Real-time RT-PCR quantitative analysis of plant viruses in stone fruit tissues

Real-time PCR assays aiming at quantifying the level of plant infection by pathogens are becoming more and important. Within microbiology, the application of Real-time PCR has had the biggest impact upon the field of virology. However, Real-time PCR application in fundamental plant virology studies is still lagging behind. The use of relative and absolute quantification is discussed in this study. Also, case studies including Plum pox virus in transgenic plums C5 are presented.Keywords: Plum pox virus, C5, Real-time PCR, quantitatio

Evaluation of transgenic Prunus domestica L., clone C5 resistance to Plum pox virus

Plum pox virus (PPV) is one of the most devastating diseases of Prunus species. Since few sources of resistance to PPV have been identified, transgene-based resistance offers a complementary approach to developing PPV-resistant stone fruit cultivars. C5, a transgenic clone of Prunus domestica L., containing the PPV coat protein (CP) gene, has been described as highly resistant to PPV in greenhouse tests, displaying characteristics typical of post-transcriptional gene silencing (PTGS). Moreover, C5 trees exposed to natural aphid vectors in the field remained uninfected after 4 years while susceptible transgenic and untransformed trees developed severe symptoms within the first year. In our study, a high and permanent infection pressure of PPV was provided by bud grafting of inoculum in the field trial of clone C5 conducted in the Czech Republic, in which PPV-infected and healthy control trees were used. Moreover, trees with combined inoculations by PPV, ACLSV and PDV were also used in the trial. The presence of the viruses throughout the tree tissues, the relative titre of the viruses and symptoms on C5 trees have been monitored over the years. The resistance stability of C5 clones under permanent infection pressure is discussed.Keywords: PPV, C5, resistance, real-time PC

The synthesis of SiC and TiC protective coatings for carbon fibers by the reactive replica process

International audienc

Discrimination of three BYDV species by one-step RT-PCR-RFLP and sequence based methods in cereal plants from the Czech Republic

One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. RFLP analysis performed on 23 samples including one lab isolate showed three types of Hpa II restriction profile. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. One-step RT-PCR together with RFLP presents an easy and reliable assay for routine BYDV typing. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic

Adsorption de micropolluants sur tissus et feutres de carbone activé

National audienc

Thermodynamic study of seven micropollutants adsorption onto an activated carbon cloth: Van’t Hoff method, calorimetry, and COSMO-RS simulations

International audienc

Adsorption of micropollutants onto activated carbon fabric and felt

International audienc

A friendly detergent for H2 oxidation by Aquifex aeolicus membrane-bound hydrogenase immobilized on graphite and Self-Assembled-Monolayer-modified gold electrodes

International audienc

Oxidation behavior at moderate temperature under dry and wet air of phenolic resin-derived carbon

International audienceThe oxidation behavior of carbon materials has been investigated at temperatures less than 773 K using isothermal thermogravimetric analyses under dry and wet air. Bulk samples obtained by the pyrolysis of phenolic resin have been characterized using X-ray diffraction and elementary analysis. X-ray photoelectron spectroscopy and Raman spectroscopy have been performed to analyze the surface and subsurface. The carbon structure has a poor organization state and a gradient in the oxygen concentration and structure between the surface and the bulk is revealed. The material is more reactive under wet air than dry air in terms of oxidation rates as determined between 623 and 773 K. The apparent activation energies are respectively 122 ± 6 kJ/mol in dry air and 102 ± 6 kJ/mol in wet air

How to advance the frontiers of current biofuel cells: design of a H2/O2 biofuel cell based on thermostable enzymes

International audienceA new generation of mediatorless H2/O2 biofuel cells was designed based on a hyperthermophilic O2-tolerant hydrogenase and a thermostable bilirubin oxidase both immobilized on carbon nanofibers. A power density up to 1.5 ± 0.2 mW.cm-2 at 60°C was reached. This first demonstration of a H2/O2 biofuel cell able to deliver electricity over a wide range of temperatures, from 30°C up to 80°C, and over a large pH window, allows considering this device as an alternative power supply for small portable applications in various environments, including extreme ones