PCR detection of Burkholderia multivorans in water and soil samples

Background: Although semi-selective growth media have been developed for the isolation of Burkholderia cepacia complex bacteria from the environment, thus far Burkholderia multivorans has rarely been isolated from such samples. Because environmental B. multivorans isolates mainly originate from water samples, we hypothesized that water rather than soil is its most likely environmental niche. The aim of the present study was to assess the occurrence of B. multivorans in water samples from Flanders (Belgium) using a fast, culture-independent PCR assay. Results: A nested PCR approach was used to achieve high sensitivity, and specificity was confirmed by sequencing the resulting amplicons. B. multivorans was detected in 11 % of the water samples (n = 112) and 92 % of the soil samples (n = 25) tested. The percentage of false positives was higher for water samples compared to soil samples, showing that the presently available B. multivorans recA primers lack specificity when applied to the analysis of water samples. Conclusions: The results of the present study demonstrate that B. multivorans DNA is commonly present in soil samples and to a lesser extent in water samples in Flanders (Belgium)

The taste for the particular: A logic of discernment in an age of omnivorousness

This article provides an analysis of two leading specialist wine magazines, Decanter and Wine Spectator, and the codification and legitimation of a ‘taste for the particular.’ Such media of connoisseurship are key institutions of evaluation and legitimation in an age of omnivorousness, but are often overlooked in research that foregrounds the agency of tasters and neglects the conventionalization of tasting norms and devices. The wine field has undergone a process of democratization typical of omnivorousness more broadly: former elite/low boundaries (operationalized in the paper through the Old/New World dichotomy) are ignored, and a discerning attitude is encouraged for wines from a diversity of regions. Drawing on the magazines’ audience profile and market position data, and a content analysis of advertising and editorial content from 2008 and 2010, I examine the differences in the use of four legitimation frames (transparency, heritage, genuineness and external validation) for the provenance elements of Old and New World wines. The analysis suggests that the Old World—typically French—notion of terroir, on which the traditional Old/New World boundary rested, has been democratized through the particularities of provenance. Yet, the analysis also reveals continuing differences between the two categories (including greater emphasis on the heritage and external validation of Old World context of production, and on the transparency and genuineness of New World producers), and the preservation of established hierarchies of taste through the application of terroir to New World wines, which retain the Old World and France as their master referent

Co-creating, co-producing and connecting: Museum practice today.

This is the peer reviewed version of the following article: Barnes, P., & McPherson, G. (2019). Co‐Creating, Co‐producing and Connecting: Museum Practice Today. Curator: The Museum Journal, 62(2), 257-267., which has been published in final form at https://doi.org/10.1111/cura.12309. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-ArchivingWe argue in this paper that museums have become hybrid spaces, where consumers look and challenge what they see; they form part of what they see; with some aspects of exhibitions now co‐created and co‐produced by the consumer (Kershaw et al. 2018; Solis 2012). This paper draws on an example from a group that we worked with using performance as a tool to engage a ‘hard to reach’ or ‘socially excluded’ groups. We conclude that by allowing audiences to co‐create and co‐produce exhibitions and performance; this can turn the museum rhetoric of community engagement into practice and create a space that is truly inclusive for the communities it serves. We demonstrate how the possibility of seeing museums as hybrid spaces, which can adapt, can be used for education and entertainment, and how that has in turn led to the transformation of people's lives in a previously socially excluded community

Reading behaviour from adolescence to early adulthood: A panel study of the impact of family and education on reading fiction books

In this article we study how the frequency of book-reading - a form of legitimate culture - develops in the period from adolescence to young adulthood and how it is influenced by parents' education, parental reading socialization climate, school and their interactions. In disentangling parental and educational effects we contribute to the cultural reproduction-cultural mobility debate. We use multi-actor panel data on three cohorts of Dutch secondary school students (and their parents) who took part in a classroom survey between the ages of 14 and 17, and who participated in at least one of the follow-up surveys two, four and six years later. We find that the amount of book-reading is more strongly associated with education than with parents' reading socialization. The influence of parents increases slightly in the period from adolescence to young adulthood. Differences in reading behaviour between students of different educational programmes increase during secondary education, but decrease in the period after secondary schooling. The transition to tertiary education hardly affects the frequency of reading. Overall, the results are more in line with the cultural reproduction model than with the cultural mobility model. © The Author(s) 2012

How people organise cultural attitudes : cultural belief systems and the populist radical right

Political scientists generally agree that all individuals structure their cultural attitudes in the same unidimensional fashion. However, various populist radical right parties remarkably combine moral progressiveness with conservatism regarding immigration-related issues. This suggests that the structuring of cultural attitudes among the electorate may also be more complex than typically assumed. Applying Correlational Class Analysis to representative survey data, the study uncovers three cultural belief systems. For individuals adhering to an integrated one, all cultural attitudes are interdependent, as typically assumed. However, two alternative belief systems are also uncovered: intermediate and partitioned. In the latter, positions on one cultural attitude (e.g. ethnocentrism) are barely related to positions on others (e.g. rejecting Islam or opposing homosexuality). The existence of multiple cultural belief systems challenges the widely held assumption that all people organise their cultural attitudes similarly. Both political party agendas and individuals’ education level and religion appear key to understanding variation in belief systems