Micro, Meso, and Macro Data Collection and Analysis, as a Method for Speculative and Artistic Exploration

In this work, an attempt is made to explore the emerging computationally-enhanced private and public environments by analyzing their ecological transitions and its implications on practical, aesthetic, and speculative dimensions. The author has decided to methodologically dissect the multiplicity of information that exists on many possible-to-detect scales (micro, meso, macro), and utilize this extraction as a tool for experimentation and redefinition. With the use of custom-made hardware and software utilities (sensor devices, sentiment analysis algorithms, online APIs, and many more), a vast amount of data is collected and used as a multidimensional layered architecture that constantly shifts and transforms. The extracted and analyzed content of the collection becomes the essence of the work that is shaped and refined through digital and physical making – middleware, recursion, mapping – and by utilizing technological objects within the physical space, the creative process is augmented and amplified, exploring not only new practices and novel applications, but rather redefining behavior, thought-process, and context

Further development of a liquid chromatography–high‐resolution mass spectrometry/mass spectrometry‐based strategy for analyzing eight biomarkers in human urine indicating toxic mushroom or Ricinus communis

Recently, we presented a strategy for analysis of eight biomarkers in human urine to verify toxic mushroom or Ricinus communis ingestions. However, screening for the full panel is not always necessary. Thus, we aimed to develop a strategy to reduce analysis time and by focusing on two sets of analytes. One set (A) for biomarkers of late-onset syndromes, such as phalloides syndrome or the syndrome after castor bean intake. Another set (B) for biomarkers of early-onset syndromes, such as pantherine–muscaria syndrome and muscarine syndrome. Both analyses should be based on hydrophilic-interaction liquid chromatography coupled with high-resolution mass spectrometry (MS)/MS (HILIC-HRMS/MS). For A, urine samples were prepared by liquid–liquid extraction using dichloromethane and subsequent solid-phase extraction of the aqueous supernatant. For B urine was precipitated using acetonitrile. Method A was validated for ricinine and α- and β-amanitin and method B for muscarine, muscimol, and ibotenic acid according to the specifications for qualitative analytical methods. In addition, robustness of recovery and normalized matrix factors to matrix variability measured by urinary creatinine was tested. Moreover, applicability was tested using 10 urine samples from patients after suspected mushroom intoxication. The analytes α- and β-amanitin, muscarine, muscimol, and ibotenic acid could be successfully identified. Finally, psilocin-O-glucuronide could be identified in two samples and unambiguously distinguished from bufotenine-O-glucuronide via their MS2 patterns. In summary, the current workflow offers several advantages towards the previous method, particularly being more labor-, time-, and cost-efficient, more robust, and more sensitive