The Effect of Juice Clarity and Several Conditions Promoting Yeast Growth on Fermentation Rate, the Production of Aroma Components and Wine Quality

In cellar practice grape juice is preferably made as clear as possible, and fermentations take place under strictly anaerobic conditions. In the laboratory, however, the fermentation rate of EK-filtered and de-aerated juices was always so much slower than that of juices obtained by normal cellar routine that no dry wines could be prepared unless fermentation stimulating methods were applied. Apparently in most cellar fermentations sufficient amounts of oxygen and/or of yeast growth promoting steroids from the grapes are present to complete fermentation, but the exact amounts present are unknown. In practice problems with lagging fermentation could occur in cases where clarifying and de-aeration of juice were performed too rigorously. Grape juice was clarified by diverse methods, and white wines were prepared from the juices under various conditions in order to study the effect of de·aeration, addition of settled juice with residual turbidity, micro-aeration and application of ergosterol suspensions on fermentation rate. EK-filtered juices were used to experiment with methods for reactivating lagging fermentation and also forinvestigating the effect of fermentation conditions on the production of wine volatiles. With the aid of the above-mentioned fermentation stimulating means the fermentation of EK-filtered juices could be completed within an acceptable period of time. Steps to reactivate lagging fermentations should be taken as early as possible, although additions of settled juice were often successful, even when applied at a late stage during fermentation. Filtered juice from different cultivars and origins behaved in a markedly similar way with regard to fermentation and the production of volatile components. Filtration of juice caused partial loss of the cultivar character of the wine but wines from filtered juice with additions of ergosterol were nevertheless allotted the highest scores during organoleptic evaluation. The production of higher alcohols depends on the degree of juice turbidity and fermentation temperature. In clear filtered juice, however, the production of higher alcohols was independent of fermentation temperature. The formation of acetates of higher alcohols is greatly enhanced, by adding ergosterol to the filtered juice

The effect of three years of TNF alpha blocking therapy on markers of bone turnover and their predictive value for treatment discontinuation in patients with ankylosing spondylitis: a prospective longitudinal observational cohort study

INTRODUCTION: The aim of this study was to investigate the effect of three years of tumor necrosis factor-alpha (TNF-alpha) blocking therapy on bone turnover as well as to analyze the predictive value of early changes in bone turnover markers (BTM) for treatment discontinuation in patients with ankylosing spondylitis (AS). METHODS: This is a prospective cohort study of 111 consecutive AS outpatients who started TNF-alpha blocking therapy. Clinical assessments and BTM were assessed at baseline, three and six months, as well as at one, two, and three years. Z-scores of BTM were calculated to correct for age and gender. Bone mineral density (BMD) was assessed yearly. RESULTS: After three years, 72 patients (65%) were still using their first TNF-alpha blocking agent. In these patients, TNF-alpha blocking therapy resulted in significantly increased bone-specific alkaline phosphatase, a marker of bone formation; decreased serum collagen-telopeptide (sCTX), a marker of bone resorption; and increased lumbar spine and hip BMD compared to baseline. Baseline to three months decrease in sCTX Z-score (HR: 0.394, 95% CI: 0.263 to 0.591), AS disease activity score (ASDAS; HR: 0.488, 95% CI: 0.317 to 0.752), and physician's global disease activity (HR: 0.739, 95% CI: 0.600 to 0.909) were independent inversely related predictors of time to treatment discontinuation because of inefficacy or intolerance. Early decrease in sCTX Z-score correlated significantly with good long-term response regarding disease activity, physical function and quality of life. CONCLUSIONS: Three years of TNF-alpha blocking therapy results in a bone turnover balance that favors bone formation, especially mineralization, in combination with continuous improvement of lumbar spine BMD. Early change in sCTX can serve as an objective measure in the evaluation of TNF-alpha blocking therapy in AS, in addition to the currently used more subjective measures

Factors affecting the reproducibility of fermentation of grape juice and of the aroma composition of wines 1. Grape maturity, sugar, inoculum concentration, aeration, juice turbidity and ergosterol

