Differential gene expression and co-regulated expression of genes in leukemia: an in-silico approach to identify potent biomarker

A biomarker can be measured, used to diagnose or classify disease, and measure progress as well as the therapeutic response of the disease. Early diagnosis and selection of appropriate treatment can be critical for the successful treatment of diseases. Identification and characterization of potent diagnostic biomarkers, and therapeutic targets rely heavily on traditional in vitro screens which require extensive resources and time. Integration of in silico screens prior to experimental validation can improve the efficiency and potency of biomarkers as well as reduce the cost and time of biomarker discovery. Considering the need, present work was undertaken to identify biomarkers for different classes of leukemia. Differential Gene Expression (DGE) analysis and co-regulated expression analysis were used for in silico identification and characterise a potent biomarker for leukemia. On the basis of in silico screening, the present study proposed seven protein-coding (CD38, TSC22D3, TNFRSF25, AGL, LARGE1, ARHGAP32, and PARM1) genes for the diagnosis of leukemia. The study also proposed a novel three-step lineage-specific model for the diagnosis of leukemia. In the three-step diagnosis model, the first group of biomarkers with an association of clinical and hematological parameters diagnose leukemia. The second group of biomarkers diagnoses acute and chronic form of leukemia. The third group of biomarkers identifies whether it belongs to myeloid lineage or lymphoid lineage

Studies on the morphology of leukaemic blast cells in relation to haematological parameters

A combination of haematological parameters with morphological evaluation of peripheral blood and bone marrow blast cells is crucial for leukaemia diagnosis. FAB (French– American–British) classification is a simple and powerful diagnostic tool for leukaemia           in developing countries like India. Differentiation block in the early stages of haematopoiesis and morphological characteristics of leukemic blast cells are directly related to haematological parameters. The present study is an approach to increase understanding of the simple morphological FAB classification of leukaemia  in relation to haematological parameters. The present study revealed that Chronic Myeloid Leukaemia  (CML) was the most common type of leukaemia , followed by Acute Myeloid Leukaemia, Acute Lymphoid Leukaemia  (ALL), and Chronic Lymphoid Leukaemia  (CLL) in Nagpur. Most of the cases of Acute Leukaemia  had severe anaemia and thrombocytopenia. Highest variation was found in Total WBCs count of different types of leukaemia , particularly in different subtypes of AML. The present study also suggested that FAB classification is not outdated, but it does require continuous revalidation and other procedures for refinement.          &nbsp

Vehicle Fuel Monitoring and Theft Detection System With SMS Indication using GSM

Today’s world want digital techniques for activity of any amount typical fuel meter area unit Analog so we have a tendency to attempting to create it digitized to indicate the fuel price digitally. In our project we have a tendency to show the quantity of fuel gift in fuel tank digitally i.e. 1lits, 1.5lits, 2lits etc. additionally fuel stealing is live drawback all told over world. In our project if fuel gets stealing then text message can send to owner of motorbike additionally buzzer makes noise so owner of motorbike get aware. In ancient vehicle system such quite system not enforced like show fuel convenience digitally & fuel stealing of motorbike are often avoided

To evaluate toxicological residues of pesticide on Brassica oleracea before and after Dhavana by Vishaghna Dravya Albizzia Lebbek

Ayurveda is classified into 8 branches Agadtantra is one of important branch of Astanga ayurved which deals with study of poisons origin their properties harmful effects fatal doses their management prevention of poisoning method of detection and estimation of poison. Poisons may be from herbs, mineral or animal origin. In modern science parallel branch known as Toxicology. Today the scope of toxicology continues to grow rapidly and the subject is to profound importance to human and animal life too. Evaluation of residues of pesticides on Cauliflower (Brassica oleracea) before and after Dhavana by Vishaghna Dravya Shirish (Albizzia Lebbek). Cauliflower (Brassica oleracea) and Shirish panchanga (Albizzia Lebbek). Identification and authentication was done in department of Dravyaguna. The percentage of Cypermethrin residue was found to be decreased more by shirish kwatha(73.86% ) than hot water(67.33) and tap water(51.56%). The percentage of Chlorpyrifos residue was found to be decreased more by shirish kwatha(48.83%) than hot water(42.93%) and tap water(35.71%). The percentage of Imidaclopride residue was found to be decreased by tap water(86.54%) than that of hot water(72.44%) and shirish kwatha (78.84%).Dhavana with shirish kwatha was found more efficient method for reduction of pesticidal residue in cauliflower