247 research outputs found
Basic Study for Increasing Functional Food Ingredients Content in Tomato Using Genetic Diversity
Poster Presentation
Analysis of plant secondary metabolism using stable isotopeâlabelled precursors
Special issue of Phytochemical Analysis on NMR-based analytical techniques.
open access articleIntroduction
Analysis of biochemical pathways typically involves feeding a labelled precursor to an organism, and then monitoring the metabolic fate of the label. Initial studies used radioisotopes as a label and then monitored radioactivity in the metabolic products. As analytical equipment improved and became more widely available, preference shifted the use stable âheavyâ isotopes like deuterium (2H)â, carbonâ13 (13C)â and nitrogenâ15 (15N)âatoms as labels. Incorporation of the labels could be monitored by mass spectrometry (MS), as part of a hyphenated tool kits, e.g. Liquid chromatography (LC)âMS, gas chromatography (GC)âMS, LCâMS/MS. MS offers great sensitivity but the exact location of an isotope label in a given metabolite cannot always be unambiguously established. Nuclear magnetic resonance (NMR) can also be used to pick up signals of stable isotopes, and can give information on the precise location of incorporated label in the metabolites. However, the detection limit for NMR is quite a bit higher than that for MS.
Objectives
A number of experiments involving feeding stable isotopeâlabelled precursors followed by NMR analysis of the metabolites is presented. The aim is to highlight the use of NMR analysis in identifying the precise fate of isotope labels after precursor feeding experiments. As more powerful NMR equipment becomes available, applications as described in this review may become more commonplace in pathway analysis.
Conclusion and Prospects
NMR is a widely accepted tool for chemical structure elucidation and is now increasingly used in metabolomic studies. In addition, NMR, combined with stable isotope feeding, should be considered as a tool for metabolic flux analyses
Torons and black hole entropy
We consider a supersymmetric system of D-5-branes compactified on a 5-torus
with a self-dual background field strength on a 4-torus and carrying
left-moving momentum along a circle. The corresponding supergravity solution
describes a 5-dimensional black hole with a regular horizon. The entropy of
this black hole may be explained in terms of the Landau degeneracy for open
strings stretching between different branes. In the gauge theory approximation
this D-5-brane system is described by a super Yang-Mills theory with a t'Hooft
twist. By choosing a supersymmetric branch of the theory we obtain perfect
agreement with the entropy formula. The result relies on the number of massless
torons associated with the gauge field components that obey twisted boundary
conditions.Comment: 31 pages, latex. Some equations corrected. Final version to be
published in Nuclear Physics
A characterization of varieties whose universal cover is the polydisk or a tube domain
In this article we give necessary and sufficient conditions, in terms of certain tensors called semispecial tensors, respectively slope zero tensors, in order that the universal covering of a complex projective manifold be a symmetric domain of tube type. As an application, we give precisions of a result of Kazhdan showing that a Galois conjugate of such a manifold has the same universal coverin
Phosphorus removal from eutrophic waters with an aluminium hybrid nanocomposite
An excess of phosphorus (P) is the most common cause of eutrophication of freshwater bodies. Thus, it is imperative to reduce the concentration of P to prevent harmful algal blooms. Moreover, recovery of P has been gaining importance because its natural source will be exhausted in the near future. Therefore, the present work investigated the removal and recovery of phosphate from water using a newly developed hybrid nanocomposite containing aluminium nanoparticles (HPN). The HPN-Pr removes 0.80â±â0.01 mg P/g in a pH interval between 2.0 and 6.5. The adsorption mechanism was described by a Freundlich adsorption model. The material presented good selectivity for phosphate and can be regenerated using an HCl dilute solution. The factors that contribute most to the attractiveness of HPN-Pr as a phosphate sorbent are its moderate removal capacity, feasible production at industrial scale, reuse after regeneration and recovery of phosphate.The authors acknowledge the Foundation for Science and Technology (FCT) Project SFRH/BD/39085/2007 for the financial support
