Molecular genetic diversity of some rabbit breeds based on mitochondrial 16S rRNA sequences

[EN] The present study was performed to assess the genetic variations among six rabbit breeds in Egypt based on mitochondrial 16S rRNA sequences. The length of partial mitochondrial 16S rRNA in the six rabbit breeds ranged from 546 bp to 558 bp. The sequenced regions were submitted to GenBank/NCBI under accession numbers (MW052052 - MW052057). The average content of A+T was 57% in all breeds. Among breeds, the percentages of genetic distance values were ranged from 0.000 to 0.004. The highest P-distance (0.004) was found between the New Zealand White breed and all other breeds. The results support the suitability of mitochondrial 16S rRNA for genetic diversity analysis of rabbit breeds and the applicability for future research on genetic relationships and the phylogeny of rabbit breeds.Allam, M.; Mahrous, NS. (2021). Molecular genetic diversity of some rabbit breeds based on mitochondrial 16S rRNA sequences. World Rabbit Science. 29(3):193-201. http://hdl.handle.net/10251/173609OJS19320129

Minisum and minimax transfer point location problem with random demands points

This paper is concerned with analyzing some models of the weighted transfer point location problem under the minisum and minimax criterions when demand points are randomly distributed over regions of the plane and the location of the service facility is known. In case of minisum objective with rectilinear distance, an iterative procedure was constructed for estimating the optimal transfer point location using the hyperbolic application procedure. Exact analytic solution was obtained when the random demand points follow uniform distributions. A unified analytic optimal solution was provided for all types of probability distributions of the random demand points when the distance is the squared Euclidean distance. For minimax objective with squared Euclidean distance, an iterative procedure based on Karush-Kuhn-Tucker conditions was developed to produce an approximate solution to the optimal solution. Illustrative numerical examples were provided

Experimental research into the effect of some operation factors and rock properties on the rate of penetration

