleviate safety concerns will facilitate the clinical developments involving lentiviral vectors. In this respect, the development of integration deficient lentiviral vectors circumvents the safety concerns relative to insertional mutagenesis and might pave the way for clinical applications in which gene transfer is targeted to non-dividing cells. We thus evaluated the potential use of nonintegrative lentiviral vectors as vaccination tools since the main targeted cell in vaccination procedures is the non-dividing dendritic cell (DC). In this study, we demonstrated that a single administration of nonintegrative vectors encoding a secreted form of the envelope of a virulent strain of West Nile Virus (WNV) induces a robust B cell response. Remarkably, nonintegrative lentiviral vectors fully protected mice from a challenge with a lethal dose of WNV and a single immunization was sufficient to induce early and long-lasting protective immunity. Thus, nonintegrative lentiviral vectors might represent a safe and efficacious vaccination platform for the development of prophylactic vaccines against infectious agents