Facilitating the adoption of high-throughput sequencing technologies as a plant pest diagnostic test in laboratories: A step-by- step description

High-throughput sequencing (HTS) is a powerful tool that enables the simultaneous detection and potential identification of any organisms present in a sample. The growing interest in the application of HTS technologies for routine diagnostics in plant health laboratories is triggering the development of guidelines on how to prepare laboratories for performing HTS testing. This paper describes general and technical recommendations to guide laboratories through the complex process of preparing a laboratory for HTS tests within existing quality assurance systems. From nucleic acid extractions to data analysis and interpretation, all of the steps are covered to ensure reliable and reproducible results. These guidelines are relevant for the detection and identification of any plant pest (e.g. arthropods, bacteria, fungi, nematodes, invasive plants or weeds, protozoa, viroids, viruses), and from any type of matrix (e.g. pure microbial culture, plant tissue, soil, water), regardless of the HTS technology (e.g. amplicon sequencing, shotgun sequencing) and of the application (e.g. surveillance programme, phytosanitary certification, quarantine, import control). These guidelines are written in general terms to facilitate the adoption of HTS technologies in plant pest routine diagnostics and enable broader application in all plant health fields, including research. A glossary of relevant terms is provided among the Supplementary Material

Joint Optimal Design and Operation of Hybrid Energy Storage Systems

The wide range of performance characteristics of storage technologies motivates the use of a hybrid energy storage systems (HESS) that combines the best features of multiple technologies. However, HESS design is complex, in that it involves the choice of storage technologies, the sizing of each storage element, and deciding when to charge and discharge each underlying storage element (the operating strategy.We formulate the problem of jointly optimizing the sizing and the operating strategy of an HESS that can be used for a large class of applications and storage technologies. Instead of a single set of storage element sizes, our approach determines the Pareto-optimal frontier of the sizes of the storage elements along with the corresponding optimal operating strategy. Thus, as long as the performance objective of a storage application (such as an off-grid microgrid) can be expressed as a linear combination of the underlying storage sizes, the optimal vector of storage sizes falls somewhere on this frontier. We present two case studies to illustrate our approach, demonstrating that a single storage technology is sometimes inadequate to meet application requirements, unlike an HESS designed using our approach. We also find simple, near-optimal, and practical operating strategies for these case studies, which allows us to gain several new engineering insights

Methane emissions and the use of Desmanthus in beef cattle production in Northern Australia

The Australian beef industry is a major contributor to the economy with an estimated annual revenue generation of over seven billion dollars. The tropical state of Queensland accounted for 48% of Australian beef and veal production in 2018. As the third biggest beef exporter in the world, Australia supplies 3% of the world’s beef exports and its agricultural sector accounts for an estimated 13.2% of its total greenhouse gas emissions. About 71% of total agricultural emissions are in the form of methane and nitrous oxide. In this review, an overview of the carbon footprint of the beef cattle production system in northern Australia is presented, with emphasis on the mitigation of greenhouse gases. The review also focuses on the tropical legume, Desmanthus, one of the more promising nutritional supplements for methane abatement and improvement of animal growth performance. Among the review’s findings is the need to select environmentally well-adapted and vigorous tropical legumes containing tannins that can persistently survive under the harsh northern Australian conditions for driving animal performance, improving meat quality and reducing methane emissions. The paper argues that the use of appropriate legumes such as Desmanthus, is a natural and preferred alternative to the use of chemicals for the abatement of methane emanating from tropical beef cattle production systems. It also highlights current gaps in knowledge and new research opportunities for in vivo studies on the impact of Desmanthus on methane emissions of supplemented tropical beef cattle

Plasma metabolites, productive performance and rumen volatile fatty acid profiles of Northern Australian Bos indicus steers supplemented with Desmanthus and lucerne

