Experimental Field Statistics Validation in a Cubic Reverberation Chamber with Mechanical Mode Stirring & Bistatic Illumination

Abstract The paper gives an critical overview of measurements carried out in the cubic reverberation chamber at Royal Military Academy (2, 5 × 2, 5 × 2, 5m 3 ). The room is equiped with a mechanical mode stirrer illuminated simultaneously by two small cross-polarized ultra-wideband TEM horns. The frequencies of interest are between 800 MHz and 2,5 GHz. Though the whole validation of the chamber as immunity testing facility is still in progress, the results presented here point out that what could firstly be seen as a drawback -the cubic shape -can be effectively overcome by carefully designing the mode stirrer. Spatial homogeneity of the field, stirring efficiency ratio at different frequencies and CDF at the expected LUF (least usable frequency) are presented and critically commented

Avaliação da prevenção e controle da anemia infecciosa equina no Pantanal.

bitstream/item/80580/1/CT98.pd

Two Methods for Estimation of Amplifier Imbalances in Multi-Amplifier Transmission Structures

Energy efficient power amplification of multilevel constellations can be achieved by an amplification structure based on the constellation's as a sum of polar components, such as $M$ BPSK (Bi-Phase Shift Keying), that are separately amplified. By doing this one can define highly efficient transmitters based on multiple amplifiers. However, amplifiers' imbalances might lead to substantial constellation distortion since phase and gain imbalances cause rotations and translations of the symbols associated to each branch that are combined to generate the resulting constellation. Therefore, it becomes crucial the knowledge of the amplifiers' imbalances to overcome this problem at the receiver side. For that we propose and evaluate efficient two new methods for estimating amplifier imbalances. Simulation results demonstrate that the good performance attainable by the proposed estimate algorithms can be assured without significant increase in system and computational complexity

ELISA rgp90 - metodologia alternativa para o diagnóstico da anemia infecciosa equina no Pantanal.

Como teste oficial para diagnóstico da anemia infecciosa equina (AIE), a política governamental brasileira para prevenção e controle da AIE determina que seja usado o teste de imunodifusão em gel de ágar (IDGA) com a proteína p26 do capsídeo viral como antígeno. A Organização Mundial para a Saúde Animal (OIE) afirma que a IDGA e os testes de imunoadsorção enzimática (ELISAs) são ambos testes acurados e confiáveis, classificando os últimos como testes alternativos. Internacionalmente, há quatro ELISAs disponíveis, três usando a p26, e um a p26 e a gp45, um antígeno da transmembrana viral. Pesquisadores brasileiros desenvolveram um ELISA indireto com uma glicoproteína recombinante da superfície viral, a rgp90. Anticorpos contra a gp90 são usualmente detectados antes dos anticorpos contra a p26, e quando os níveis de replicação viral são extremamente baixos. O objetivo deste estudo preliminar foi comparar o desempenho do ELISA rgp90 com a oficialmente aceita IDGA utilizando a p26, em amostras de equídeos do Pantanal.bitstream/item/37287/1/BP93.pd

Ocorrência de Anemia Infecciosa Equina em potros de éguas soropositivas no Pantanal do Mato Grosso do Sul.

bitstream/item/80307/1/COT87.pd

Inverse Kinematics and Trajectory Planning Analysis of a Robotic Manipulator

In this work, we pretended to show and compare three methodologies used to solve the inverse kinematics of a 3 DOF robotic manipulator. The approaches are the algebraic method through Matlabreg; solve function, Genetic Algorithms (GAs), Artificial Neural Networks (ANNs). Another aspect considered is the trajectory planning of the manipulator, which allows the user to control the desired movement in the joint space. We compare polynomials of third, fourth and fifth orders for the solution of the chosen coordinates. The results show that the ANN method presented best results due to its configuration to show only feasible joint values, as also do the GA. In the trajectory planning the analysis lead to the fifth-order polynomial, which showed the smoothest solution

Phenotypic impact of deregulated expression of class I histone deacetylases in urothelial cell carcinoma of the bladder

Deregulated expression of histone deacetylases (HDACs) has been implicated in tumorigenesis. Herein, we investigated class I HDACs expression in bladder urothelial cell carcinoma (BUCC), its prognostic value and biological significance. Significantly increased transcript levels of all HDACs were found in BUCC compared to 20 normal mucosas, and these were higher in lower grade and stage tumors. Increased HDAC3 levels were associated with improved patient survival. SiRNA experiments showed decrease cell viability and motility, and increased apoptosis. We concluded that class I HDACs play an important role in bladder carcinogenesis through deregulation of proliferation, migration and apoptosis, constituting putative therapeutic targets.info:eu-repo/semantics/publishedVersio

Improved recovery of urinary small extracellular vesicles by differential ultracentrifugation

Extracellular vesicles (EVs) are lipid-membrane enclosed structures that are associated with several diseases, including those of genitourinary tract. Urine contains EVs derived from urinary tract cells. Owing to its non-invasive collection, urine represents a promising source of biomarkers for genitourinary disorders, including cancer. The most used method for urinary EVs separation is differential ultracentrifugation (UC), but current protocols lead to a significant loss of EVs hampering its efficiency. Moreover, UC protocols are labor-intensive, further limiting clinical application. Herein, we sought to optimize an UC protocol, reducing the time spent and improving small EVs (SEVs) yield. By testing different ultracentrifugation times at 200,000g to pellet SEVs, we found that 48 min and 60 min enabled increased SEVs recovery compared to 25 min. A step for pelleting large EVs (LEVs) was also evaluated and compared with filtering of the urine supernatant. We found that urine supernatant filtering resulted in a 1.7-fold increase on SEVs recovery, whereas washing steps resulted in a 0.5 fold-decrease on SEVs yield. Globally, the optimized UC protocol was shown to be more time efficient, recovering higher numbers of SEVs than Exoquick-TC (EXO). Furthermore, the optimized UC protocol preserved RNA quality and quantity, while reducing SEVs separation time.</p

Improved recovery of urinary small extracellular vesicles by differential ultracentrifugation

Extracellular vesicles (EVs) are lipid-membrane enclosed structures that are associated with several diseases, including those of genitourinary tract. Urine contains EVs derived from urinary tract cells. Owing to its non-invasive collection, urine represents a promising source of biomarkers for genitourinary disorders, including cancer. The most used method for urinary EVs separation is differential ultracentrifugation (UC), but current protocols lead to a significant loss of EVs hampering its efficiency. Moreover, UC protocols are labor-intensive, further limiting clinical application. Herein, we sought to optimize an UC protocol, reducing the time spent and improving small EVs (SEVs) yield. By testing different ultracentrifugation times at 200,000g to pellet SEVs, we found that 48 min and 60 min enabled increased SEVs recovery compared to 25 min. A step for pelleting large EVs (LEVs) was also evaluated and compared with filtering of the urine supernatant. We found that urine supernatant filtering resulted in a 1.7-fold increase on SEVs recovery, whereas washing steps resulted in a 0.5 fold-decrease on SEVs yield. Globally, the optimized UC protocol was shown to be more time efficient, recovering higher numbers of SEVs than Exoquick-TC (EXO). Furthermore, the optimized UC protocol preserved RNA quality and quantity, while reducing SEVs separation time.</p