4,947 research outputs found
Modelling diverse root density dynamics and deep nitrogen uptake — a simple approach
We present a 2-D model for simulation of root density and plant nitrogen (N) uptake for crops grown in agricultural systems, based on a modification of the root density equation originally proposed by Gerwitz and Page in J Appl Ecol 11:773–781, (1974). A root system form parameter was introduced to describe the distribution of root length vertically and horizontally in the soil profile. The form parameter can vary from 0 where root density is evenly distributed through the soil profile, to 8 where practically all roots are found near the surface. The root model has other components describing root features, such as specific root length and plant N uptake kinetics. The same approach is used to distribute root length horizontally, allowing simulation of root growth and plant N uptake in row crops. The rooting depth penetration rate and depth distribution of root density were found to be the most important parameters controlling crop N uptake from deeper soil layers. The validity of the root distribution model was tested with field data for white cabbage, red beet, and leek. The model was able to simulate very different root distributions, but it was not able to simulate increasing root density with depth as seen in the experimental results for white cabbage. The model was able to simulate N depletion in different soil layers in two field studies. One included vegetable crops with very different rooting depths and the other compared effects of spring wheat and winter wheat. In both experiments variation in spring soil N availability and depth distribution was varied by the use of cover crops. This shows the model sensitivity to the form parameter value and the ability of the model to reproduce N depletion in soil layers. This work shows that the relatively simple root model developed, driven by degree days and simulated crop growth, can be used to simulate crop soil N uptake and depletion appropriately in low N input crop production systems, with a requirement of few measured parameters
Motor Performance as Risk Factor for Lower Extremity Injuries in Children
Purpose: Physical activity related injuries in children constitute a costly public health matter. The influence of motor performance on injury risk is unclear. The purpose was to examine if motor performance was a risk factor of traumatic and overuse lower extremity injuries in a normal population of children.
Methods: This study included 1244 participants from 8 to 14-years-old at baseline, all participating in "the Childhood Health, Activity and Motor Performance School Study Denmark". The follow-up period was up to 15 months. The motor performance tests were static balance, single leg hop for distance, core stability tests, vertical jump, shuttle run, and a cardiorespiratory fitness test. Lower extremity injuries were registered by clinicians by weekly questionnaires and classified according to the ICD-10 system.
Results: Poor balance increased risk for traumatic injury in the foot region (IRR=1.09-1.15), and good performance in single leg hop for distance protected against traumatic knee injuries (IRR=0.66-0.68). Good performance in core stability tests and vertical jump increased the risk for traumatic injuries in the foot region (IRR=1.12-1.16). Poor balance increased the risk for overuse injuries in the foot region (IRR=1.65), as did good performance in core stability tests and shuttle run, especially for knee injuries (IRR=1.07-1.18).
Conclusions: Poor balance (sway) performance was a consistent predictor of traumatic injuries, in particular for traumatic ankle injuries. Good motor performance (core stability, vertical jump, shuttle run) was positively associated with traumatic and overuse injuries, and negatively (single leg hop) associated with traumatic injuries, indicating different influence on injury risk. Previous injury was a confounder affecting the effect size and the significance. More studies are needed to consolidate the findings, to clarify the influence of different performance tests on different types of injuries and to examine the influence of behaviour in relation to injury ris
Recombination and selectional forces in cyanopeptolin NRPS operons from highly similar, but geographically remote Planktothrix strains
