Comparison of two sampling protocols and four home-range estimators using radio-tracking data from urban badgers Meles meles

Radio-telemetry is often the method of choice for studies of species whose behaviour is difficult to observe directly. However, considerable debate has ensued about the best way of deriving home-range estimates. In recent years, kernel estimators have become the most widely used method, together with the oldest and simplest method, the minimum convex polygon (MCP). More recently, it has been suggested that the local convex hull (LCH) might be more appropriate than kernel methods in cases where an animal’s home range includes a priori inaccessible areas. Yet another method, the Brownian bridge (BB), explicitly uses autocorrelated data to determine movement paths and, ultimately, home ranges or migration routes of animals. Whereas several studies have used simulation techniques to compare these different methods, few have used data from real animals. We used radio-telemetric data from urban badgers Meles meles to compare two sampling protocols (10-minute vs at least 30-minute inter-fix intervals) and four home-range estimators (MCP, fixed kernels (FK), LCH and BB). We used a multi-response permutation procedure and randomisation tests to compare overall patterns of fixes and degree of overlap of home ranges estimated using data from different sampling protocols, and a general linear model to compare the influence of sampling protocols and home-range estimator on the size of habitat patches. The shape of the estimated home ranges was influenced by sampling protocol in some cases. By contrast, the sizes and proportions of different habitats within home ranges were influenced by estimator type but not by sampling protocol. LCH performed consistently better than FK, and is especially appropriate for patchy study areas containing frequent no-go zones. However, we recommend using LCH in combination with other methods to estimate total range size, because LCH tended to produce smaller estimates than any other method. Results relating to BB are preliminary but suggest that this method is unsuitable for species in which range size is small compared to average travel speed.Marie-Curie Intra-European Fellowship (BSSUB - 24007); Defra WSC contract WM0304; Wildlife Biology granted the permit to upload the article to this repositor

Artifacts from manganese reduction in rock samples prepared by focused ion beam (FIB) slicing for X-ray microspectroscopic analysis

Abstract. Manganese (Mn)-rich natural rock coatings, so-called rock varnishes, are discussed controversially regarding their genesis. Biogenic and abiogenic mechanisms, as well as a combination of both, have been proposed to be responsible for the Mn oxidation and deposition process. We conducted scanning transmission X-ray microscopy - near edge X-ray absorption fine structure spectroscopy (STXM-NEXAFS) measurements to examine the abundance and spatial distribution of the different oxidation states of Mn within these nano- to micrometer thick crusts. Such microanalytical measurements of thin and hard rock crusts require sample preparation with minimal contamination risk. Focused ion beam (FIB) slicing, a well-established technique in geosciences, was used in this study to obtain 100–200 nm thin slices of the samples for X-ray transmission spectroscopy. However, even though this preparation is suitable to investigate element distributions and structures in rock samples, we observed that, using standard parameters, modifications of the Mn oxidation states occur in the surfaces of the FIB slices. Based on our results, the preparation technique likely causes the reduction of Mn4+ to Mn2+/3+. We draw attention to this issue, since FIB slicing, SEM imaging, and other preparation and visualization techniques operating in the keV range are well-established in geosciences, but researchers are often unaware of the potential for reduction of Mn and possibly other elements in the samples’ surface layers

Landau Theory of Domain Wall Magnetoelectricity

We calculate the exact analytical solution to the domain wall properties in a multiferroic system with two order parameters that are coupled bi-quadratically. This is then adapted to the case of a magnetoelectric multiferroic material such as BiFeO3, with a view to examine critically whether the domain walls can account for the enhancement of magnetization reported for thin films fo this material, in view of the correlation between increasing magnetization and increasing volume fraction of domain walls as films become thinner. The present analysis can be generalized to describe a class of magnetoelectric devices based upon domain walls rather than bulk properties.Comment: 9 pages, 4 figure

Deep Hubble Space Telescope Imaging of Globular Clusters Towards the Galactic Bulge: Observations, Data Reduction and Color-Magnitude Diagrams

The Galactic globular clusters (GGCs) located towards the Galactic bulge have generally been excluded from large-scale photometric GGC surveys due to severe total and differential extinction. Here, we present an overview of a Hubble Space Telescope program designed to obtain deep, high spatial resolution multiband imaging of 16 poorly studied GGCs located towards the inner Galactic bulge and disk. In this first paper of a series resulting from these observations, we give an overview of target cluster selection, observations and data reduction procedures for optimizing the resulting photometric catalogs. Artificial star tests are used to compare the respective advantages of different data reduction strategies in terms of photometric and astrometric precision and photometric incompleteness. We present the resulting color-magnitude diagrams (CMDs) of all target clusters in several color-magnitude planes, along with CMDs of comparison fields from parallel observations. For each target cluster, we summarize existing studies, and discuss their CMDs qualitatively in the context of these results.Comment: AJ accepted. Figure quality has been reduced to meet arXiv size limit

Knowledge: its role in hunger, nutrition and climate justice

Climate change will change conditions for food and farming beyond all previous human experience. We need a new era of innovation, in which farmers and communities participate in learning networks, drawing on science and on others’ experiences to complement their local knowledge

Transplanting the leafy liverwort Herbertus hutchinsiae : A suitable conservation tool to maintain oceanic-montane liverwort-rich heath?

Thanks to the relevant landowners and managers for permission to carry out the experiments, Chris Preston for helping to obtain the liverwort distribution records and the distribution map, Gordon Rothero and Dave Horsfield for advice on choosing experimental sites and Alex Douglas for statistical advice. Juliane Geyer’s help with fieldwork was greatly appreciated. This study was made possible by a NERC PhD studentship and financial support from the Royal Botanic Garden Edinburgh and Scottish Natural Heritage.Peer reviewedPostprin

LNK suppresses interferon signaling in melanoma.

LNK (SH2B3) is a key negative regulator of JAK-STAT signaling which has been extensively studied in malignant hematopoietic diseases. We found that LNK is significantly elevated in cutaneous melanoma; this elevation is correlated with hyperactive signaling of the RAS-RAF-MEK pathway. Elevated LNK enhances cell growth and survival in adverse conditions. Forced expression of LNK inhibits signaling by interferon-STAT1 and suppresses interferon (IFN) induced cell cycle arrest and cell apoptosis. In contrast, silencing LNK expression by either shRNA or CRISPR-Cas9 potentiates the killing effect of IFN. The IFN-LNK signaling is tightly regulated by a negative feedback mechanism; melanoma cells exposed to IFN upregulate expression of LNK to prevent overactivation of this signaling pathway. Our study reveals an unappreciated function of LNK in melanoma and highlights the critical role of the IFN-STAT1-LNK signaling axis in this potentially devastating disease. LNK may be further explored as a potential therapeutic target for melanoma immunotherapy