Long-Term Radio Modulation in Sagittarius A* from Spin-Induced Disk Precession

There is some evidence, though yet unconfirmed, that Sagittarius A*--the supermassive black hole at the Galactic center--emits its radio waves modulated with a ~100-day period. What is intriguing about this apparent quasi-periodicity is that, though the amplitude of the modulation increases with decreasing wavelength (from 3.6 to 1.3 cm), the quasi-period itself does not seem to depend on the frequency of the radiation. It is difficult to imagine how a binary companion, were that the cause of this modulation, could have escaped detection until now. Instead, it has been suggested that the spin-induced precession of a disk surrounding a slowly rotating black hole could have the right period to account for this behavior. In this paper, we examine how Sagittarius A*'s light curve could be modulated by this mechanism. We demonstrate that the partial occultation of a nonthermal halo by a compact, radio-opaque disk does indeed produce the observed frequency-dependent amplitude. This appears to be in line with other observational arguments suggesting that Sagittarius A*'s mm/sub-mm spectrum is produced by a ~10 Schwarzschild-radius disk, whereas its cm-waves originate from a nonthermal particle distribution in a halo extending out to over 20 Schwarzschild radii. Interestingly, this model suggests that the observed period corresponds to half the precession period and that a non-axisymmetric disk could produce a second period roughly twice as long as the first.Comment: 17 pages, 5 figures, accepted to Ap

Применение импульсных электронных пучков в качестве дезинфицирующего агента при обработке сточных вод

Background and Objective: Concerning current clinical practice, laser-assisted lipoplasty is still secondary to other procedures. In order to evaluate effects of thermal interaction with fatty-tissue, a near infrared diode laser was examined under reproducible conditions. Methods: Based on optical spectroscopy of fatty-tissue, a high-powered diode laser (lambda = 940 nm) was used to irradiate n = 59 fat samples of fresh corpses in non-contact mode. Thermal effects were histologically evaluated by computer based metric measurements. Calculated values included ablation rate (AR) and the ratio of cavity diameter to diameter of collateral damage (CCDratio). Pearson's correlation and analysis of covariance (ANCOVA) were used for statistical evaluation. P values of less than 0.05 were considered to indicate statistical significance. Results: Regarding the conditions examined, irradiances from 250 to 400 W/cm(2) revealed both increased ablation capacities and decreased collateral damages. An average irradiance of 370 +/- 0 W/cm(2) shows an average CCDratio of 2:1 and an average AR of 9.98 +/- 7.65 mm(3)/second. Conclusion: Near infrared high-powered diode laser energy proved to be eligible for tissue protective ablation of fat in vitro. Further studies are necessary to improve efficiency and safety of this procedure

Isomeric triazines exhibit unique profiles of bioorthogonal reactivity.

Expanding the scope of bioorthogonal reactivity requires access to new and mutually compatible reagents. We report here that 1,2,4-triazines can be tuned to exhibit unique reaction profiles with biocompatible strained alkenes and alkynes. Computational analyses were used to identify candidate orthogonal reactions, and the predictions were experimentally verified. Notably, 5-substituted triazines, unlike their 6-substituted counterparts, undergo rapid [4 + 2] cycloadditions with a sterically encumbered strained alkyne. This unique, sterically controlled reactivity was exploited for dual bioorthogonal labeling. Mutually orthogonal triazines and cycloaddition chemistries will enable new multi-component imaging applications

Pressure, stress, and strain distribution in the double-stage diamond anvil cell

Double stage diamond anvil cells (DAC) of two designs have been assembled and tested. We used a standard symmetric DAC as a primary stage and CVD microanvils machined by a focused ion beam - as a second. We evaluated pressure, stress, and strain distributions in Au and Fe-Au samples as well as in secondary anvils using synchrotron x-ray diffraction with a micro-focused beam. A maximum pressure of 240 GPa was reached independent of the first stage anvil culet size. We found that the stress field generated by the second stage anvils is typical of conventional DAC experiments. The maximum pressures reached are limited by strains developing in the secondary anvil and by cupping of the first stage diamond anvil in the presented experimental designs. Also, our experiments show that pressures of several megabars may be reached without sacrificing the first stage diamond anvils

Low angular momentum flow model of Sgr A* activity

Sgr A* is the closest massive black hole and can be observed with the highest angular resolution. Nevertheless, our current understanding of the accretion process in this source is very poor. The inflow is almost certainly of low radiative efficiency and it is accompanied by a strong outflow and the flow is strongly variable but the details of the dynamics are unknown. Even the amount of angular momentum in the flow is an open question. Here we argue that low angular momentum scenario is better suited to explain the flow variability. We present a new hybrid model which describes such a flow and consists of an outer spherically symmetric Bondi flow and an inner axially symmetric flow described through MHD simulations. The assumed angular momentum of the matter is low, i.e. the corresponding circularization radius in the equatorial plane of the flow is just above the innermost stable circular orbit in pseudo-Newtonian potential. We compare the radiation spectrum from such a flow to the broad band observational data for Sgr A*.Comment: Proceedings of the AHAR 2008 Conference: The Universe under the Microscope; Astrophysics at High Angular Resolution, Bad Honef

Sialic Acid Glycobiology Unveils Trypanosoma cruzi Trypomastigote Membrane Physiology.

Trypanosoma cruzi, the flagellate protozoan agent of Chagas disease or American trypanosomiasis, is unable to synthesize sialic acids de novo. Mucins and trans-sialidase (TS) are substrate and enzyme, respectively, of the glycobiological system that scavenges sialic acid from the host in a crucial interplay for T. cruzi life cycle. The acquisition of the sialyl residue allows the parasite to avoid lysis by serum factors and to interact with the host cell. A major drawback to studying the sialylation kinetics and turnover of the trypomastigote glycoconjugates is the difficulty to identify and follow the recently acquired sialyl residues. To tackle this issue, we followed an unnatural sugar approach as bioorthogonal chemical reporters, where the use of azidosialyl residues allowed identifying the acquired sugar. Advanced microscopy techniques, together with biochemical methods, were used to study the trypomastigote membrane from its glycobiological perspective. Main sialyl acceptors were identified as mucins by biochemical procedures and protein markers. Together with determining their shedding and turnover rates, we also report that several membrane proteins, including TS and its substrates, both glycosylphosphatidylinositol-anchored proteins, are separately distributed on parasite surface and contained in different and highly stable membrane microdomains. Notably, labeling for α(1,3)Galactosyl residues only partially colocalize with sialylated mucins, indicating that two species of glycosylated mucins do exist, which are segregated at the parasite surface. Moreover, sialylated mucins were included in lipid-raft-domains, whereas TS molecules are not. The location of the surface-anchored TS resulted too far off as to be capable to sialylate mucins, a role played by the shed TS instead. Phosphatidylinositol-phospholipase-C activity is actually not present in trypomastigotes. Therefore, shedding of TS occurs via microvesicles instead of as a fully soluble form

A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors

Glycans can be imaged by metabolic labeling with azidosugars followed by chemical reaction with imaging probes; however, tissue-specific labeling is difficult to achieve. Here we describe a strategy for the use of a caged metabolic precursor that is activated for cellular metabolism by enzymatic cleavage. An N-azidoacetylmannosamine derivative caged with a peptide substrate for the prostate-specific antigen (PSA) protease was converted to cell-surface azido sialic acids in a PSA-dependent manner. The approach has applications in tissue-selective imaging of glycans for clinical and basic research purposes. © 2010 American Chemical Society