The hypothesis tested was that tropical steers supplemented with the Desmanthus legume and lucerne, a widely characterized temperate legume of high nutritive value, would elicit similar responses in plasma metabolite profiles, productive performance, nitrogen retention, and volatile fatty acids (VFA). The tannin-binding compound, polyethylene glycol-4000 (PEG), was added to the diets (160 g/kg Desmanthus dry matter) with the objective of further exploring nitrogen (N) utilization in the animals supplemented with Desmanthus relative to lucerne. From February to June 2020, sixteen yearling Brangus steers (average liveweight of 232 ± 6 kg) were fed a background diet of Rhodes grass (Chloris gayana) hay for 28 days, before introducing three Desmanthus cultivars (Desmanthus virgatus cv. JCU2, D. bicornutus cv. JCU4, D. leptophyllus cv. JCU7) and lucerne (Medicago sativa) at 30% dry matter intake (DMI). Relative to the backgrounding period, all supplemented steers exhibited similar growth performance. Steers supplemented with Desmanthus recorded a lower DMI and animal growth performance, but higher fecal N concentration than animals supplemented with lucerne. Among the three Desmanthus cultivars, there were no significant differences in N concentrations, VFA, and plasma metabolite profiles. The addition of PEG induced higher rumen iso-acid concentrations and fecal N excretion. However, feeding Desmanthus spp. to tropical Bos indicus steers could be a valuable means of increasing N utilization, which is attributable to the presence of tannins, and, consequently, improve animal productive performance. Since supplementation with lucerne resulted in higher liveweight, daily liveweight gains, and overall animal performance than supplementing with Desmanthus, the tested hypothesis that both supplements will elicit similar animal performance does not hold and must be rejected. Further in vivo investigation is needed to better understand the impact of tannins in Desmanthus on N utilization

A randomized controlled trial of subcutaneous nerve stimulation for back pain due to failed back surgery syndrome: the SubQStim study

Objectives: To compare the effectiveness of peripheral nerve stimulation utilizing a subcutaneous lead implant technique—subcutaneous nerve stimulation (SQS) plus optimized medical management (SQS + OMM arm) vs. optimized medical management alone (OMM arm) in patients with back pain due to failed back surgery syndrome. Patients and Methods: Patients were recruited from 21 centers, in Europe, Israel, and Australia. Eligible patients were randomized (1:1) to SQS + OMM or OMM arms. Those in the SQS arm were implanted with a neurostimulator and up to two subcutaneous percutaneous cylindrical leads in the area of pain. Patients were evaluated pre‐randomization and at one, three, six, and nine months post‐randomization. The primary endpoint was the proportion of subjects with a ≥50% reduction in back pain intensity (“responder”) from baseline to nine months. Secondary outcomes included proportion of responders with a ≥50% reduction in back pain intensity at six months and ≥30% reduction at nine months, and the mean change from baseline in back pain intensity at six and nine months between the two arms. Results: Due to the slow rate of recruitment, the study was terminated early with 116 subjects randomized. A total of 33.9% (19/56, missing: n = 20 [36%]) of subjects in the SQS + OMM arm and 1.7% (1/60, missing: n = 24 [40%]) in the OMM arm were responders at Month 9 (p < 0.0001). Secondary objectives showed a significant difference in favor of SQS + OMM arm. Conclusion: The results indicate that the addition of SQS to OMM is more effective than OMM alone in relieving low back pain at up to nine months

Angular distribution of photons from the delay of the GDR in hot and rotating light Yb nuclei from exclusive experiments

Abstract Angular distributions of photons associated with the damping of excited-state giant dipole resonances (GDR) in hot and rotating 161,162 Yb nuclei have been measured in exclusive experiments using the HECTOR array. In reactions with heavy ions ( 48 T) angular distributions are determined as a function of the angular momentum of the compound nuclei. In reactions with lighter ions ( 17,18 O) a difference method is applied to isolate GDR decays originating from specific excitation regions. The systematics of the measured angular distributions as a function of excitation energy and angular momentum are compared to theories taking into account fluctuations of the shape and orientation of the excited nuclei

