Next-to-leading order corrections to Bhabha scattering in renormalization group approach (I). Soft and virtual photonic contributions

Soft and virtual loop photonic contributions to the second order next-to-leading QED radiative corrections to Bhabha scattering are calculated with help of the renormalization group approach. The results are in agreement with earlier calculations, where other methods were used. Scale dependence and the present theoretical accuracy of Bhabha cross section description are discussed.Comment: 12 pages, 10 Figures, elsart.cl

Application ofthe lucentis in patient with disease Best

The article presents a case of a favorable outcome of the disease Best in patients treated with “Lucentis”. This reduced the progression of choroidal neovascularization and thereby stabilize visual functions.В статье представлен случай благоприятного исхода болезни Беста у пациента, получившего лечение препаратом «Луцентис». Это позволило уменьшить прогрессирование хориоидальной неоваскуляризации и тем самым добиться стабилизации зрительных функций

Infectious bursal disease virus: identification of the novel genetic group and reassortant viruses

The results of the phylogenic analysis of the nucleotide sequence of the IBDV A and B genome segments have been presented. Traditionally the IBDV isolates are classified based on the phylogenic analysis of the hypervariable region of the VP2 gene. The analysis of the VP2 gene segments of the isolates detected in the Russian Federation demonstrated that most of them belong to the genetic group comprising highly virulent IBDV isolates. However, not all isolates belonging to one genetic group have the same phenotypic characteristics. This is related to the fact that the virulence is determined not only based on the characteristics of the VP2 gene (A segment) but on the characteristics of the VP1 gene (B segment) as well. The IBDV genome segmentation allows formation of reassortant viruses which can be identified as a result of the genome segment analysis. The phylogenic analysis of the nucleotide sequences of VP2 and VP1 genes of 28 IBDV isolates detected at RF, Ukrainian and Kazakh poultry establishments in 2007 and 2019 showed that 15 of them are reassortant viruses. Different combinations of the genome segments have been identified among these reassortant viruses. Detection of different combinations of IBDV genome segments is indicative of the fact that the heterogeneous virus population circulates on the poultry farms. Pathogenicity studies of the three IBDV isolates showed that the most virulent was an isolate having two genome segments characteristic of the highly virulent virus. Two reassortant viruses having only one genome segment A or B, characteristic of the infectious bursal disease, demonstrated less pronounced virulent properties

Enrichment of field crops biodiversity in conditions of climate changing

Gradual introduction into the culture of new species requires introduction of appropriate technologies of their cultivation, with taking into account varietal characteristics, species adaptation to the conditions of cultivation, analysis of yield and quality formation characteristics, taking into account content of biologically valuable components, depending on technological factors and environmental factors. The dynamics of the weather changes in the Right-bank Forest-steppe of Ukraine during the period is analyzed 2004-2017. The analysis of weather conditions showed a tendency the air temperature increasing and decrease of rainfall amount during period of spring crops vegetation compare with the average annual data. Weather conditions had their own peculiarities, sometimes they were extreme, which adversely affected on growth, development and productivity of plants was noted a tendency of increasing amount of active and effective temperatures during the growing season, what necessitates expansion of field crops biodiversity, especially spring crops. Identified cultures: lentil (Lens culinaris), chick-pea (Cicer arietinum), chufa (Camelina sativa); triticale (Triticale); millet (Panicum miliaceum); sorghum (Sorghum bicolor); white mustard (Sinapis alba); brown mustard (Brassica juncea); coriander (Coriandrum sativum) that, due to biological and technological characteristics, are suitable for introduction into production. Installation of the elements of adaptive technologies for the cultivation of scarce field crops. Developed elements of adaptive cultivating technology for species, introducted into the field culture, contribute to reducing the influence of uncontrolled factors on productivity formation, the role of species increasing, sustainable development of formed agrocenoses, improving the quality of raw materials and food safety. Analysis of weather conditions and the correspondence of biological characteristics of rare cultures in Ukraine have allowed distinguish cultures that have high production efficiency

