ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF UREA/ THIOUREA DERIVATIVES OF 5-METHYL-3-(UREDIOMETHYL)-HEXANOIC ACID

A series of urea/ thiourea derivatives of 5-methyl-3-(urediomethyl)-hexanoic acid has been successfully synthesized from the reaction of 3-aminomethyl-5-methylhexanoic acid and aryl isocyanate/ aryl isothiocyanates in presence of triethylamine base in tetrahydrofuran solvent at rt-40C by stirring the contents for 3h

Animal model integration to AutDB, a genetic database for autism

<p>Abstract</p> <p>Background</p> <p>In the post-genomic era, multi-faceted research on complex disorders such as autism has generated diverse types of molecular information related to its pathogenesis. The rapid accumulation of putative candidate genes/loci for Autism Spectrum Disorders (ASD) and ASD-related animal models poses a major challenge for systematic analysis of their content. We previously created the Autism Database (AutDB) to provide a publicly available web portal for ongoing collection, manual annotation, and visualization of genes linked to ASD. Here, we describe the design, development, and integration of a new module within AutDB for ongoing collection and comprehensive cataloguing of ASD-related animal models.</p> <p>Description</p> <p>As with the original AutDB, all data is extracted from published, peer-reviewed scientific literature. Animal models are annotated with a new standardized vocabulary of phenotypic terms developed by our researchers which is designed to reflect the diverse clinical manifestations of ASD. The new Animal Model module is seamlessly integrated to AutDB for dissemination of diverse information related to ASD. Animal model entries within the new module are linked to corresponding candidate genes in the original "Human Gene" module of the resource, thereby allowing for cross-modal navigation between gene models and human gene studies. Although the current release of the Animal Model module is restricted to mouse models, it was designed with an expandable framework which can easily incorporate additional species and non-genetic etiological models of autism in the future.</p> <p>Conclusions</p> <p>Importantly, this modular ASD database provides a platform from which data mining, bioinformatics, and/or computational biology strategies may be adopted to develop predictive disease models that may offer further insights into the molecular underpinnings of this disorder. It also serves as a general model for disease-driven databases curating phenotypic characteristics of corresponding animal models.</p

Electrocatalytic activity of Cu2O nanocubes-based electrode for glucose oxidation

A direct electrocatalytic activity of glucose oxidation on cuprous oxide modified glassy carbon electrode is reported. Cu2O nanocubes were synthesized by a simple wet chemical route in the absence of surfactants. Purity, shape and morphology of Cu2O are characterized by XRD, SEM, XPS and DRS-UV. The Cu2O nanocubes-modified glassy carbon electrode (GCE) exhibited high electrocatalytic activity towards glucose oxidation compared with bare GCE electrode. At an applied potential of +0.60 V, the Cu2O electrode presented a high sensitivity of 121.7 mu A/mM. A linear response was obtained from 0 to 500 mu M, a response time less than 5 s and a detection limit of 38 mu M (signal/noise=3). The Cu2O nanocubes-modified electrode was stable towards interfering molecules like uric acid (UA), ascorbic acid (AA) and dopamine (DA). In short, a facile chemical preparation process of cuprous oxide nanocubes, and the fabricated modified electrode allow highly sensitive, selective, and fast amperometric sensing of glucose, which is promising for the future development of non-enzymatic glucose sensors

Comparison of dexmedetomidine and ketamine versus propofol and ketamine for procedural sedation in children undergoing minor cardiac procedures in cardiac catheterization laboratory

Background: The ideal anaesthetic technique for management of paediatric patients scheduled to undergo cardiac catheterisation is still not standardised. Aim: To compare the effects of ketamine-propofol and ketamine-dexmedetomidine combinations on hemodynamic parameters and recovery time in paediatric patients undergoing minor procedures and cardiac catheterisation under sedation for various congenital heart diseases. Material and Methods: 60 children of either sex undergoing cardiac catheterisation were randomly assigned into two groups Dexmedetomidine-ketamine group (DK) and Propofol-ketamine (PK) of 30 patients each. All patients were premedicated with glycopyrrolate and midazolam (0.05mg/kg) intravenously 5-10 min before anaesthetic induction. Group 'DK'received dexmedetomidineiv infusion 1 μg/kg over 10 min + ketamine1mg/kg bolus, followed by iv infusion of dexmedetomidine 0.5μg/kg/hr and of ketamine1 mg/kg/hr. Group 'PK' received propofol 1mg/kg and ketamine 1mg/kg/hr for induction followed by iv infusion of propofol 100 μg/kg/hr and ketamine 1 mg/kg/hr for maintenance. Haemodynamic parameters and recovery time was recorded postoperatively. Statistical Analysis: Independent sample t test was used to compare the statistical significance of continuous variables of both the groups.Chi square test was used for numerical data like gender.Fischer exact test was applied for non parametric data like ketamine consumption. Results: We observed that heart rate in dexmedetomidine (DK) group was significantly lower during the initial 25 mins after induction compared to the propofol (PK) group. Recovery was prolonged in the DK group compared to the PK group (40.88 vs. 22.28 min). Even ketamine boluses consumption was higher in DK group. Conclusion: Use of dexmedetomidine-ketamine combination is a safe alternative, without any hemodynamic orrespiratory effects during the cardiac catheterization procedure but with some delayed recovery

