How to account for irrigation withdrawals in a watershed model

In agricultural areas, the downstream flow can be highly influenced by human activities during low-flow periods, especially during dam releases and irrigation withdrawals. Irrigation is indeed the major use of freshwater in the world. This study aims at precisely taking these factors into account in a watershed model. The Soil and Water Assessment Tool (SWAT+) agro-hydrological model was chosen for its capacity to model crop dynamics and management. Two different crop models were compared in terms of their ability to estimate water needs and actual irrigation. The first crop model is based on air temperature as the main determining factor for growth, whereas the second relies on high-resolution data from the Sentinel-2 satellite to monitor plant growth. Both are applied at the plot scale in a watershed of 800 km2 that is characterized by irrigation withdrawals. Results show that including remote sensing data leads to more realistic modeled emergence dates for summer crops. However, both approaches have proven to be able to reproduce the evolution of daily irrigation withdrawals throughout the year. As a result, both approaches allowed us to simulate the downstream flow with a good daily accuracy, especially during low-flow periods.</p

Particle-induced morphological modification of Al alloy equiaxed dendrites revealed by sub-second in situ microtomography

The study of dendritic growth is a challenging topic at the heart of intense research in material science. Understanding such processes is of prime importance as it helps predicting the final microstructure governing material properties. In the specific case of the design of metal-matrix nanocomposites (MMNCs), the addition of nano-sized particles inside the metallic melt increases the complexity as their influence on the growth morphology of dendrites is not yet fully understood. In the present experimental study, we use in situ X-ray tomography imaging with very high temporal resolution (0.35 s per 3D image) coupled with in situ ultrasonic melt homogenisation to record, in 3D and real time, the free growth at high cooling rates (~2 K.s-1) of equiaxed dendrites in an AA6082 alloy containing Y2O3 nanoparticles. The careful 3D analysis of the dendrite morphologies as well as their solidification dynamics reveals that in the case of well-dispersed particles, dendrite equiaxed growth occurs through complex hyper-branched morphologies. Such behaviour is believed to arise from particle-induced modification of the solidification processes at the origin of multiple splitting, branching and curving mechanisms of the dendrite arms. These results shed light on long-standing empirical and modelling statements and open new ways for direct investigation of equiaxed growth in metallic alloys and composites.European Commission in the 7th Framework Program (contract FP7-NMP3-LA-2012-280421) ExoMet Project by the European Space Agency and by the individual partner organizations; ESRF-MA1876 long term project

A Model for the Development of the Rhizobial and Arbuscular Mycorrhizal Symbioses in Legumes and Its Use to Understand the Roles of Ethylene in the Establishment of these two Symbioses

We propose a model depicting the development of nodulation and arbuscular mycorrhizae. Both processes are dissected into many steps, using Pisum sativum L. nodulation mutants as a guideline. For nodulation, we distinguish two main developmental programs, one epidermal and one cortical. Whereas Nod factors alone affect the cortical program, bacteria are required to trigger the epidermal events. We propose that the two programs of the rhizobial symbiosis evolved separately and that, over time, they came to function together. The distinction between these two programs does not exist for arbuscular mycorrhizae development despite events occurring in both root tissues. Mutations that affect both symbioses are restricted to the epidermal program. We propose here sites of action and potential roles for ethylene during the formation of the two symbioses with a specific hypothesis for nodule organogenesis. Assuming the epidermis does not make ethylene, the microsymbionts probably first encounter a regulatory level of ethylene at the epidermis–outermost cortical cell layer interface. Depending on the hormone concentrations there, infection will either progress or be blocked. In the former case, ethylene affects the cortex cytoskeleton, allowing reorganization that facilitates infection; in the latter case, ethylene acts on several enzymes that interfere with infection thread growth, causing it to abort. Throughout this review, the difficulty of generalizing the roles of ethylene is emphasized and numerous examples are given to demonstrate the diversity that exists in plants

3-Deazaneplanocin A (DZNep), an Inhibitor of the Histone Methyltransferase EZH2, Induces Apoptosis and Reduces Cell Migration in Chondrosarcoma Cells

ObjectiveGrowing evidences indicate that the histone methyltransferase EZH2 (enhancer of zeste homolog 2) may be an appropriate therapeutic target in some tumors. Indeed, a high expression of EZH2 is correlated with poor prognosis and metastasis in many cancers. In addition, 3-Deazaneplanocin A (DZNep), an S-adenosyl-L homocysteine hydrolase inhibitor which induces EZH2 protein depletion, leads to cell death in several cancers and tumors. The aim of this study was to determine whether an epigenetic therapy targeting EZH2 with DZNep may be also efficient to treat chondrosarcomas.MethodsEZH2 expression was determined by immunohistochemistry and western-blot. Chondrosarcoma cell line CH2879 was cultured in the presence of DZNep, and its growth and survival were evaluated by counting adherent cells periodically. Apoptosis was assayed by cell cycle analysis, Apo2.7 expression using flow cytometry, and by PARP cleavage using western-blot. Cell migration was assessed by wound healing assay.ResultsChondrosarcomas (at least with high grade) highly express EZH2, at contrary to enchondromas or chondrocytes. In vitro, DZNep inhibits EZH2 protein expression, and subsequently reduces the trimethylation of lysine 27 on histone H3 (H3K27me3). Interestingly, DZNep induces cell death of chondrosarcoma cell lines by apoptosis, while it slightly reduces growth of normal chondrocytes. In addition, DZNep reduces cell migration.ConclusionThese results indicate that an epigenetic therapy that pharmacologically targets EZH2 via DZNep may constitute a novel approach to treat chondrosarcomas

Genome-Wide Crossover Distribution in Arabidopsis thaliana Meiosis Reveals Sex-Specific Patterns along Chromosomes

In most species, crossovers (COs) are essential for the accurate segregation of homologous chromosomes at the first meiotic division. Their number and location are tightly regulated. Here, we report a detailed, genome-wide characterization of the rate and localization of COs in Arabidopsis thaliana, in male and female meiosis. We observed dramatic differences between male and female meiosis which included: (i) genetic map length; 575 cM versus 332 cM respectively; (ii) CO distribution patterns: male CO rates were very high at both ends of each chromosome, whereas female CO rates were very low; (iii) correlations between CO rates and various chromosome features: female CO rates correlated strongly and negatively with GC content and gene density but positively with transposable elements (TEs) density, whereas male CO rates correlated positively with the CpG ratio. However, except for CpG, the correlations could be explained by the unequal repartition of these sequences along the Arabidopsis chromosome. For both male and female meiosis, the number of COs per chromosome correlates with chromosome size expressed either in base pairs or as synaptonemal complex length. Finally, we show that interference modulates the CO distribution both in male and female meiosis