New data on the level of immune stratum against Q fever agent in population of the of Republic of Guinea

which is bacteria of the species Coxiella burnetii. One of the factors showing the possibility of pathogen circulation in a certain territory is assessed by the presence of an immune stratum in the inhabitants of the region. In the 1980s, the study of the immune structure of the population of the Republic of Guinea in relation to coxiellosis has begun. The present study, carried out in 2015—2019, has been aimed to obtain new information about the immune stratum of the population of the Republic of Guinea against the causative agent of Q fever and to compare it with previous studies. Specific IgG antibodies in the blood of the Guinea residents were detected by using enzyme-linked immunosorbent assay (ELISA) with a set of reagents manufactured at the St. Petersburg Pasteur Institute (St. Petersburg, Russian Federation). The serum samples were tested in at 1:100 dilution. Antibodies against C. burnetii were detected in 124/2346 (5.3% [CI 4.5-6.3]) samples. This study confirms the previously obtained data on the circulation of the causative agent of coxiellosis in all landscape and geographical zones of the Republic of Guinea. The natural and climatic conditions of the region, the variety of ixodic tick species currently inhabiting this territory being a reservoir and vector of infection, as well as a large amount of livestock are the factors for active circulation of the Q fever pathogen and the emergence of related disease outbreaks. The data obtained necessitate continuing further studies on distribution of C. burnetii in the territory of the Republic of Guinea. Taking into consideration the epidemiological significance of Q fever, a pressing task is to study a proportion of this infectious disease in the overall structure of diseases registered in the territory of the Republic of Guinea. It is also necessary to conduct regular epizootological monitoring in order to clarify the types of carriers and vectors of C. burnetii in different landscape and geographical zones of the Republic of Guinea as well as to assess the immune stratum against the pathogen in large and small cattle being the main sources of infection for humans. The data obtained will allow us to determine presence of a natural focus of this infection as well as its borders and develop a set of preventive (anti-epidemic) measures

Infidelity of SARS-CoV Nsp14-Exonuclease Mutant Virus Replication Is Revealed by Complete Genome Sequencing

Most RNA viruses lack the mechanisms to recognize and correct mutations that arise during genome replication, resulting in quasispecies diversity that is required for pathogenesis and adaptation. However, it is not known how viruses encoding large viral RNA genomes such as the Coronaviridae (26 to 32 kb) balance the requirements for genome stability and quasispecies diversity. Further, the limits of replication infidelity during replication of large RNA genomes and how decreased fidelity impacts virus fitness over time are not known. Our previous work demonstrated that genetic inactivation of the coronavirus exoribonuclease (ExoN) in nonstructural protein 14 (nsp14) of murine hepatitis virus results in a 15-fold decrease in replication fidelity. However, it is not known whether nsp14-ExoN is required for replication fidelity of all coronaviruses, nor the impact of decreased fidelity on genome diversity and fitness during replication and passage. We report here the engineering and recovery of nsp14-ExoN mutant viruses of severe acute respiratory syndrome coronavirus (SARS-CoV) that have stable growth defects and demonstrate a 21-fold increase in mutation frequency during replication in culture. Analysis of complete genome sequences from SARS-ExoN mutant viral clones revealed unique mutation sets in every genome examined from the same round of replication and a total of 100 unique mutations across the genome. Using novel bioinformatic tools and deep sequencing across the full-length genome following 10 population passages in vitro, we demonstrate retention of ExoN mutations and continued increased diversity and mutational load compared to wild-type SARS-CoV. The results define a novel genetic and bioinformatics model for introduction and identification of multi-allelic mutations in replication competent viruses that will be powerful tools for testing the effects of decreased fidelity and increased quasispecies diversity on viral replication, pathogenesis, and evolution