Purpose. The purpose of this research is to investigate the effect of weight on bit (WOB), rotary speed (RPM), and some rock properties on the rate of penetration (ROP). Methods. Four types of rock were collected from different sites in the south of Egypt (Aswan sandstone, Isawya limestone, Assiut limestone, and Minya marble). These tested samples were drilled by diamond core bit using a fixed laboratory diamond drilling machine under conditions (400 and 1200 rpm), and loads from 12 to 220 kg. WOB, ROP, torque (T) and specific energy (SE) were continuously observed during the drilling trials. The effect of these factors on ROP were examined. Water is used as a flushing and cooling medium. Findings. The effect of some operation factors on the penetration rate regarding mineral composition and different rock type was evaluated and the general equation was carried out to predict the suitable operation factor which gives the best penetration Rate. Originality. The effect of some operation factors on the penetration rate regarding mineral composition and different rock type was evaluated and the general equation was carried out to predict the suitable operation factor which gives the best penetration Rate. Practical implications. The Results demonstrated that ROP was inversely proportional to compressive strength (CS), tensile strength (TS), and density. It was also found that Aswan sandstone, Isawya limestone, Assiut limestone, and Minya marble have CS 111.94 MPa, 131.22 MPa, 125.0 MPa, and 502.5 MPa respectively. Was established that ROP was directly proportional to porosity. Increasing ROP in case of Aswan sandstone is associated with increase in porosity – 23.158%, and in case of Assiut limestone and Isawya limestone ROP is also associated with an increase in porosity – 19.9% and 17.93% respectively. Decreasing ROP in case of Minya marble is associated with decrease in porosity – 2.314%. So, we concluded that marble has lower ROP due to its higher CS, TS, and density and lower porosity than other tested rocks.Мета. Вивчення впливу комплексу основних експлуатаційних параметрів і ряду фізико-механічних властивостей порід на швидкість буріння в умовах різних родовищ Південного Єгипту. Методика. Були відібрані 4 типи порід з різних районів на півдні Єгипту (Асуанської піщаник, вапняк Ісав, Ассіутскій вапняк і мармур Міньї). Експериментальні зразки отримано на стаціонарній лабораторній буровій установці з алмазним долотом при швидкостях обертання 400 і 1200 об/хв та при навантаженнях від 12 до 220 кг. Під час експериментів постійно вимірювалися навантаження на долото, частота обертання, крутний момент на долоті й питома енергія, а також досліджувався вплив цих факторів на швидкість буріння. Як розчин для промивання і охолоджуючий агент використовувалася вода. Результати. Встановлено залежності швидкості буріння від навантаження на долото, питомої енергії та частоти обертання і питомої енергії. Виявлено, що для всіх чотирьох типів породи швидкість буріння збільшується з ростом навантаження на долото, в той час як питома енергія зменшується з ростом швидкості буріння. Розроблено ефективний спосіб прогнозування продуктивності бурової установки. Встановлено, що швидкість буріння обернено пропорційна міцності на стиск, міцності на розтягнення й щільності, а також змінюється прямо пропорційно пористості. Визначено, що менша швидкість буріння мармуру пов’язана з великими значеннями міцності на стиск, розтяг і щільність, а також з меншою пористістю у порівнянні з іншими досліджуваними породами. Наукова новизна. Встановлено нові закономірності взаємозв’язку експлуатаційних параметрів буріння з урахуванням різного типу породи (пісковик, вапняк, мармур) та її мінерального складу, а також обґрунтовано головну умову експлуатаційного фактору, який забезпечує оптимальну швидкість буріння. Практична значимість. Отримані результати дозволяють вибирати оптимальні параметри і режими буріння в процесі розвідки умов залягання родовищ Південного Єгипту, їх мінерального вмісту та стратиграфічних особливостей. Результати будуть корисними для проектних організацій, наукових інститутів з геології й розвідки, а також гірничих підприємств.Цель. Изучение влияния комплекса основных эксплуатационных параметров и ряда физико-механических свойств пород на скорость бурения в условиях пород различных месторождений Южного Египта. Методика. Были отобраны 4 типа пород из различных районов на юге Египта (Асуанский песчаник, известняк Исавии, Ассиутский известняк и мрамор Миньи). Экспериментальные образцы получены на стационарной лабораторной буровой установке с алмазным долотом при скоростях вращения 400 и 1200 об/мин и при нагрузках от 12 до 220 кг. Во время экспериментов постоянно измерялись нагрузки на долото, частота вращения, крутящий момент на долоте и удельная энергия, а также и исследовалось влияние этих факторов на скорость бурения. В качестве промывочного раствора и охлаждающего агента использовалась вода. Результаты. Установлены зависимости скорости бурения от нагрузки на долото, удельной энергии и частоты вращения и удельной энергии. Выявлено, что для всех четырех типов породы скорость бурения увеличивается с ростом нагрузки на долото, в то время как удельная энергия уменьшается с ростом скорости бурения. Разработан эффективный способ прогнозирования производительности буровой установки. Установлено, что скорость бурения обратно пропорциональна прочности на сжатие, прочности на растяжение и плотности, а также изменяется прямо пропорционально пористости. Определено, что меньшая скорости бурения мрамора связана с большими значениями прочности на сжатие, растяжение и плотности, а также с меньшей пористостью по сравнению с другими исследуемыми породами. Научная новизна. Получены новые закономерности взаимосвязи эксплуатационных параметров бурения с учетом различного типа породы (песчаник, известняк, мрамор) и ее минерального состава, а также обосновано главное условие эксплуатационного фактора, который обеспечивает оптимальную скорость бурения. Практическая значимость. Полученные результаты позволяют выбирать оптимальные параметры и режимы бурения в процессе разведки условий залегания месторождений Южного Египта, их минерального содержание и стратиграфических особенностей. Результаты будут полезными для проектных организаций, научных институтов по геологии и разведке, а также горных предприятий.Our sincere gratitude for Mining and Metallurgical Engineering Dept. Faculty of Engineering, Assiut, Egypt for invaluable assistance to upgrade and guidance to completed this article

Numerical investigation of laminar flow in micro-tubes with designed surface roughness

This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, Aristotle University of Thessaloniki, University of Thessaly, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute.Recently, there has been a rapid growth in applications that deal with fluid flow at micro-scale where surface roughness is a real feature in these applications. Published literature shows conflicting findings regarding the effect of surface roughness on the friction factor of laminar flow at micro-scale. The understanding of fluid flow behavior in micro-tubes is very important for effective design of micro-fluidic devices. This work presents a numerical investigation of the effect of various surface roughness geometries on friction factor in fluid flow in the laminar regime. Results indicate that surface roughness causes deviation of the frictional factor from conventional theory with various values depending on the height and shape of the roughness used

Numerical investigation of laminar flow heat transfer through helically coiled tubes using Al2O3 nanofluid

This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, Aristotle University of Thessaloniki, University of Thessaly, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute.Nanofluids have been reported to enhance heat transfer performance in heat exchangers. Additionally, the use of helical coils has shown to be another passive heat transfer enhancement technique. This work presents a CFD modeling study to investigate the laminar heat transfer through helical tubes with nanofluids. The developed CFD models were validated against published experimental results and empirical correlations in the literature. The effects of particles concentration and Reynolds number on heat transfer coefficient were numerically investigated. Results have shown that Al2O3 dispersed in water increases the heat transfer coefficient in helical coils by up to 4.5 times that of pure water in straight tubes at same Reynolds number. For concentrations larger than 2%, Al2O3 is more suitable for thermal systems of small thermal loads where the pumping power is not critical

