Chloroplast SSR markers to assess DNA diversity in wild and cultivated grapevines

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Evidence for a Sympatric Origin of Ribolla gialla, Gouais Blanc and Schiava cultivars (V. vinifera L.)

Ribolla gialla is an autochthonous grape variety cultivated in Friuli Venezia Giulia (Italy) and in Slovenia, and probably originated in Eastern Central Europe. Just like Ribolla gialla, the Gouais blanc and Schiava cultivars also appear to have originated in the same geographical area. To verify this hypothesis, a pool of varieties probably sharing the same historical and geographical origin were taken into account. The fingerprinting, by 35 SSR loci, of Gouais blanc, Ribolla gialla, Schiava grossa and Schiava lombarda (synonyms of Schiava bresciana), is presented and the correlation among historical, geographical and genetic information of these cultivars was investigated. Gouais blanc and Traminer, already suggested as key varieties in the development of European grape diversity and as parents for some French varieties, interestingly enough seems to be linked to Ribolla gialla. The putative parentage was verified using 58 microsatellite markers. The genetic results suggest a common geographical origin for Gouais blanc, Ribolla gialla and the Schiava group: these cultivars appear to be related through sympatric origin. The hypothesis of Ribolla gialla as a progeny of Gouais blanc and Traminer was ruled out. The data proved a second-degree relationship between Gouais blanc and Ribolla gialla and a third or more distant degree of relationships between Ribolla gialla and Traminer

Evidence for a Sympatric Origin of Ribolla gialla, Gouais Blanc and Schiava cultivars (V. vinifera L.)

Ribolla gialla is an autochthonous grape variety cultivated in Friuli Venezia Giulia (Italy) and in Slovenia,and probably originated in Eastern Central Europe. Just like Ribolla gialla, the Gouais blanc and Schiavacultivars also appear to have originated in the same geographical area. To verify this hypothesis, a poolof varieties probably sharing the same historical and geographical origin were taken into account. Thefingerprinting, by 35 SSR loci, of Gouais blanc, Ribolla gialla, Schiava grossa and Schiava lombarda(synonyms of Schiava bresciana), is presented and the correlation among historical, geographical andgenetic information of these cultivars was investigated. Gouais blanc and Traminer, already suggested askey varieties in the development of European grape diversity and as parents for some French varieties,interestingly enough seems to be linked to Ribolla gialla. The putative parentage was verified using 58microsatellite markers. The genetic results suggest a common geographical origin for Gouais blanc,Ribolla gialla and the Schiava group: these cultivars appear to be related through sympatric origin. Thehypothesis of Ribolla gialla as a progeny of Gouais blanc and Traminer was ruled out. The data proved asecond-degree relationship between Gouais blanc and Ribolla gialla and a third or more distant degree ofrelationships between Ribolla gialla and Traminer

Detection of grapevine synonyms in Lombardy and Piedmont regions (northern Italy)

The Italian viticulture is characterized by a high number of autochthonous varieties (about 1700), some of them renowned throughout the Country, some other only of local importance. Nevertheless, the real estimation of this biodiversity is made difficult due to the great amount of synonyms, homonyms and misnomers. The aim of this work was to undertake a comparison for varietal identity of minor, lesser cultivars in two field collections of neighboring regions (Lombardy and Piedmont) located in northern Italy, verifying synonyms in different areas. Forty-seven accessions were described by morphological (OIV descriptors) and genetic (microsatellites) characteristics. Thirty-eight varieties were identified belonging to three categories: varieties of great importance in Italy and locally misnamed, varieties important in the region of Piedmont and misnamed in Lombardy, and currently minor grapes but of historical significance in both Piedmont and Lombardy regions

Acute idiopathic pericarditis: current immunological theories

Alida LP Caforio,1 Renzo Marcolongo,2 Antonio Brucato,3 Luca Cantarini,4 Massimo Imazio,5 Sabino Iliceto11Division of Cardiology, Department of Cardiology, Thoracic and Vascular Sciences, University of Padua, Padua; 2Haematology and Clinical Immunology, Department of Medicine, University of Padua, Padua, 3Internal Medicine, Ospedali Riuniti, Bergamo, 4Rheumatology Unit, Policlinico Le Scotte, University of Siena, Siena, 5Department of Cardiology, Maria Vittoria Hospital, Torino, ItalyAbstract: Idiopathic recurrent acute pericarditis (IRAP) is a rare disease of suspected immune-mediated pathogenesis. It represents a diagnosis of exclusion. It is necessary to rule out infectious and noninfectious causes of pericardial inflammation, including systemic autoimmune and immune-related disorders, eg, Sjögren's disease, systemic lupus erythematosus. Since pericarditis may precede diagnosis of these disorders, IRAP diagnosis is often made after a long follow-up. According to the two main pathogenetic theories IRAP may represent an organ-specific autoimmune disease or an autoinflammatory disease (AInfD). The main evidence for autoimmunity in IRAP is provided by the detection of serum antiheart and antiintercalated-disk autoantibodies, and the response to anti-inflammatory or immunosuppressive therapy. The findings of familial forms and of proinflammatory cytokines in the pericardial fluid in IRAP would be in keeping with both organ-specific autoimmune disease and AInfD. In fact, AInfD are genetic disorders characterized by primary dysfunction of the innate immune system, due to mutations of genes involved in the regulation of the inflammatory response, in the absence of antigen specific T cells or autoantibodies. In AInfD there are active disease phases with raised non-cardiac specific inflammatory markers, such as C-reactive protein, as well as symptom-free intervals with possible C-reactive protein normalization. A minority of IRAP patients (6%) carry a mutation in the TNFRSF1A gene, encoding the receptor for tumor necrosis factor-alfa. This suggests that some IRAP patients may have an atypical or subclinical form of AInfD. Thus, IRAP may represent a syndrome with distinct pathogenetic mechanisms in different patients' subsets.Keywords: pericarditis, autoimmunity, autoantibodies, heart disease, immune factor