ПОЛИМОРФИЗМ ГЕНА ТР53 У ПАЦИЕНТОВ С РАКОМ ЖЕЛУДКА В ПОПУЛЯЦИОННОМ ПРОСПЕКТИВНОМ И КЛИНИЧЕСКОМ ИССЛЕДОВАНИЯХ

Background. A functionally significant TP53Arg72Pro polymorphism can contribute to the development of gastric cancer (GC).The aim: to study the associations of genotypes and alleles of the TP53Arg72Pro 4 polymorphism with GC and biomarkers of gastric ucosal atrophy in population-based prospective and case-control clinical trials among the population of Siberia.Material and methods. As a part of the epidemiological study, data of the international multicenter HAPIEE project for 2003–05, based on a population sample of residents of Novosibirsk city (serum and  DNA samples) and data of the population-based registry of GC  (2012) were compared. Gastric cancer patients were matched by  age and sex to HAPIEE population controls. A total of 156 serum  samples (GC – 52, control – 104) and 146 DNA samples (GC – 50,  control – 96) were available for prospective analysis. DNA samples  from 80 gastric cancer patients (45 men and 35 women, mean age  61.0 ± 13.4 years) and from 87 age-and sex-matched controls were  analyzed. DNA samples from venous blood were genotyped  according to standard methods. Serum samples were tested using  diagnostic kit for enzyme-linked immunosorbent assays to determine the levels of pepsinogen I (PGI), PGII, PGI/PGII ratio, gastrin-17 and IgG antibodies to H. pylori.Results. No differences in genotype and allele frequencies of the TP53 gene between the case group and the control group were  found. A decreased frequency of the Pro allele in female gastric  cancer patients compared with controls indicated that the Pro allele  is protective against the development of gastric cancer, but this  effect was not observed in male patients. No associations of TP53  genotypes with the risk of diffuse or intestinal gastric cancer, as well  as with the age and sex of patients were found. A high frequency of  genotypes with the Pro allele in patients with stage III–IV gastric  cancer indicated the relationship between Arg/Pro TR53 and tumor  progression, in particular, the contribution of the minor Pro allele to  the unfavorable prognosis. A prospective study showed high risk of  reducing the level of pepsinogen for assessing predisposition to  gastric cancer.Conclusion. Two case-control studies (population and clinical) conducted in the Western Siberia found no relationship between the  TP53Arg72Pro polymorphism and the risk of gastric cancer. However, the TP53 genotype with a rare Pro allele was associated with atrophic gastritis and severity of gastric cancer.Актуальность. Функционально значимый полиморфизм Arg72Pro 4 экзона гена онкосупрессора ТР53 может вносить вклад в развитие рака желудка (РЖ).Цель исследования – изучить ассоциации генотипов и аллелей полиморфизма Arg72Pro 4  экзона гена ТР53 с РЖ и биомаркерами атрофии слизистой у пациентов с РЖ в популяционном проспективном и клиническом исследованиях «случай-контроль» в сибирской популяции.Материал и методы. В рамках эпидемиологического исследования были сопоставлены  база международного многоцентрового проекта HAPIEE, сформированная в 2003–05 гг. на  популяционной выборке жителей Новосибирска (образцы ДНК и сыворотки крови хранились при –70 ºС), и данные популяционного регистра РЖ (2012 г). Для каждого случая РЖ в  соотношении 1:2 был подобран соответствующий по возрасту и полу контроль из базы  HAPIEE. 156 образцов сыворотки крови (РЖ – 52, контроль – 104) и 146 образцов ДНК (РЖ – 50, контроль – 96) оказались доступны для проспективного анализа. В клинической группе  проанализирована серия случаев РЖ (45 мужчин и 35 женщин, средний возраст – 61,0 ±  13,4 годы) из 2 специализированных лечебных учреждений. В качестве «контроля» из базы  HAPIEE были использованы образцы ДНК 87 человек, подобранных к «случаям» по полу и  возрасту. Образцы ДНК из венозной крови генотипировали по стандартным методикам.  Образцы сыворотки тестировали с помощью диагностикумов для иммуноферментного анализа с определением уровней пепсиногена I (ПГI), ПГII,  соотношения ПГI/ПГII, гастрина-17 и IgG антител к H. рylori.Результаты. Частоты генотипов и аллелей гена ТР53 у пациентов с РЖ в обоих исследованиях не различались, как и между группами «случай» и «контроль».  Снижение частоты аллеля Pro у женщин с РЖ по сравнению с контролем дает основания  рассматривать Pro аллель в качестве защитного в отношении формирования рака желудка.  Для мужчин такой закономерности не отмечено. Не обнаружено связи генотипов ТР53 с  риском развития диффузного или интестинального типов рака желудка, а также ассоциаций  с возрастом и полом пациентов. Высокая частота генотипов с аллелем Pro у больных РЖ III– IV стадии свидетельствует о возможном влиянии генетического статуса ТР53 по Arg/Pro полиморфизму на опухолевую прогрессию, в частности, вклад минорного аллеля Pro в  неблагоприятный прогноз. В проспективном исследовании получены данные о высокой  рисковой значимости снижения уровня пепсиногена для оценки предрасположенности к раку желудка.Выводы. В двух исследованиях «случай-контроль» (популяционном и клиническом) в  регионе Западной Сибири не найдено связи полиморфизма Arg72Pro гена ТР53 с риском  развития рака желудка. Однако генотип ТР53 с редким аллелем Pro ассоциирован с  атрофическим гастритом, который является предраковым состоянием, а также c клиническим течением РЖ