Determination of cell proliferation in dexamethasone-exposed human lens epithelial cells

Quantitative determination of cell proliferation showed an increase in the number of Ki-67 positive cells in human lens epithelial cells exposed to 0.1 µM dexamethasone for 24 h whereas higher concentrations of dexamethasone did not significantly affect cell proliferation. At least 300 cells in three different fields were counted. The mean and SEM were calculated from three separate chamber slides (n=3). The experiment was repeated twice with different cell lines, obtaining similar results.<p><b>Copyright information:</b></p><p>Taken from "Effects of dexamethasone on human lens epithelial cells in culture"</p><p></p><p>Molecular Vision 2008;14():1344-1352.</p><p>Published online 21 Jul 2008</p><p>PMCID:PMC2480483.</p><p></p

Long-Term Outcomes of the Minimally Invasive Ponto Surgery vs. Linear Incision Technique With Soft Tissue Preservation for Installation of Percutaneous Bone Conduction Devices

Objective: Comparing the surgical outcomes of the Minimally Invasive Ponto Surgery (MIPS) technique with the linear incision technique with soft tissue preservation (LITT-P) for bone conduction devices after a follow-up of 22 months.Methods: In this multicenter randomized controlled trial, there was the inclusion of 64 adult patients eligible for unilateral surgery. There was 1:1 randomization to the MIPS (test) or the LITT-P (control) group. The primary outcome was an (adverse) soft tissue reaction. Secondary outcomes were pain, loss of sensibility, soft tissue height/overgrowth, skin sagging, implant loss, Implant Stability Quotient measurements, cosmetic scores, and quality of life questionnaires.Results: Sixty-three subjects were analyzed in the intention-to-treat population. No differences were found in the presence of (adverse) soft tissue reactions during complete follow-up. Also, there were no differences in pain, wound dehiscence, skin level, soft tissue overgrowth, and overall quality of life. Loss of sensibility (until 3-month post-surgery), cosmetic scores, and skin sagging outcomes were better in the MIPS group. The Implant Stability Quotient was higher after the LITT-P for different abutment lengths at various points of follow-up. Implant extrusion was nonsignificantly higher after the MIPS (15.2%) compared with LITT-P (3.3%).Conclusion: The long-term results show favorable outcomes for both techniques. The MIPS is a promising technique with some benefits over the LITT-P.Concerns regarding nonsignificantly higher implant loss may be overcome with future developments and research

Short-term results from seventy-six patients receiving a bone-anchored hearing implant installed with a novel minimally invasive surgery technique

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