Gas chromatographic analysis was used for the investigation of some factors which determine the production of volatile wine components during fermentation. Especially the effect of "heterogenous" juice factors such as turbidity and air content were investigated in respect of fermentation rate and the production of fatty acids, fusel alcohols and esters. lt was shown that the fermentation lag of clear filtered and de-aerated grape juices could be prevented by small ergosterol additions before inoculation. A marked similarity was observed in the chromatograms of wines from fermentations of clear juices which were stimulated either by air or by ergosterol. Grape maturity proved to be a major factor affecting the production of volatile acids, alcohols and esters during fermentation. Fermentations of de-aerated and clear filtered juice with an addition of 0.5 g bentonite/l yielded wines with the best scores an sensory evaluation.Maßgebliche Faktoren für die Reproduzierbarkeit der Vergärung von Traubenmostund die Aromazusammensetzung von WeinI. Traubenreife, Zucker, Konzentration des lnokulums, Luft, Klarheit des Mostesund ErgosterinEinige Faktoren, welche die Bildung flüchtiger Weininhaltsstoffe bei der Gärung beeinflussen, wurden gaschromatographisch untersucht. Besonders die Wirkung „heterogener" Faktoren im Traubenmost, wie zum Beispiel Trübung und Luftgehalt, auf die Gärungsgeschwindigkeit und die Bildung von Fettsäuren, Fuselalkoholen und Estern wurde untersucht. Es wurde gezeigt, daß die Gärungsverzögerung bei klarfiltrierten und entlüfteten Traubensäften durch die Zufügung kleiner Mengen von Ergosterin vor der Inokulation verhindert werden kann. Wurde die Gärung von geklärtem Most durch Luft oder durch Ergosterin angeregt, so zeigten die Chromatogramme der Weine eine starke Ähnlichkeit. Die Traubenreife erwies sich als der wichtigste Faktor, der die Bildung von flüchtigen Säuren, Alkoholen und Estern bei der Gärung beeinflußt. Die Vergärung von entlüftetem und klarfiltriertem Most mit Zusatz von 0,5 g Bentonit/l ergab Weine, die bei der Weinprobe die höchsten Punktezahlen erhielten

Making the Shift: Narratives of Transfer from Planned Home Birth to the Hospital in the Pacific Northwest

About 1% of U.S. births each year are planned home births (Vital Statistics Reports 2017). Of these, roughly 10% transfer to a higher level of care during labor (Melissa Cheyney et al. 2014), yet their experiences are rarely heard. Storytelling has long been used not only as entertainment, but as a way of teaching and learning (Gidman 2013; Carter-Black 2007) and to help clinicians understand their patients’ experience of illness (Kleinman and Benson 2006; Brown and Closser 2016; Clarke, Jane Hanson, and Ross 2003). Using an experimental methodology inspired by both medical anthropology and the medical humanities, I share full narratives of transfer in order to amplify the voices of those who have experienced transfer immediately before or during labor; to highlight the responsible evaluation of risk involved in the decision to plan a home birth; and to offer an experience near analysis (Geertz 1974) for those who have not experienced a transfer of care during labor. In doing so, I aim to break down the binary of ‘home birther’ and ‘hospital birther’ to better facilitate smooth articulations (Robbie Davis-Floyd 2003) between patients and providers during transfer

Evaluation of sprayed & granular aphicides against the European asparagus aphid, Brachycolus asparagi (Homoptera: Aphididae) in British Columbia

A single spray of disulfoton and three of methamidophos (all at 1.12 kg ai/ha) suppressed populations of Brachycolus asparagi Mordvilko on immature asparagus for 90 days with light aphid damage. One application of oxydemeton-methyl (at 0.56 kg ai/ha) kept populations low as compared with the controls, but not low enough to prevent severe foliage damage. Two sprays of endosulfan (at 1.12 kg ai/ha) and three of malathion (at 2.24 kg ai/ha) during 90 days did not prevent moderate to heavy damage. The results indicated that the spray threshold of 0.5 B. asparagi/g of asparagus sprig used in this study was too high. No sprays controlled the other aphids present, which were mostly Myzus persicae (Sulzer). Granular formulations of disulfoton (at 0.5, 1.0, 2.0 and 4.0 kg ai/ha), carbofuran (at 2.0 kg ai/ha), and aldicarb (at 2.0 and 4.0 kg ai/ha) applied as side dressings alongside young asparagus, all gave excellent control for almost four months. CGA 73102 (at 1.0 and 2.0 kg ai/ha) and carbofuran (at 1.0 kg ai/ha) gave significant control for three months. Spray and granular treatments giving the best control also produced the thickest spears the following year