<p>Abstract</p> <p>Background</p> <p>Cyanopeptolins are nonribosomally produced heptapetides showing a highly variable composition. The cyanopeptolin synthetase operon has previously been investigated in three strains from the genera <it>Microcystis</it>, <it>Planktothrix </it>and <it>Anabaena</it>. Cyanopeptolins are displaying protease inhibitor activity, but the biological function(s) is (are) unknown. Cyanopeptolin gene cluster variability and biological functions of the peptide variants are likely to be interconnected.</p> <p>Results</p> <p>We have investigated two cyanopeptolin gene clusters from highly similar, but geographically remote strains of the same genus. Sequencing of a nonribosomal peptide synthetase (NRPS) cyanopeptolin gene cluster from the Japanese strain <it>Planktothrix </it>NIES 205 (205-<it>oci</it>), showed the 30 kb gene cluster to be highly similar to the <it>oci </it>gene cluster previously described in <it>Planktothrix </it>NIVA CYA 116, isolated in Norway. Both operons contained seven NRPS modules, a sulfotransferase (S) and a glyceric acid loading (GA)-domain. Sequence analyses showed a high degree of conservation, except for the presence of an epimerase domain in NIES 205 and the regions around the epimerase, showing high substitution rates and Ka/Ks values above 1. The two strains produce almost identical cyanopeptolins, cyanopeptolin-1138 and oscillapeptin E respectively, but with slight differences regarding the production of minor cyanopeptolin variants. These variants may be the result of relaxed adenylation (A)-domain specificity in the nonribosomal enzyme complex. Other genetic markers (16S rRNA, <it>ntc</it>A and the phycocyanin <it>cpc</it>BA spacer) were identical, supporting that these geographically separated <it>Planktothrix </it>strains are closely related.</p> <p>Conclusion</p> <p>A horizontal gene transfer event resulting in exchange of a whole module-encoding region was observed. Nucleotide statistics indicate that both purifying selection and positive selection forces are operating on the gene cluster. The positive selection forces are acting within and around the epimerase insertion while purifying selection conserves the remaining (major) part of the gene cluster. The presence of an epimerase in the gene cluster is in line with the D-configuration of Htyr, determined experimentally in oscillapeptin E in a previous study.</p
[OI]63micron jets in class 0 sources detected by Herschel
We present Herschel PACS mapping observations of the [OI]63 micron line
towards protostellar outflows in the L1448, NGC1333-IRAS4, HH46, BHR71 and
VLA1623 star forming regions. We detect emission spatially resolved along the
outflow direction, which can be associated with a low excitation atomic jet. In
the L1448-C, HH46 IRS and BHR71 IRS1 outflows this emission is kinematically
resolved into blue- and red-shifted jet lobes, having radial velocities up to
200 km/s. In the L1448-C atomic jet the velocity increases with the distance
from the protostar, similarly to what observed in the SiO jet associated with
this source. This suggests that [OI] and molecular gas are kinematically
connected and that this latter could represent the colder cocoon of a jet at
higher excitation. Mass flux rates (\.M(OI)) have been measured from
the [OI]63micron luminosity adopting two independent methods. We find values in
the range 1-4 10 Mo/yr for all sources but HH46, for which an order of
magnitude higher value is estimated. \.M(OI) are compared with mass
accretion rates (\.M) onto the protostar and with \.M derived
from ground-based CO observations. \.M(OI)/\.M ratios are in
the range 0.05-0.5, similar to the values for more evolved sources.
\.M(OI) in HH46 IRS and IRAS4A are comparable to \.M(CO), while
those of the remaining sources are significantly lower than the corresponding
\.M(CO). We speculate that for these three sources most of the mass
flux is carried out by a molecular jet, while the warm atomic gas does not
significantly contribute to the dynamics of the system.Comment: 37 pages and 12 figures, accepted for publication on Astrophysical
Journa
Bias and temperature dependence of the 0.7 conductance anomaly in Quantum Point Contacts
The 0.7 (2e^2/h) conductance anomaly is studied in strongly confined, etched
GaAs/GaAlAs quantum point contacts, by measuring the differential conductance
as a function of source-drain and gate bias as well as a function of
temperature. We investigate in detail how, for a given gate voltage, the
differential conductance depends on the finite bias voltage and find a
so-called self-gating effect, which we correct for. The 0.7 anomaly at zero
bias is found to evolve smoothly into a conductance plateau at 0.85 (2e^2/h) at
finite bias. Varying the gate voltage the transition between the 1.0 and the
0.85 (2e^2/h) plateaus occurs for definite bias voltages, which defines a gate
voltage dependent energy difference . This energy difference is
compared with the activation temperature T_a extracted from the experimentally
observed activated behavior of the 0.7 anomaly at low bias. We find \Delta =
k_B T_a which lends support to the idea that the conductance anomaly is due to
transmission through two conduction channels, of which the one with its subband
edge \Delta below the chemical potential becomes thermally depopulated as the
temperature is increased.Comment: 9 pages (RevTex) with 9 figures (some in low resolution
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