1,656 research outputs found

    Unsupervised Content Based Image Retrieval by Combining Visual Features of an Image With A Threshold

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    Content-based image retrieval (CBIR) uses the visual features of an image such as color, shape and texture to represent and index the image. In a typical content based image retrieval system, a set of images that exhibit visual features similar to that of the query image are returned in response to a query. CLUE (CLUster based image rEtrieval) is a popular CBIR technique that retrieves images by clustering. In this paper, we propose a CBIR system that also retrieves images by clustering just like CLUE. But, the proposed system combines all the features (shape, color, and texture) with a threshold for the purpose. The combination of all the features provides a robust feature set for image retrieval. We evaluated the performance of the proposed system using images of varying size and resolution from image database and compared its performance with that of the other two existing CBIR systems namely UFM and CLUE. We have used four different resolutions of image. Experimentally, we find that the proposed system outperforms the other two existing systems in ecery resolution of imag

    Optimization of callus induction and regeneration system for Pakistani wheat cultivars Kohsar and Khyber-87

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    Wheat is a member of family Poaceae. It is the major staple food of Pakistan. The present study was done to improve the regeneration of two commercially grown wheat varieties Kohsar and Khyber-87.Mature embryos were used as explants. Five different concentrations of 2,4-D; 2, 2.5, 3, 3.5 and 4 mg/L were used for callus induction. For regeneration, initially different concentrations (0.1 to 0.2) of IAA(indole-3-acetic acid) and BAP (6-benzylaminopurine) were experimented. The best combination of these hormones that is, 0.1 mg/L IAA and 0.5 mg/L BAP were further subjected to experimentation along with different concentrations of kinetin; 0.1, 0.2, 0.3, 0.4, 0.5 and 1 mg/L. Maximum calli of Kohsar (83.3%) was obtained at 3 mg/L 2,4-D whereas for Khyber-87 maximum callus induction (71.70%) was obtained at 3.5 mg/l 2,4-D. The maximum regeneration of both Kohsar and Khyber-87 (80.5 and 62.2%, respectively) were obtained at the combinations of 0.1 mg/L IAA, 0.5 mg/L BAP and 0.5 mg/L kinetin

    Phytotoxicity assessment of untreated and biotreated hospital wastewater on crop plants

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    BCDDO: Binary Child Drawing Development Optimization

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    This study presents the vectorization of metaheuristic algorithms as the first stage of vectorized optimization implementation. Vectorization is a technique for converting an algorithm, which operates on a single value at a time to one that operates on a collection of values at a time to execute rapidly. The vectorization technique also operates by replacing multiple iterations into a single operation, which improves the algorithm's performance in speed and makes the algorithm simpler and easier to be implemented. It is important to optimize the algorithm by implementing the vectorization technique, which improves the program's performance, which requires less time and can run long-running test functions faster, also execute test functions that cannot be implemented in non-vectorized algorithms and reduces iterations and time complexity. Converting to vectorization to operate several values at once and enhance algorithms' speed and efficiency is a solution for long running times and complicated algorithms. The objective of this study is to use the vectorization technique on one of the metaheuristic algorithms and compare the results of the vectorized algorithm with the algorithm which is non-vectorized.Comment: 13 page

    An overview of enhancing drought tolerance in cotton through manipulating stress resistance genes

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    Drought stress affects the normal growth of plant by influencing Physiological, morphological molecular and biochemical traits at cellular level. It is a polygenic trait, controlled by multiple genes, which makes its manipulation difficult by genetic engineering. It seems drought could be major threat in future to high yield of cotton in Pakistan as well around the globe because it is spontaneous and cannot be controlled with manuring and skilled agricultural practices. Gene manipulation could be a solution of this threat by producing transgenic cotton plants. As it is polygenic trait, so, understanding about cellular mechanism of drought tolerance is crucial to impart tolerance by controlling gene expression under stressed conditions. Universal Stress Proteins (USP) genes have already been identified in drought stressed leaves of Gossypium arboreum which make this variety of cotton a rich source of stress tolerance genes. USP genes could be manipulated for drought tolerant transgenic cotton with high yielding as well and it is most important family of proteins in this regard. This family encompasses a conserved group of proteins that has been reported in different organisms which are activating under various abiotic stress conditions. USP is also a regulatory protein; its activity can be increased by manipulating its interactions

    Machine Learning Approach to Predict Quality Parameters for Bacterial Consortium-Treated Hospital Wastewater and Phytotoxicity Assessment on Radish, Cauliflower, Hot Pepper, Rice and Wheat Crops

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    Raw hospital wastewater is a source of excessive heavy metals and pharmaceutical pol-lutants. In water-stressed countries such as Pakistan, the practice of unsafe reuse by local farmers for crop irrigation is of major concern. In our previous work, we developed a low-cost bacterial consortium wastewater treatment method. Here, in a two-part study, we first aimed to find what physico-chemical parameters were the most important for differentiating consortium-treated and untreated wastewater for its safe reuse. This was achieved using a Kruskal–Wallis test on a suite of physico-chemical measurements to find those parameters which were differentially abundant between consortium-treated and untreated wastewater. The differentially abundant parameters were then input to a Random Forest classifier. The classifier showed that ‘turbidity’ was the most influential parameter for predicting biotreatment. In the second part of our study, we wanted to know if the consortium-treated wastewater was safe for crop irrigation. We therefore carried out a plant growth experiment using a range of popular crop plants in Pakistan (Radish, Cauliflower, Hot pepper, Rice and Wheat), which were grown using irrigation from consortium-treated and untreated hospital wastewater at a range of dilutions (turbidity levels) and performed a phytotoxicity assessment. Our results showed an increasing trend in germination indices and a decreasing one in phytotoxicity indices in plants after irrigation with consortium-treated hospital wastewater (at each dilution/turbidity measure). The comparative study of growth between plants showed the following trend: Cauliflower > Radish > Wheat > Rice > Hot pepper. Cauliflower was the most adaptive plant (PI: −0.28, −0.13, −0.16, −0.06) for the treated hospital wastewater, while hot pepper was susceptible for reuse; hence, we conclude that bacterial consortium-treated hospital wastewater is safe for reuse for the irrigation of cauliflower, radish, wheat and rice. We further conclude that turbidity is the most influential parameter for predicting bio-treatment efficiency prior to water reuse. This method, therefore, could represent a low-cost, low-tech and safe means for farmers to grow crops in water stressed areas