Detection of grapevine synonyms in Lombardy and Piedmont regions (northern Italy)

The Italian viticulture is characterized by a high number of autochthonous varieties (about 1700), some of them renowned throughout the Country, some other only of local importance. Nevertheless, the real estimation of this biodiversity is made difficult due to the great amount of synonyms, homonyms and misnomers. The aim of this work was to undertake a comparison for varietal identity of minor, lesser cultivars in two field collections of neighboring regions (Lombardy and Piedmont) located in northern Italy, verifying synonyms in different areas. Forty-seven accessions were described by morphological (OIV descriptors) and genetic (microsatellites) characteristics. Thirty-eight varieties were identified belonging to three categories: varieties of great importance in Italy and locally misnamed, varieties important in the region of Piedmont and misnamed in Lombardy, and currently minor grapes but of historical significance in both Piedmont and Lombardy regions

Molecular approach to assess the origin of cv. Marzemino

DNA marker analysis was used to determine the varietal identity of Marzemino accessions in public collections and private Italian vineyards; relationships among this varietal group and Vertzami, a traditional Greek cultivar, were also investigated through SSR and AFLP approaches. Molecular results strongly support the relationship among Vertzami cultivars growing in Greece, Marzemino and several Italian accessions selected on the basis of etymological similarity. SSR data exclude a direct descent of Marzemino, or other related Italian varieties, from Vertzami; on the other hand the level of similarity among Vertzami, Marzemino and some related varieties indicates a possible common ancestor. None of the accessions is considered as common ancestor but on the basis of genomic variability in the Marzemino group and of the relationships with the other Italian cultivars a probable Italian ancestor is supposed.

Genetic relationships among grapevine cultivars grown in Oltrepò Pavese (Italy)

In order to evaluate the genetic distance among 47 grapevine accessions, including major and minor local cultivars grown in Oltrepò pavese (Pavia, Italy), DNA has been analysed with the AFLP approach (VOS et al. 1995). The electrophoretic analysis of the products amplified by 3 primer combinations showed high polymorphism.Furthermore, considering that DNA markers for haploid, uniparentally inherited genomes, such as the chloroplast DNA, are important indicators of pedigree, CpSSR analysis was also performed and this identified cases of maternal common origin among the analysed cultivars. The results show that these molecular tools allow univocal genotype identification and that the analysed germplasm has a wide genetic dissimilarity. Results are coherent with the postulation of a polycentric origin for the Oltrepò pavese cultivars and of a multiple varietal flow from different viticultural regions. The molecular information gathered in this research is essential for the establishment of an appropriate presentation programme of autochthonous varieties

Vitis vinifera subsp. sylvestris and sativa; so far, so close: a 20 SSR based comparison of the two taxa

In Vitis vinifera L., the hypothesis of secondary domestication center, located along the wild progenitor distribution areas, is suggestive and credible even if up to now close parentage relationships between domestic (Vitis vinifera L. subsp. sativa (DC.) Hegi) and wild (Vitis vinifera L. subsp. sylvestris (Gmel.) Hegi) grapevines have not been detected, possibly due to century long separation of the two subspecies. The aim of this work was to verify the possibility of tracing a flow between the two compartments basing on molecular data and thanks to the availability of a huge dataset comprising 645 wild and more than 1400 cultivated samples. Twenty SSR loci were used to describe and genotype both sylvestris and sativa compartments. The sylvestris samples were all collected in the frame of a three year census in Italy and are representative of the Italian distribution range from north to south. The cultivated sativa accessions mainly (1231 samples) belong to the Vassal (INRA-Montpellier) collection, while the remaining (200) were selected in the frame of the Italian grapevine germplasm. Results highlighted a high level of genetic diversity for both wild and cultivated groups. STRUCTURE analysis clearly evidenced the separation of the two compartments and no first or second degree relationships were evidenced between the two subspecies

Vitis vinifera subsp. sylvestris and sativa; so far, so close: a 20 SSR based comparison of the two taxa

In Vitis vinifera L., the hypothesis of secondary domestication center, located along the wild progenitor distribution areas, is suggestive and credible even if up to now close parentage relationships between domestic (Vitis vinifera L. subsp. sativa (DC.) Hegi) and wild (Vitis vinifera L. subsp. sylvestris (Gmel.) Hegi) grapevines have not been detected, possibly due to century long separation of the two subspecies. The aim of this work was to verify the possibility of tracing a flow between the two compartments basing on molecular data and thanks to the availability of a huge dataset comprising 645 wild and more than 1400 cultivated samples. Twenty SSR loci were used to describe and genotype both sylvestris and sativa compartments. The sylvestris samples were all collected in the frame of a three year census in Italy and are representative of the Italian distribution range from north to south. The cultivated sativa accessions mainly (1231 samples) belong to the Vassal (INRA-Montpellier) collection, while the remaining (200) were selected in the frame of the Italian grapevine germplasm. Results highlighted a high level of genetic diversity for both wild and cultivated groups. STRUCTURE analysis clearly evidenced the separation of the two compartments and no first or second degree relationships were evidenced between the two subspecies