Binding effects in multivalent Gibbs-Donnan equilibrium

The classical Gibbs-Donnan equilibrium describes excess osmotic pressure associated with confined colloidal charges embedded in an electrolyte solution. In this work, we extend this approach to describe the influence of multivalent ion binding on the equilibrium force acting on a charged rod translocating between two compartments, thereby mimicking ionic effects on force balance during in vitro DNA ejection from bacteriophage. The subtle interplay between Gibbs-Donnan equilibrium and adsorption equilibrium leads to a non-monotonic variation of the ejection force as multivalent salt concentration is increased, in qualitative agreement with experimental observations

Viral capsids: Mechanical characteristics, genome packaging and delivery mechanisms

The main functions of viral capsids are to protect, transport and deliver their genome. The mechanical properties of capsids are supposed to be adapted to these tasks. Bacteriophage capsids also need to withstand the high pressures the DNA is exerting onto it as a result of the DNA packaging and its consequent confinement within the capsid. It is proposed that this pressure helps driving the genome into the host, but other mechanisms also seem to play an important role in ejection. DNA packaging and ejection strategies are obviously dependent on the mechanical properties of the capsid. This review focuses on the mechanical properties of viral capsids in general and the elucidation of the biophysical aspects of genome packaging mechanisms and genome delivery processes of double-stranded DNA bacteriophages in particular

Raw data_ChO mechanics

GFP-expressing chordotonal organs were imaged during larval crawling in a linear agarose channel. The contour of the cap and ligament cells was followed over time. The original stream acquisitions are provided as multi-tiff files (prc.zip and wt.zip).Larval locomotion was followed for wild-type, pericardin-mutant and atonal-mutant larvae. We are happy to provide the original time lapse movies, however, the movie size for a single experiment is 1GB and we have 27 movies in total. We provide the results of the analysis (position and body angle for each time frame) as excel files

Raw Data_Noise reduction

Mechanical interaction was measured by monitoring fluorescent beads embedded in the substrate and performing digital image correlation analysis to obtain the displacement field and the strain field generated by a beating cardiac cell. We are happy to provide the original time lapse movies, however, the movie size for a single cell experiment is at least 10GB (The size of a time lapse of a cell interacting with a probe is 2GB and we use at least 5 interaction parameters). For the experiments of mechanical interaction between mechanically-coupled cardiac cells, each measurement is a 2GB time lapse movie. Our data consists of n=34 cells in the mechanical-probe assay and additional n=30 cells in the mechanically-coupled cardiac cell assay

Raw data_ChO mechanics

GFP-expressing chordotonal organs were imaged during larval crawling in a linear agarose channel. The contour of the cap and ligament cells was followed over time. The original stream acquisitions are provided as multi-tiff files (prc.zip and wt.zip).Larval locomotion was followed for wild-type, pericardin-mutant and atonal-mutant larvae. We are happy to provide the original time lapse movies, however, the movie size for a single experiment is 1GB and we have 27 movies in total. We provide the results of the analysis (position and body angle for each time frame) as excel files.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV

Your Automated Implantable Cardioverter Defibrillator Is Not a Bulletproof Vest but It Might Save Your Life

A 43-year-old male was brought to the emergency department as the highest level trauma activation with complaints of chest and arm pain after sustaining gunshot wounds (GSW). Initial workup was notable for superficial GSWs to the left chest and upper extremity with direct impact to the patient’s automated implantable cardioverter defibrillator. The patient underwent replacement of the device without rewiring and was discharged home without complications

“My Brain Can Stop”: An ERP Study of Longitudinal Prediction of Inhibitory Control in Adolescence

We examined the longitudinal predictors of electrophysiological and behavioral markers of inhibitory control in adolescence. Participants were 63 adolescent boys who have been followed since birth as part of a prospective longitudinal study on the developmental pathways to attention-deficit hyperactivity disorder (ADHD). At 17 years of age, they completed the stop-signal task (SST) while electroencephalography (EEG) was continuously recorded. Inhibitory control was evaluated by the stop-signal reaction time (SSRT) as well as by the amplitude of the event-related potential (ERP) component of N2 during successful inhibition. We found that higher inattention symptoms throughout childhood predicted reduced amplitude (i.e., less negative) of the N2 in adolescence. Furthermore, the N2 amplitude was longitudinally predicted by the early precursors of child familial risk for ADHD and early childhood temperament. Specifically, father’s inattention symptoms (measured in the child’s early infancy) and child’s effortful control at 36 months of age directly predicted the N2 amplitude in adolescence, even beyond the consistency of inattention symptoms throughout development. The SSRT was predicted by ADHD symptoms throughout childhood but not by the early precursors. Our findings emphasize the relevance of early familial and temperamental risk for ADHD to the prediction of a later dysfunction in inhibitory control

“My Brain Can Stop”: An ERP Study of Longitudinal Prediction of Inhibitory Control in Adolescence

We examined the longitudinal predictors of electrophysiological and behavioral markers of inhibitory control in adolescence. Participants were 63 adolescent boys who have been followed since birth as part of a prospective longitudinal study on the developmental pathways to attention-deficit hyperactivity disorder (ADHD). At 17 years of age, they completed the stop-signal task (SST) while electroencephalography (EEG) was continuously recorded. Inhibitory control was evaluated by the stop-signal reaction time (SSRT) as well as by the amplitude of the event-related potential (ERP) component of N2 during successful inhibition. We found that higher inattention symptoms throughout childhood predicted reduced amplitude (i.e., less negative) of the N2 in adolescence. Furthermore, the N2 amplitude was longitudinally predicted by the early precursors of child familial risk for ADHD and early childhood temperament. Specifically, father’s inattention symptoms (measured in the child’s early infancy) and child’s effortful control at 36 months of age directly predicted the N2 amplitude in adolescence, even beyond the consistency of inattention symptoms throughout development. The SSRT was predicted by ADHD symptoms throughout childhood but not by the early precursors. Our findings emphasize the relevance of early familial and temperamental risk for ADHD to the prediction of a later dysfunction in inhibitory control