Tracking Cats: Problems with Placing Feline Carnivores on δ18O, δD Isoscapes

Several felids are endangered and threatened by the illegal wildlife trade. Establishing geographic origin of tissues of endangered species is thus crucial for wildlife crime investigations and effective conservation strategies. As shown in other species, stable isotope analysis of hydrogen and oxygen in hair (δD(h), δ(18)O(h)) can be used as a tool for provenance determination. However, reliably predicting the spatial distribution of δD(h) and δ(18)O(h) requires confirmation from animal tissues of known origin and a detailed understanding of the isotopic routing of dietary nutrients into felid hair.We used coupled δD(h) and δ(18)O(h) measurements from the North American bobcat (Lynx rufus) and puma (Puma concolor) with precipitation-based assignment isoscapes to test the feasibility of isotopic geo-location of felidae. Hairs of felid and rabbit museum specimens from 75 sites across the United States and Canada were analyzed. Bobcat and puma lacked a significant correlation between H/O isotopes in hair and local waters, and also exhibited an isotopic decoupling of δ(18)O(h) and δD(h). Conversely, strong δD and δ(18)O coupling was found for key prey, eastern cottontail rabbit (Sylvilagus floridanus; hair) and white-tailed deer (Odocoileus virginianus; collagen, bone phosphate).Puma and bobcat hairs do not adhere to expected pattern of H and O isotopic variation predicted by precipitation isoscapes for North America. Thus, using bulk hair, felids cannot be placed on δ(18)O and δD isoscapes for use in forensic investigations. The effective application of isotopes to trace the provenance of feline carnivores is likely compromised by major controls of their diet, physiology and metabolism on hair δ(18)O and δD related to body water budgets. Controlled feeding experiments, combined with single amino acid isotope analysis of diets and hair, are needed to reveal mechanisms and physiological traits explaining why felid hair does not follow isotopic patterns demonstrated in many other taxa

Resting energy expenditure and body composition of Labrador Retrievers fed high fat and low fat diets

A high dietary fat intake may be an important environmental factor leading to obesity in some animals. The mechanism could be either an increase in caloric intake and/or a decrease in energy expenditure. To test the hypothesis that high fat diets result in decreased resting energy expenditure (REE), we measured REE using indirect calorimetry in 10-adult intact male Labrador Retrievers, eating weight-maintenance high-fat (HF, 41% energy, average daily intake: 8018 +/- 1247 kJ/day, mean +/- SD) and low-fat (LF, 14% energy, average daily intake: 7331 +/- 771 kJ/day) diets for a 30-day period. At the end of each dietary treatment, body composition measurements were performed using dual-energy X-ray absorptiometry. The mean +/- SD REE was not different between diets (4940 +/- 361 vs. 4861 +/- 413 kJ/day on HF and LF diets respectively). Measurements of fat-free mass (FFM) and fat mass (FM) also did not differ between diets (FFM: 26.8 +/- 2.3 kg vs. 26.3 +/- 2.5 kg; FM: 3.0 +/- 2.3 vs. 3.1 +/- 1.5 kg on HF and LF diets respectively). In summary, using a whole body calorimeter, we found no evidence of a decrease in REE or a change in body composition on a HF diet compared with LF diet

Mesozoic sedimentary cover sequences of the Congo Basin in the Kasai region, Democratic Republic of Congo

The Congo Basin represents one of the largest and least studied continental sedimentary basins in the world. The stratigraphy of cover sequences across the basin is poorly resolved and a somewhat simple stratigraphy has generally been applied with gross subdivision of the Mesozoic-Cenozoic cover sequences into a number weakly correlated units. Although these subdivisions are useful for broad, regional-scale correlations, investigation of drill cores and outcrop in the shallow, southern Kasai part of the basin, from Tshikapa to Kabinda, reveals considerable facies, provenance and thickness variations, suggesting a more complex depositional and stratigraphic history than previously recognized. This study now permits the subdivision of the sedimentary cover in the Kasai portion of the Congo Basin into five distinct depositional sequences consisting of (1) P1: Permo-Carboniferous glacio-lacustrine deposits correlative to the Lukuga Group; (2) J1: Jurassic-age arid to semi-arid laminated shales and siltstones and aeolian sandstones, interpreted as ephemeral lake and sand dune sequences with interspersed loess deposits and rare fluvial channel sequences (considered part of the historic Lualaba-Lubilash Supergroup—the lacustrine facies likely correlates with the Stanleyville Group, DRC and the Continental Intercalar Group, Angola); (3) C1 & C2: Lower Cretaceous locally heavy mineral-rich fluvial sandstone deposits and variably present basal conglomerate (correlated to the Loia Group, DRC and the Calonda Formation, Angola); (4) C3 & C4: Upper Cretaceous conglomerates of alluvial fan origin that grade upward into laminated shales and siltstones or well-sorted and rounded, fined grained sandstones representative of a semi-arid to arid depositional setting dominated by ephemeral lakes and small aeolian dunes, (equated to the Kwango Group, DRC and Angola) and (5) T1: fluvial, aeolian and lacustrine sediments of Paleogene age (correlated with portions of the Kalahari Group). The results convincingly suggest that this part of the Congo Basin is more structurally complex than previously appreciated, with multiple fault-bounded basement highs and depocenters that strongly influenced regional sedimentation patterns. Prolonged and sporadic displacement appears to have taken place along these faults, leading to heavily bisected basin morphology with uneven thickness and depth distributions between sequences. The deposition of Cretaceous sequences was coeval with two episodes of kimberlite emplacement, the first at ~120–130 Ma in northern Angola, and the second at ~70–80 Ma in the DRC, with gravel horizons within the Cretaceous fluvial successions (C1 and C3) known for their alluvial diamond concentration. The models developed provide a regional context for evaluation of alluvial diamond source areas and prospectivity