7 research outputs found
DSP based current controlled single stage single phase integrated converter with external compensating signal for Class-C & Class-D appliances
Design and implementation of a current controlled single stage single phase integrated AC/DC isolated Power Factor Correction (PFC) converter is presented in this paper. With the integrated topology reduces the number control switches. The proposed converter has the advantage of low bulk capacitor voltage and only single control switch hence reduce in complexity in control and cost. Sub-harmonic oscillations which are produced in conventional current controller. By adding an external compensating signal effect of oscillations are reduced and performance of the converter is improved. The proposed scheme is implemented in real time by TMS320F2812 digital signal processor (DSP) board. The performance of converter is verified both experimentally and by simulation at different load and line conditions. The proposed converter is designed for 90–230 V, 50 Hz AC input, 48 V DC output and operating at 100 kHz switching frequency. The Experimental results shows that the DSP-based fuzzy controlled single phase single switch integrated PFC converter achieve high power factor and satisfies IEC-61000-3-2 and other European input current harmonic limits for Class-C & Class-D applications
Human aldosterone synthase gene polymorphism promotes miRNA binding and regulates gene expression
Strategies to Identify Common Low Effect COPD Risk Variants
Rationale: Chronic Obstructive Pulmonary Disease (COPD) is a common progressive lung disease. Risk for COPD is determined by an intersection between cigarette smoke inhalation and genetic predisposition. As with other disease risks determined by complex genetics, most variants thus far identified to be associated with COPD risk are common (variant allele frequency ≥0.05) and typically have low effect. Thus, after conclusion of multiple COPD Genome Wide Association Studies (GWAS) comprising thousands of subjects, most variants discovered account for less than 1% and the total accounts for less than 10% of variation in risk. Similarly sized GWAS that employ stringent control for false discovery do not have sufficient power to identify the common very low effect variants hypothesized to account for remaining variation in risk. Because larger GWAS are limited by economics and logistics, there is a need to develop strategies that increase the power to identify common very low effect COPD risk variants. There is mounting evidence that risk SNPs are located within regions responsible for cis-regulation of transcription. Our hypothesis is that false discovery will be reduced by restricting analysis to variants that a) are in putative cis-regulatory regions of high prior likelihood gene pathways, b) replicate for COPD association across multiple GWAS, and c) are experimentally confirmed to have cis-regulatory function.
Methods: COPD GWAS datasets phs000335 (Lung Health Study [LHS]) and phs000765 (COPDgene) were downloaded from the database of Genotypes and Phenotypes (dbGaP). Each dataset comprised subjects with primarily European ancestry. We restricted these datasets to putative cis-regulatory variants within 900 genes in pathways that have high prior likelihood for role in COPD causation based on literature, then assessed for replication across GWAS after sub-phenotyping as chronic bronchitis (CB) or emphysema (E).
Results: After meta-analysis, 19 previously unreported SNPs, including nine CB-associated and ten E-associated, had p value ≤ 0.0005. Rather than controlling for false discovery by Bonferroni adjustment, we are now assessing these SNP sets for lung tissue cis-regulatory function in the GTEx eQTL expression database and by transcript allelic-imbalance analysis in NBEC.
Conclusion: We describe a novel approach to reduce false discovery and increase power to discover COPD risk variants in existing GWAS datasets that function by affecting cis-regulation. The set of SNPs enriched for replication are confirmed to have cis-regulatory function in NBEC will be assessed for validation in an independent completed GWAS cohorts available through dbGAP