30 research outputs found
Phylloseptin-1 is leishmanicidal for amastigotes of Leishmania amazonensis inside infected macrophages
Leishmania protozoans are the causal agents of neglected diseases that represent an
important public health issue worldwide. The growing occurrence of drug-resistant strains of
Leishmania and severe side effects of available treatments represent an important challenge for the
leishmaniases treatment. We have previously reported the leishmanicidal activity of phylloseptin-1
(PSN-1), a peptide found in the skin secretion of Phyllomedusa azurea (=Pithecopus azureus),
against Leishmania amazonensis promastigotes. However, its impact on the amastigote form of
L. amazonensis and its impact on infected macrophages are unknown. In this work, we evaluated the
effects of PSN-1 on amastigotes of L. amazonensis inside macrophages infected in vitro. We assessed
the production of hydrogen peroxide and nitric oxide, as well as the levels of inflammatory and
immunomodulatory markers (TGF-β, TNF-α and IL-12), in infected and non-infected macrophages
treated with PSN-1. Treatment with PSN-1 decreased the number of infected cells and the number
of ingested amastigotes per cell when compared with the untreated cells. At 32 µM (64 µg/mL),
PSN-1 reduced hydrogen peroxide levels in both infected and uninfected macrophages, whereas it
had little effect on NO production or TGF-β release. The effect of PSN-1 on IL-12 and TNF-α secretion
depended on its concentration, but, in general, their levels tended to increase as PSN-1 concentration
increased. Further in vitro and in vivo studies are needed to clarify the mechanisms of action of
PSN-1 and its interaction with the immune system aiming to develop pharmacological applications
In silico, in vitro and in vivo toxicological assessment of BPP-BrachyNH2, A vasoactive proline-rich oligopeptide from Brachycephalus ephippium.
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In Silico, In Vitro and In Vivo Toxicological Assessment of BPP-BrachyNH2, A Vasoactive Proline-Rich Oligopeptide from Brachycephalus ephippium
BPP-BrachyNH2 is a proline-rich oligopeptide (PRO) firstly identified in skin secretion of the frog Brachycephalus ephippium, which possess in vitro inhibitory activity of angiotensin-I converting enzyme (ACE) and endothelium-dependent vasorelaxant activity. Considering its potential application in the treatment of cardiovascular diseases, the present work assessed the toxicological profile of the BPP-BrachyNH2. The in silico toxicity prediction was performed from the best model obtained through the optimization of the FASTA query peptide. This prediction study revealed that BPP-BrachyNH2 induced high predicted LD50 values for both humans and rats, and then is well-tolerated in the recommended range. The MTT assay was applied for the in vitro cytotoxic evaluation in murine macrophages. In this assay, a decrease of cell viability was not observed. The in vivo acute toxicological study was performed after the intraperitoneal administration of BPP-BrachyNH2 at doses of 5 and 50 mg/kg. After intraperitoneal administration, no death, alterations in behavioral parameters or weight gain curve was observed, as well as none in the serum biochemical parameters, and gross pathological and histopathological analyses. These observations demonstrates an acceptable safety profile for BPP-BrachyNH2, leading towards further studies focused on investigation of pharmacological and therapeutical applications for this peptide.info:eu-repo/semantics/publishedVersio