The 14C content of the ethanol of South African wines ior the years 1925-1975

It has been shown that, due to atmospheric conditions, South African natural wines are lower in 14C content than their northern hemisphere counterparts for the periods of active nuclear explosion tests. On this basis it is incorrect to apply northern hemisphere 14C data to wines of the southern hemisphere.Der 14C-Gehalt des Äthanols südafrikanischer Weine der Jahrgänge 1925-1975Es konnte gezeigt werden, daß südafrikanische Naturweine infolge der atmosphärischen Bedingungen einen niedrigeren 14C-Gehalt besitzen, als ihn Vergleichsweine von der nördlichen Erdhalbkugel aus der Zeit der Kernwaffenversuche aufweisen. Es ist somit nicht gerechtfertigt, die 14C-Werte der Nordhemisphäre bei Weinen der südlichen Erdhalbkugel anzuwenden

Effect-Directed Analysis of Municipal Landfill Soil Reveals Novel Developmental Toxicants in the Zebrafish Danio rerio

Effect-directed analysis (EDA) is an approach used to identify (unknown) contaminants in complex samples which cause toxicity, using a combination of biology and chemistry. The goal of this work was to apply EDA to identify developmental toxicants in soil samples collected from a former municipal landfill site. Soil samples were extracted, fractionated, and tested for developmental effects with an embryotoxicity assay in the zebrafish Danio rerio. Gas chromatograph mass selective detection (GC-MSD) chemical screening was used to reveal candidate developmental toxicants in fractions showing effects. In a parallel study, liquid chromatography-hybrid linear ion trap Orbitrap mass spectrometry was also applied to one polar subfraction (Hoogenboom et al. J. Chromatogr. A2009, 1216, 510-519). EDA resulted in the identification of a number of previously unknown developmental toxicants, which were confirmed to be present in soil by GC-MS. These included 11H-benzo[b]fluorene, 9-methylacridine, 4-azapyrene, and 2-phenylquinoline, as well as one known developmental toxicant (retene). This work revealed the presence of novel contaminants in the environment that may affect vertebrate development, which are not subject to monitoring or regulation under current soil quality assessment guidelines. © 2011 American Chemical Society

Histological Chages of Testis and Caput Epididymis in the Goat after Cannulation of the Rete Testis

雄ヤギ5頭を用い, 精巣網カテーテル装着手術後の精巣および精巣上体頭の組織構造を観察するとともに精巣静脈血中のテストステロン濃度を調べた。カテーテル装着手術後の精巣は, いずれも萎縮し, 正常な精子形成過程が観察される精細管は少なかった。また, ライディヒ細胞の形態や染色性には異常は認められなかった。一方, 精巣上体頭では, 精巣上体管が萎縮し, 主細胞の高さは無傷のものよりも有意に減少した。同様の組織構造の変化は, 精巣輸出管を切除した場合の精巣および精巣上体頭でも認められた。精巣静脈血中のテストステロン濃度は無傷の場合94.4ng/mlであり, カテーテル装着手術後では86.5∿342.8ng/mlであった。 / The testis and caput epididymis of the goat after cannulation of the rete testis or efferentiectomy were examined histologically. The concentration of testosterone in testicular venous blood collected from the same animals was also measured by radioimmunoassay. After cannulation, seminiferous tubules showed a sign of degeneration, although no morphological change of Leydig cells was observed. Epididymal ducts were severely atrophied and the height of principal epithelial cells was significantly decreased. A similar damage was observed in the testis and epididymis after efferentiectomy. When the efferent duct bundle had been incompletely ligated at cannulation, or rete testis catheter was kept in place until sampling time, damages in the testis were less severe. And in the former case the epididymis was impaired less severely. The concentration of testosterone in testicular venous blood collected from an intact animal was 94.4ng/ml, while that obtained after cannulation was 86.5-342.8ng/ml. These results suggest that degeneration of germ cells in seminiferous tubules observed after cannulation of the rete testis or efferentiectomy may be caused by stagnation of rete testicular fluid in the tubules, and that testosterone in the fluid plays important roles in the maintenance of morphology and function of the principal cells in the caput epididymis

Testing Consumer Rationality using Perfect Graphs and Oriented Discs

Given a consumer data-set, the axioms of revealed preference proffer a binary test for rational behaviour. A natural (non-binary) measure of the degree of rationality exhibited by the consumer is the minimum number of data points whose removal induces a rationalisable data-set.We study the computational complexity of the resultant consumer rationality problem in this paper. This problem is, in the worst case, equivalent (in terms of approximation) to the directed feedback vertex set problem. Our main result is to obtain an exact threshold on the number of commodities that separates easy cases and hard cases. Specifically, for two-commodity markets the consumer rationality problem is polynomial time solvable; we prove this via a reduction to the vertex cover problem on perfect graphs. For three-commodity markets, however, the problem is NP-complete; we prove thisusing a reduction from planar 3-SAT that is based upon oriented-disc drawings