The response to flexibility: country intervention choices in the first four rounds of the GAVI Health Systems Strengthening applications

Since December 2005 the GAVI Alliance (GAVI) Health Systems Strengthening (HSS) window has offered predictable funding to developing countries, based on a combined population and economic formula. This is intended to assist them to address system constraints to improved immunization coverage and health care delivery, needed to meet the Millennium Development Goals. The application process invites countries to prioritize specific system constraints not adequately addressed by other donors, and allows them to allocate their eligible funds accordingly. This article presents an analysis of the first four rounds of countries' funding applications. These requested funding for a variety of health system initiatives that reflected country-specific requirements, and were not limited to improving immunization coverage. Analyses identified a dominance of operational-level health service provision activities, and an absence of interventions related to demand and financing. While the proposed activities are only now being implemented, the results of this study provide evidence that the open application process employed by the HSS window has led to a shift in analysis and planning—from the programmatic to the systemic—in the countries whose applications have been approved. However, the proposed responses to identified constraints are dominated by short-term operational responses, rather than more complex, longer term approaches to health system strengthenin

The GDR width in the excited 147 Eu compound nucleus at high angular momentum

Abstract High-energy γ -rays emitted in the decay of the hot compound nucleus 147 Eu have been measured in coincidence with low-energy γ -rays. The γ transitions from the different residual nuclei were detected by a multiplicity filter and by Ge detectors. The employed reaction was 37 Cl + 110 Pd at bombarding energies of 160, 165 and 170 MeV. The measured high-energy γ -ray spectra were analysed within the framework of the statistical model using the Cascade code. The GDR width in the angular momentum interval between 35 and 50 ℏ was found to increase weakly and to be rather well predicted by the thermal shape fluctuation model. Also the deformation parameter β as a function of the average angular momentum extracted from the data was found to be in general agreement with the model

Measurement of 15 MeV γ-rays with the Ge cluster detectors of EUROBALL

A measurement of the response to 15.1 MeV c-rays has been made for the Ge cluster detectors in the EUROBALL array. Each cluster detector consists of seven germanium capsules surrounded by a single anticompton shield of BGO. The reaction D(11B,c)12C# na tE "%!. "19.1 MeV has been employed. The 'adding-backa of signals simultaneously present in the capsules composing each cluster detector has been made on an event by event basis. The intensity in full-energy peak increases by a factor of three as compared to that of the spectrum obtained by summing the individual spectra of the 7 capsules. The pulse height to energy conversion is found to be very linear from few hundreds keV to 15 MeV. The e$ciency is discussed relative to that of large volume BaF 2 scintillators. ( 2000 Elsevier Science B.V. All rights reserved

Defective STIM-mediated store operated Ca2+ entry in hepatocytes leads to metabolic dysfunction in obesity

Defective Ca2+ handling is a key mechanism underlying hepatic endoplasmic reticulum (ER) dysfunction in obesity. ER Ca2+ level is in part monitored by the store-operated Ca2+ entry (SOCE) system, an adaptive mechanism that senses ER luminal Ca2+ concentrations through the STIM proteins and facilitates import of the ion from the extracellular space. Here, we show that hepatocytes from obese mice displayed significantly diminished SOCE as a result of impaired STIM1 translocation, which was associated with aberrant STIM1 O-GlycNAcylation. Primary hepatocytes deficient in STIM1 exhibited elevated cellular stress as well as impaired insulin action, increased glucose production and lipid droplet accumulation. Additionally, mice with acute liver deletion of STIM1 displayed systemic glucose intolerance. Conversely, over-expression of STIM1 in obese mice led to increased SOCE, which was sufficient to improve systemic glucose tolerance. These findings demonstrate that SOCE is an important mechanism for healthy hepatic Ca2+ balance and systemic metabolic control