Changes in health-related behaviours and mental health in a UK public sample during the first set of COVID-19 public health restrictions

Public health restrictions, in response to the COVID-19 pandemic, have had potentially wide-ranging, unintended effects on health-related behaviours such as diet and physical activity and also affected mental health due to reduced social interactions. This study explored how health-related behaviours and mental health were impacted in a sample of the UK public during the COVID-19 pandemic. Two online surveys were administered in the UK, one within the first three months of the restrictions (Timepoints 1 (involving pre-pandemic recall) and 2) and another ten weeks later (Timepoint 3). Moderate-vigorous physical activity (MVPA), sitting time, screen time and sexual activity were self-reported. Diet was assessed using the Dietary Instrument for Nutri-tion Education questionnaire. Mental health was measured using the short-form Warwick-Edinburgh Mental Well-being Scale and Becks’ Anxiety and Depression Inventories. Differences between timepoints were explored using the Friedman, Wilcoxon signed-rank, McNemar and McNemar-Bowker tests. 296 adults (74% under 65 years old; 65% female) provided data across all timepoints. Between T1 and T2, MVPA, time outdoors and sexual activity decreased while sitting and screen time increased (p<0.05). Between T2 and T3, saturated fat intake, MVPA, time out-doors, and mental wellbeing increased while sitting, screen time and anxiety symptoms decreased (p<0.05). This study found that depending on the level of COVID-19 public health restrictions in place, there appeared to be a varying impact on different health-related behaviours and mental health. As countries emerge from restrictions, it will be prudent to direct necessary resources to address these important public health issues

Reversion of an E-coli strain carrying an IS1-activated bgl operon under nonselective conditions is predominantly due to deletions within the structural genes

The bgl operon of Escherichia coli, which encodes the genes necessary for transport and catabolism of beta-glucosides, is silent in wild-type cells and is activated by the transposition of IS elements. The silent form of the operon appears to be the stable state. We isolated Bgl(-) revertants of an activated strain after growth under nonselective conditions to understand whether activation of the cryptic operon by IS elements is reversible. Genetic and molecular analyses revealed that a majority of revertants carry deletions of the bgl structural genes, indicating that an irreversible alteration has occurred in the operon. Implications of these results for the evolution and maintenance of cryptic genes are discussed

Association between phospholipid metabolism in plasma and spontaneous preterm birth: a discovery lipidomic analysis in the cork pregnancy cohort

Introduction: Preterm birth (PTB) is defined as birth occurring before 37 weeks’ gestation, affects 5–9% of all pregnancies in developed countries, and is the leading cause of perinatal mortality. Spontaneous preterm birth (sPTB) accounts for 31–50% of all PTB, but the underlying pathophysiology is poorly understood. Objective: This study aimed to decipher the lipidomics pathways involved in pathophysiology of sPTB. Methods: Blood samples were taken from SCreening fOr Pregnancy Endpoints (SCOPE), an international study that recruited 5628 nulliparous women, with a singleton low-risk pregnancy. Our analysis focused on plasma from SCOPE in Cork. Discovery profiling of the samples was undertaken using liquid chromatography-mass spectrometry Lipidomics, and features significantly altered between sPTB (n = 16) and Control (n = 32) groups were identified using empirical Bayes testing, adjusting for multiple comparisons. Results: Twenty-six lipids showed lower levels in plasma of sPTB compared to controls (adjusted p < 0.05), including 20 glycerophospholipids (12 phosphatidylcholines, 7 phosphatidylethanolamines, 1 phosphatidylinositol) and 6 sphingolipids (2 ceramides and 4 sphingomyelines). In addition, a diaglyceride, DG (34:4), was detected in higher levels in sPTB compared to controls. Conclusions: We report reduced levels of plasma phospholipids in sPTB. Phospholipid integrity is linked to biological membrane stability and inflammation, while storage and breakdown of lipids have previously been implicated in pregnancy complications. The contribution of phospholipids to sPTB as a cause or effect is still unclear; however, our results of differential plasma phospholipid expression represent another step in advancing our understanding of the aetiology of sPTB. Further work is needed to validate these findings in independent pregnancy cohorts