BUILDING A COMMUNITY OF PRACTICE FOR ENGLISH-AS-A-FOREIGN LANGUAGE TUTORS DURING PRIVATE TUTORING

The creation of a community of practice of tutors--a shared practice among a group of people who share the same domain--enables second-language learners to facilitate their acquisition of English by embracing new learning strategies while they learn the target language. The community of tutors’ perspective allows for the incorporation of the individual’s particular second-language-acquisition needs and goals. This presentation presents a proposed study that took place at the Yasuda Center at California State University, San Bernardino in the summer of 2015. Students in the English Language Program housed in the College of Extended Learning were asked to participate in tutoring sessions offered by tutors who participated in a community of tutors. Tutors embraced new teaching strategies that they acquired through participating within a community of practice, sharing their background knowledge and teaching experience, and demonstrating new teaching techniques to each other by using collaborative and hybrid strategies during activities embedded in a rich learning context. The provision of community of practice for tutors in the English Language Program enabled learners to develop meaning-making and communication skills as well as language and literacy skills to address the informational and problem-solving needs of their tasks and assignments

Preparation and Characterization of Silver and Gold Nanoparticles and Study Influence on Physical Properties of PVA/PVP nanocomposites

Gold nanoparticles (Au NPs) and Silver nanoparticles (Ag NPs) ware prepared by green synthesis extraction using the Chenopodium murale leaf extract, the obtained (Ag NPs and Au NPs) were investigated by UV/Vis. absorption spectroscopy, transmission electron microscopy, Zetasizer, XRD. The plant extraction leads to produce nanoparticles of spherical shape with size range from 4 to 22 nm. Polyvinyl alcohol (PVA) and polyvinyl Pyrolidone (PVP) blend with mixed silver and gold nanoparticles were prepared by casting method. Amorphous feather of doping polymers blend was characterized by X-ray diffraction. Significant changes within the polymer matrix were monitored from infrared spectroscopy which indicates the interaction between polymer blend and mixed nanoparticles. Both indirect and direct optical energy gaps are calculated and discussed

Genetic polymorphism of five genes associated with growth traits in goat

Genetic polymorphism studies in domestic animals aim at evaluating genetic variations within and across breeds mainly for conservation purposes. In this study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect polymorphisms of five candidate genes in four Egyptian and Saudi goat breeds (Barki, Zaribi, Ardi and Masri), to detect the genotype of GH, IGF1, POUIF1, MSTN and BMP15 genes in the goat breeds and their allele frequencies. Results of GH gene which encloses a Haelll endonuclease restriction site show four unique PCR-RFLP banding patterns (genotypes AA, AB, CC and CD). The frequencies of the A allele in the samples from the goat breeds varied from 0.410 to 0.620. While  IGF-1gene revealed three fragments after digestion with Haelll with genotype AA, AB and BB and the  frequencies of allele A varied from 0.432 to 0.731. Furthermore, PCR-RFLP of POUIF1 gene showed two  fragments after digestion by Pst1 endonuclease with genotype TT and CC and the frequencies of allele T varied from 0.250 to 0.840. The MSTN gene revealed three fragments after digestion with DraI with genotype AA, BB and AB and the frequencies of allele A varied from 0.240 to 0.630. Meanwhile, the BMP15 gene revealed one fragments of 112 bp for AA after digestion with Hinf1 enzyme.Key words: Goats, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), GH, IGF-1, POUIF1, MSTN, BMP-15

CHEMICAL INVESTIGATION OF BAUHINIA VAHLII WIGHT AND ARNOTT LEAVES GROWN IN EGYPT

Objective: Plants of genus Bauhinia are famous for their rich flavonoid content. Several phytochemical and biological investigations affirmed the role of flavonoids in the different biological impacts exerted by Bauhinia plants. The present study aims to investigate the major phytoconstituents of the leaves of B. vahlii Wight and Arnott.Methods: Powdered leaves were extracted with n-hexane (HE) and the defatted marc was extracted with 70% ethanol. The defatted ethanolic extract (DEE) was further partitioned with solvents of increasing polarities. The HE and polar fractions of DEE were purified using different chromatographic techniques and isolated compounds were identified through their melting points, 1D and 2D NMR, UV and MS spectral data.Results: A total of nine compounds were isolated and identified. Taraxerol (1), a pentacyclic triterpene, and β-sitosterol (2) were isolated from HE. Investigation of polar fractions of DEE yielded six flavonoids and a phenolic acid, namely luteolin (3), quercetin (4), gallic acid (5), avicularin (6), quercitrin (7), hyperoside (8) and quercetin-3-O-β-sophoroside (9).Conclusion: Flavonols of the quercetin nucleus were the major detected constituents in B. vahlii leaves. Taraxerol, avicularin and quercetin-3-O-β-sophoroside are isolated for the first time from the genus Bauhinia. Results of this study encourage future pharmacological investigation of B. vahlii due to the presence of biologically active flavonoids and phytosterols.Keywords: Bauhinia vahlii Wight, Arnott., Polar extractives, Flavonols, Quercetin, TaraxerolÂ