The Spindle Assembly Checkpoint Safeguards Genomic Integrity of Skeletal Muscle Satellite Cells

Summary To ensure accurate genomic segregation, cells evolved the spindle assembly checkpoint (SAC), whose role in adult stem cells remains unknown. Inducible perturbation of a SAC kinase, Mps1, and its downstream effector, Mad2, in skeletal muscle stem cells shows the SAC to be critical for normal muscle growth, repair, and self-renewal of the stem cell pool. SAC-deficient muscle stem cells arrest in G1 phase of the cell cycle with elevated aneuploidy, resisting differentiation even under inductive conditions. p21CIP1 is responsible for these SAC-deficient phenotypes. Despite aneuploidy’s correlation with aging, we find that aged proliferating muscle stem cells display robust SAC activity without elevated aneuploidy. Thus, muscle stem cells have a two-step mechanism to safeguard their genomic integrity. The SAC prevents chromosome missegregation and, if it fails, p21CIP1-dependent G1 arrest limits cellular propagation and tissue integration. These mechanisms ensure that muscle stem cells with compromised genomes do not contribute to tissue homeostasis

The Spindle Assembly Checkpoint Safeguards Genomic Integrity of Skeletal Muscle Satellite Cells

© 2015 The Authors. To ensure accurate genomic segregation, cells evolved the spindle assembly checkpoint (SAC), whose role in adult stem cells remains unknown. Inducible perturbation of a SAC kinase, Mps1, and its downstream effector, Mad2, in skeletal m

The assessment of P-wave dispersion and myocardial repolarization parameters in patients with chronic kidney disease

PubMed ID: 29285964OBJECTIVE: The risks of sudden death and cardiac arrhythmia are increased in patients with chronic kidney disease (CKD). Here, we aimed to evaluate the indicators of arrhythmias, such as p-wave dispersion (P-WD), QTc dispersion, Tp-e and Tp-e/QT ratio in patients with CKD stages 3-5 on no renal replacement therapy (RRT).MATERIAL AND METHODS: One-hundred and thirty three patients with CKD stages 3-5 and 32 healthy controls were enrolled into the study. No patients received RRT. QTc dispersion, P-WD and Tp-e interval were measured using electrocardiogram and Tp-e/QT ratio was also calculated.RESULTS: Mean age rates were found similar in patients and controls (60.8?±?14.2 and 61?±?12.9?y, p?=?.937, respectively). Compared patients with controls, P-WD (45.85?±?12.42 vs. 21.17?±?6.6?msec, p?<?.001), QTc-min (366.99?±?42.31 vs. 387.15?±?20.5?msec, p?<?.001), QTc dispersion (71.13?±?27.95 vs. 41.25?±?14.55?msec, p?<?.001), Tp-e maximum (81.04?±?10.34 vs. 75.49?±?10.9?msec, p?<?.001), Tp-e minimum (62.25?±?7.58 vs. 54.8?±?6.72?msec, p?<?.001) and Tp-e/QTc ratio (0.19?±?0.02 vs. 0.18?±?0.01, p?=?.001) were found to be different. QTc-max and Tp-e interval were found to be similar in both groups.CONCLUSION: P-WD and QTc dispersion, Tp-e interval and Tp-e/QTc ratio were found to be increased in with CKD stages 3-5 on no RRT

Highly dispersed Cu(II), Co(II) and Ni(II) catalysts covalently immobilized on imine-modified silica for cyclohexane oxidation with hydrogen peroxide

This paper describes the synthesis of Cu(II), Co(II) and Ni(II) catalysts immobilized on imine-functionalized silica gel through a 3-aminopropyltriethoxysilane linker. The synthesized catalysts were characterized by spectroscopic techniques, namely EDS, FTIR, UV-Vis, Si-29 MAS NMR, powder XRD and ESR spectroscopy. These analytical methods evidently confirmed the formation of silica-supported catalysts. Thermal properties of catalysts were studied between 30 and 800 degrees C by thermogravimetric-differential thermal gravimetric (TG-DTG) analysis. The surface roughness of the silica gel was increased upon modification but without losing its lumpy shape, as evidenced by SEM investigation. Magnified SEM and AFM images both suggested the high dispersive nature of the catalysts. Cyclohexane was successfully converted into cyclohexanol and cyclohexanone by the catalysts with the aid of hydrogen peroxide (oxidant). Comparatively, Cu(II) catalyst exhibited better cyclohexane conversion than the other two catalysts. The reusable nature of the catalysts was established by performing five consecutive catalytic runs with Cu(II) catalyst. Comparatively, the present reported catalytic systems were simple, reusable and effective models for higher cyclohexane conversion with better product selectivity