Comparison of the effect of different resistance training frequencies on phase angle and handgrip strength in obese women: A randomized controlled trial

Phase angle (PA) is a strong predictor of sarcopenia, fragility, and risk of mortality in obese people, while an optimal muscular function and handgrip strength (HS) are required to perform different daily activities. Although there is a general agreement that resistance training improves health status in obese people, the optimal weekly training frequency forPA and physical performance parameters is not clear.This study aimed to compare the effects of different weekly resistance training frequencies performed over a 24week exercise program on PA and HSin obese people. Forty-two women (56.2 \ub1 9.1 years, body mass index (BMI) 37.1 \ub1 4.9 kg/m2) were randomly allocatedto one of two groups: a group with a highweekly training frequency of three times a week (HIGH, n= 21) and a group that performed only one weekly session (LOW, n= 21). The groups trained with an identical exercise intensity and volume per session for 6 months. Before and after the intervention period, the participants were assessed for anthropometric measures, bioimpedance analysis, and HS. There was a significant group 7 time interaction (p<0.05) for waist circumference, bioimpedance reactance divided by body height (Xc/H), PA, and HS measures. In addition, only the HIGH group increased Xc/H, PA, and HS after the intervention period (p<0.05), even after adjusting for weight loss and menopausal status. Physical exercise performed three times a week promotes better adaptations in PA and HSwhen compared with the same program performed once a week in obese women

Gellan hydrogel as a powerful tool in paper cleaning process: a detailed study

Hypothesis: Wet cleaning of ancient papers is one of the most critical steps during a conservation treatment. It is used to improve the optical qualities of a graphic work and remove dust and by-products resulting from cellulose degradation. Nevertheless, washing treatment usually involves a substantial impact on the original morphological structure of paper and can sometimes be dangerous for water sensitive inks and pigments. Experiments: The use of rigid hydrogel of Gellan gum as an alternative paper cleaning treatment is developed. The application of a rigid hydrogel minimizes damages caused by the use of water, and therefore is much more respectful for the original integrity of ancient paper. Findings: Gellan hydrogel has been used to clean paper samples belonging to different centuries (from XVI to XIX) and therefore, characterized by a different story in terms of degradation condition and paper composition. Several techniques, such as high-performance liquid chromatography, Fourier transform infrared spectroscopy, scanning electron microscopy and pH measurements, has been employed to assess the effectiveness and safety of the proposed cleaning method

Gender is conceptualized in different ways across cultures

Gender can be considered an embodied social concept encompassing biological and cultural components. In this study, we explored whether the concept of gender varies as a function of different cultural and linguistic norms by comparing communities that vary in their social treatment of gender-related issues and linguistic encoding of gender. In Study 1, Italian, Dutch, and English-speaking participants completed a free-listing task, which showed Italians and Dutch were the most distinct in their conceptualization of gender: Italian participants focused more on socio-cultural features (e.g., discrimination, politics, and power), whereas Dutch participants focused more on the corporeal sphere (e.g., hormones, breasts, and genitals). Study 2 replicated this finding focusing on Italian and Dutch and using a typicality rating task: socio-cultural and abstract features were considered as more typical of "gender"by Italian than Dutch participants. Study 3 addressed Italian and Dutch participants' explicit beliefs about gender with a questionnaire measuring essentialism and constructivism, and consolidated results from Studies 1 and 2 showing that Dutch participants endorsed more essentialist beliefs about gender than Italian participants. Consistent with socio-cultural constructivist accounts, our results provide evidence that gender is conceptualized differently by diverse groups and is adapted to specific cultural and linguistic environments

A new sustainable and innovative work for paper artworks cleaning process: Gellan hydrogel combined with hydrolytic enzymes

Paper has been used as writing and drawing support for thousands of years. The conservation of paper artworks plays a fundamental role in the field of our cultural heritage. Moreover, restoration of paper artworks is difficult due to their inherent fragility, the presence of many components and their degradation state. Among the factors that may contribute to paper deterioration are the use of glue for the application of different materials (as a lining, mounting or as a repair intervention) on the paper artifact. During a natural ageing process, glue become yellow, acid and less compact, accelerating the degradation processes of the artwork itself. The removal of glues from paper artworks represents, therefore, an important procedure for their preservation. Here we present a sustainable alternative to the common removal systems (e.g. solvents or localized enzymatic packs on the support to be cleaned). For this goal we used a rigid Gellan hydrogel (totally removable in one step) containing hydrolytic enzyme, such as proteinase K. The enzyme works as a selective cleaning agent hydrolyzing animal glues into smaller fragments, soluble into the gel. Our system represents an effective alternative to the traditional techniques because it is easy to be prepared, eco-friendly and efficient

Trichogin GA IV Alignment and Oligomerization in Phospholipid Bilayers

The membrane interactions and structure of the natural antimicrobial peptide trichogin GA IV have been investigated by CD, ATR FTIR and solid\u2010state NMR spectroscopy. A model emerges in which the peptide preferentially aligns parallel to the membrane surface and forms dimeric and tetrameric assemblies at higher concentrations

Ultrasound-Stimulated PVA Microbubbles for Adhesive Removal from Cellulose-Based Materials: A Groundbreaking Low-Impact Methodology