Clinicopathologic characteristics and prognostic factors of ovarian fibrosarcoma: the results of a multi-center retrospective study
<p>Abstract</p> <p>Background</p> <p>Ovarian fibrosarcomas are very rare tumors, and therefore, few case studies have evaluated the prognostic factors of this disease. To our knowledge, this study represents the largest study to evaluate the clinical and pathologic factors associated with ovarian fibrosarcoma patients.</p> <p>Methods</p> <p>Thirty-one cases of ovarian fibrosarcoma were retrospectively reviewed, which included medical records for eight patients, and 23 published case reports from 1995 through 2009. Patient treatment regimens included total hysterectomy with bilateral adnexectomy and an omentectomy (BAO) (n = 9), oophorectomy (OR) (n = 8), chemotherapy (CT) (n = 1), BAO followed by chemotherapy (BAO+CT) (n = 11), BAO followed by radiotherapy (BAO+RT) (n = 1), and oophorectomy followed by radiotherapy (OR + RT) (n = 1).</p> <p>Results</p> <p>The patients of this cohort were staged according to the guidelines of the Federation of Gynecology and Obstetrics (FIGO), with 15, 6, 9, and 1 stage I-IV cases identified, respectively. Mitotic count values were also evaluated from 10 high-power fields (HPFs), and 3 cases had an average mitotic count < 4, 18 cases were between 4 and 10, and 10 cases had an average mitotic count value ℠10. The Ki-67 (MIB-1) proliferation index values were grouped according to values that as follows: < 10% (n = 5), between 10% and 50% (n = 9), and ℠50% (n = 5). Positive expression of vimentin (100%, 22/22) and negative expression of CD117 (0%, 5/5) were also detected. Moreover, expression of smooth muscle actin (2/18), desmin (1/13), epithelial membrane antigen (0/11), S-100 (1/19), CD99 (0/6), CD34 (1/5), α-inhibin (7/15), estrogen receptor (1/6), and progesterone receptor (1/6) were reported for subsets of the cases examined. After a median follow-up period of 14 months (range, 2-120), the 2-year overall survival rates (OS) and disease-free survival (DFS) rates for all patients were 55.9% and 45.4%, respectively. Cox proportional hazard regression analysis of survival showed that FIGO stage (<it>P </it>= 0.007) and treatment (<it>P </it>= 0.008) were predictive of poor prognosis. Furthermore, patients with stage I tumors that received BAO+CT were associated with a better prognosis.</p> <p>Conclusions</p> <p>Mitotic activity, and cells positive for Ki-67 were identified as important factors in the diagnosis of ovarian fibrosarcoma. Furthermore, FIGO stage and treatment modalities have the potential to be prognostic factors of survival, with BAO followed by adjuvant chemotherapy associated with an improved treatment outcome.</p
Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans.
Collaboration with:
UniversitĂ© dâOrlĂ©ans, 28000 Chartres, France,
Université de Picardie Jules Verne, F-80037 Amiens, France
De Montfort University
Open access articleLinum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands
Rotational Surfaces in and Solutions in the Nonlinear Sigma Model
The Gauss map of non-degenerate surfaces in the three-dimensional Minkowski
space are viewed as dynamical fields of the two-dimensional O(2,1) Nonlinear
Sigma Model. In this setting, the moduli space of solutions with rotational
symmetry is completely determined. Essentially, the solutions are warped
products of orbits of the 1-dimensional groups of isometries and elastic curves
in either a de Sitter plane, a hyperbolic plane or an anti de Sitter plane. The
main tools are the equivalence of the two-dimensional O(2,1) Nonlinear Sigma
Model and the Willmore problem, and the description of the surfaces with
rotational symmetry. A complete classification of such surfaces is obtained in
this paper. Indeed, a huge new family of Lorentzian rotational surfaces with a
space-like axis is presented. The description of this new class of surfaces is
based on a technique of surgery and a gluing process, which is illustrated by
an algorithm.Comment: PACS: 11.10.Lm; 11.10.Ef; 11.15.-q; 11.30.-j; 02.30.-f; 02.40.-k. 45
pages, 11 figure
Three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (â)-pinoresinol via kinetic resolution
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