13,867 research outputs found

    Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene Therapy Model

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    Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model. For this purpose, we have chosen green fluorescent protein (GFP) as a target gene because the expression can be detected easily. GFP was inserted in a plasmid vector, pQE30 to develop the vector pQE30GFP. The plasmid pQE30GFP was constructed form plasmid, pQE30 and pEGFPC2. pQE30GFP injected directly in one group of fish into the muscle where luciferase expression was noted. In another group, after injection electroporation was performed where we have also noted luciferase expression; but, electroporation cause muscle injury to the zebrafish. In our case, the expression was very strong at the site of injection in first group in compare to electroporation group and in both the cases expression was stable more than two weeks

    Population Growth and Competition Models with Decay and Competition Consistent Delay

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    We derive an alternative expression for a delayed logistic equation in which the rate of change in the population involves a growth rate that depends on the population density during an earlier time period. In our formulation, the delay in the growth term is consistent with the rate of instantaneous decline in the population given by the model. Our formulation is a modification of [Arino et al., J.~Theoret.~Biol.~241(1):109--119, 2006] by taking the intraspecific competition between the adults and juveniles into account. We provide a complete global analysis showing that no sustained oscillations are possible. A threshold giving the interface between extinction and survival is determined in terms of the parameters in the model. The theory of chain transitive sets and the comparison theorem for cooperative delay differential equations are used to determine the global dynamics of the model. We extend our delayed logistic equation to a system modeling the competition of two species. For the competition model, we provide results on local stability, bifurcation diagrams, and adaptive dynamics. Assuming that the species with shorter delay produces fewer offspring at a time than the species with longer delay, we show that there is a critical value, Ļ„āˆ—\tau^*, such that the evolutionary trend is for the delay to approach Ļ„āˆ—\tau^*.Comment: 22 pages, 6 figures, 1 tabl

    Polychronicity, Decision-making and Entrepreneurial Self-efficacy of Venture Team Founders: An Exploratory Study

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    Purpose - This study explores the role of polychronic temporal orientation and decision-making decentralization on founders\u27 perceptions of entrepreneurial self-efficacy (ESE). Design/methodology/approach - Longitudinal survey data were collected from 141 business founders in China. Findings - Findings suggest that decision-making decentralization is positively associated with founders\u27 ESE. In addition, a polychronic temporal orientation is positively related to ESE, and this relationship is mediated by decision-making decentralization. Originality/value - This study adds to existing knowledge on ESE and temporal related issues by presenting empirical evidence that explains how and why the temporal orientation context and the practice of decision-making decentralization can shape ESE perceptions among venture founders

    Applying the selective Cu electroplating technique to light-emitting diodes

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    [[abstract]]We successfully fabricated a predefined patterned copper (Cu) substrate for thin GaN light-emitting diodes without barriers by the selective electroplating technique. The contours of Cu bumps fabricated using different electroplating modes and parameters were measured. We observed that the average thickness diminished with increasing current density. The current density conditions to obtain the best upright structure in the process were 40 and 80 mA/cm2.[[notice]]č£œę­£å®Œē•¢[[incitationindex]]SCI[[booktype]]ē“™ęœ¬[[booktype]]電子

    EFFECTIVENESS OF INTERGENERATIONAL PROGRAM IN IMPROVING COMMUNITY SENIOR CITIZENSā€™ GENERAL HEALTH AND HAPPINESS

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    Intergenerational programs (IGPs) encourage senior citizens tocontinue engaging in social participation, preserving their independence, andenriching their later life. However, IGPs in Taiwan (IGPs-T) are notprevalent and generally more apply between children and senior citizensexist only, which lack in-depth exchanges the senior citizenā€˜s life experienceand communication the young people. Relevant empirical results regardingthe effectiveness of IGPs activities on community senior citizensā€˜ health andhappiness are lacking. The objective of this study was to improve the mutualunderstanding and inclusion between community senior citizens and younggenerations, thereby improving the senior citizensā€˜ physical and mentalhealth functions and happiness.This was a two-group pre and post-test quasi-experimental studydesign and purposive sampling was used to recruit community senior citizensin Taipei City as research participants (N = 118). The experimental group (n= 55) received 90-min IGP-T activities once a week for 8 weeks. The controlgroup (n = 63) received general health services providing by communityhealth nurses in community.The outcomes of the experimental group significant progress in thehappiness (t=2.82, p=.007), and emotional well-being (EWB) (t=4.61,pā‰¦.001) from pre-test to post-test. And the experimental group wassignificantly superior to control group in perceived health status (PHS)(t=3.79, pā‰¦.001), happiness (t=3.82, pā‰¦.001), and emotional well-being(EWB) (t=4.93, pā‰¦.001) at post-test, and achieve a statistically significantdifference
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