In this work, we shed new light on ultrasound contrast agents applied to the field of cultural heritage as an invaluable fine-tune cleaning tool for paper artworks. In this context, one of the primary and challenging issues is the removal of modern adhesives from paper artifacts. Modern adhesives are synthetic polymers whose presence enhances paper degradation and worsens its optical features. A thorough analytical and high-spatial-resolution combined study was successfully performed to test the capability of poly(vinyl alcohol)-based microbubbles stimulated by a proper noninvasive 1 MHz ultrasound field exposure in removing these adhesives from paper surfaces, in the absence of volatile invasive and toxic chemicals and without damaging paper and/or leaving residues. We demonstrate that poly(vinyl alcohol)-shelled microbubbles are suitable for interacting with paper surfaces, targeting and boosting in a few minutes the nondamaging removal of adhesive particles from paper samples thanks to their peculiar shell composition together with their ultrasound dynamics

Acclimation to different depths by the marine angiosperm Posidonia oceanica: transcriptomic and proteomic profiles

For seagrasses, seasonal and daily variations in light and temperature represent the mains factors driving their distribution along the bathymetric cline. Changes in these environmental factors, due to climatic and anthropogenic effects, can compromise their survival. In a framework of conservation and restoration, it becomes crucial to improve our knowledge about the physiological plasticity of seagrass species along environmental gradients. Here, we aimed to identify differences in transcriptomic and proteomic profiles, involved in the acclimation along the depth gradient in the seagrass Posidonia oceanica, and to improve the available molecular resources in this species, which is an important requisite for the application of eco-genomic approaches. To do that, from plant growing in shallow (−5 m) and deep (−25 m) portions of a single meadow, (i) we generated two reciprocal Expressed Sequences Tags (EST) libraries using a Suppressive Subtractive Hybridization (SSH) approach, to obtain depth/specific transcriptional profiles, and (ii) we identified proteins differentially expressed, using the highly innovative USIS mass spectrometry methodology, coupled with 1D-SDS electrophoresis and labeling free approach. Mass spectra were searched in the open source Global Proteome Machine (GPM) engine against plant databases and with the X!Tandem algorithm against a local database. Transcriptional analysis showed both quantitative and qualitative differences between depths. EST libraries had only the 3% of transcripts in common. A total of 315 peptides belonging to 64 proteins were identified by mass spectrometry. ATP synthase subunits were among the most abundant proteins in both conditions. Both approaches identified genes and proteins in pathways related to energy metabolism, transport and genetic information processing, that appear to be the most involved in depth acclimation in P. oceanica. Their putative rules in acclimation to depth were discussed

The NICMOS Snapshot Survey of nearby Galaxies

We present ``snapshot'' observations with the NearInfrared Camera and MultiObject Spectrometer (NICMOS) on board the Hubble Space Telescope (HST) of 94 nearby galaxies from the Revised Shapley Ames Catalog. Images with 0.2 as resolution were obtained in two filters, a broad-band continuum filter (F160W, roughly equivalent to the H-band) and a narrow band filter centered on the Paschen alpha line (F187N or F190N, depending on the galaxy redshift) with the 51x51 as field of view of the NICMOS camera 3. A first-order continuum subtraction is performed, and the resulting line maps and integrated Paschen alpha line fluxes are presented. A statistical analysis indicates that the average Paschen alpha surface brightness {\bf in the central regions} is highest in early-type (Sa-Sb) spirals.Comment: Original contained error in flux calibration. Table 1 now has correct Paschen Alpha fluxes. 14 pages LaTeX with JPEG and PS figures. Also available at http://icarus.stsci.edu/~boeker/publications.htm

Erratum:corrigendum: transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

Scientific Data 3:160115 doi: 10.1038/sdata.2016.115 (2016); Published 20 December 2016; Updated 14 March 2017 The original version of this Data Descriptor contained a typographical error in the spelling of the author A. Lamontanara, which was incorrectly given as A. Lamontara. This has now been corrected in the PDF and HTML versions of the Data Descriptor.</jats:p

The modulation of leaf metabolism plays a role in salt tolerance of Cymodocea nodosa exposed to hypersaline stress in mesocosms

Applying proteomics, we tested the physiological responses of the euryhaline seagrass Cymodocea nodosa to deliberate manipulation of salinity in a mesocosm system. Plants were subjected to a chronic hypersaline condition (43 psu) to compare protein expression and plant photochemistry responses after 15 and 30 days of exposure with those of plants cultured under normal/ambient saline conditions (37 psu). Results showed a general decline in the expression level of leaf proteins in hypersaline stressed plants, with more intense reductions after long-lasting exposure. Specifically, the carbon-fixing enzyme RuBisCo displayed a lower accumulation level in stressed plants relative to controls. In contrast, the key enzymes involved in the regulation of glycolysis, cytosolic glyceraldehyde-3-phosphate dehydrogenase, enolase 2 and triose-phosphate isomerase, showed significantly higher accumulation levels. These responses suggested a shift in carbon metabolism in stressed plants. Hypersaline stress also induced a significant alteration of the photosynthetic physiology of C. nodosa by means of a downregulation in structural proteins and enzymes of both PSII and PSI. However we found an over-expression of the cytochrome b559 alpha subunit of the PSII initial complex, which is a receptor for the PSII core proteins involved in biogenesis or repair processes and therefore potentially involved in the absence of effects at the photochemical level of stressed plants. As expected hypersalinity also affects vacuolar metabolism by increasing the leaf cell turgor pressure and enhancing the up-take of Na+ by overaccumulating the tonoplast specific intrinsic protein pyrophosphate-energized inorganic pyrophosphatase (H(+)-PPase) coupled to the Na+/H+-antiporter. The modulation of carbon metabolism and the enhancement of vacuole capacity in Na+ sequestration and osmolarity changes are discussed in relation to salt tolerance of C. nodosa.Postprin