    Quality of life of road traffic injury survivors aged 15-44 years suffered from moderate and severe head injuries in Karachi, Pakistan

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    Background: Most of Road traffic injuries are predicted to be the third leading contributor to the global burden of disease by 2020 and patients end up into head injuries which leave devastating impacts on individual and society. Quality of Life After Brain Injury (QOLIBRI) is a specific tool that measures quality of life in head injury patients.Methods: This was a cross sectional study among 300 patients aged 15-45 years, attended emergency department of the Aga khan University Hospital. Structured questionnaire included demography, injury details, QOLIBRI, GOSE and WHO Disability Assessment Scale (WHODAS 12 items). Severity of injury was determined by Glasgow coma score of subjects reported in emergency department on admission. This was one time telephonic survey in which bidirectional data was collected from subjects about injury and outcome (QOLIBRI). To identify the determinants of QOL among RTI survivors with head injury multiple linear regressions was applied.Results: The mean QOL score for study sample was 69.86 ± (15.89. Most of the patients 210 (70%) were motorbike rider followed by four-wheelers 69 (23%). Mean age of RTI participants was 28.10 ± (7.68) years. The quality of life decreases with increase in severity of head injury by -697.32 (95% CI; -1006.44, -388.20).Conclusions: This study suggests severity of head injury, recovery time, surgery resulting from RTA, employment, family system and disability to have long term impact on QOL of RTI survivors

    Prevalence of hepatitis B in the blood donors of NW. F.P and FATA regions and the current scenario of HBV infection in Pakistan

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    Hepatitis B is a fatal liver disease caused by the hepatitis B virus. In this study, blood donors from various districts of the North-western frontier province and the federally administered tribal area (FATA) of Pakistan were tested for HBsAg and HBV DNA by ICT (Immuno-chromatographic test), ELISA and RTPCR. Out of the 7148 blood donors, 244 (3.41%) were positive for HBsAg by ICT, 147 (2.05%) by ELISA while 132 (1.85%) were positive by PCR. Our data indicates that the incidence of hepatitis B has decreased in these regions in recent times.Key words: HBV, HBsAg, Pakistan

    EFFICACY AND SAFETY OF ACETAMINOPHEN COMPARED TO TRAMADOL AFTER LAPAROSCOPIC CHOLECYSTECTOMY

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    Abstract Background: Laparoscopic cholecystectomy become widely popular because of its less postoperative pain produced compared to conventional cholecystectomy. The use of opioids postoperatively to reduce pain may be harmful and produce some adverse effects .Tramadol is a centrally acting opioid which is effective for postoperative analgesic pain but produce undesirable adverse effects the patients do not tolerate it so that intravenous acetaminophen could be helpful to optimize postoperative analgesia by reducing the requirement for opioids and their adverse effects.Method: A prospective randomized clinical trial was carried out on 27 adults undergoing elective laparoscopic cholecystectomy were randomly assigned to two groups in this study (13patients) to receive intravenously 1000mg acetaminophen and (14 patients) to receive intramuscularly tramadol 100mg postoperatively; visual analog scale (VAS) was utilized to asses pain severity and efficacy of analgesic agents. Pain scores were assessed at 0,2,4,6,8,10 and 12h after surgery the incidence of adverse effects was also assessed.Results: Over a period of 12hours, visual analog scale (VAS) scores were significantly lower in each group at each time interval compared to zero time; there is a non significant difference in pain score between the different groups; the incidence of adverse effects like nausea and vomiting was significant in tramadol group compared to acetaminophen group.Conclusion: Postoperative intravenous acetaminophen infusion of 1000mg is useful for reducing pain scores after elective laparoscopic cholecystectomy; it is effective and safe analgesic with less adverse effects compared to tramadol.  Key words: laparoscopic cholecystectomy, Acetaminophen, Tramadol, Postoperative analgesia.  Â

    Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat ( Triticum aestivum ) cultivars

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    Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation. For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated. For regeneration initially different concentrations of Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) were tested. Best hormone combinations were further subjected to Kinetin and 6-\u3b3-\u3b3-dimethylallylaminopurine (2iP). For Inqilab-91, Chakwal-97 and Manthar, 3 mg/L of 2,4-D was found optimum, which induced 83.25%, 77.75% and 95.20% of embryogenic calli, respectively. Maximum callus induction (97.18%) was observed in Tatara when 2 mg/L of 2,4-D was used. As regard to regeneration, Inqilab-91, Chakwal-97 and Manthar showed maximum regeneration on media containing 0.1 mg/L IAA, 0.4 mg/L Kinetin and 0.5 mg/L 2iP, regenerating 87.25%, 81.75% and 68.75% respectively. For Tatara maximum regeneration of 12.25% was obtained on 0.1 mg/L IAA and 2 mg/L of BAP. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crop
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