Modeling the Transport and Deposition of ¹⁰Be Produced by the Strongest Solar Proton Event During the Holocene

Prominent excursions in the number of cosmogenic nuclides (e.g., ¹⁰Be) around 774 CE/775 document the most severe solar proton event (SPE) throughout the Holocene. Its manifestation in ice cores is valuable for geochronology, but also for solar-terrestrial physics and climate modeling. Using the ECHAM/MESSy Atmospheric Chemistry (EMAC) climate model in combination with the Warning System for Aviation Exposure to SEP (WASAVIES), we investigate the transport, mixing, and deposition of the cosmogenic nuclide ¹⁰Be produced by the 774 CE/775 SPE. By comparing the model results to the reconstructed ¹⁰Be time series from four ice core records, we study the atmospheric pathways of ¹⁰Be from its stratospheric source to its sink at Earth's surface. The reconstructed post-SPE evolution of the ¹⁰Be surface fluxes at the ice core sites is well captured by the model. The downward transport of the ¹⁰Be atoms is controlled by the Brewer-Dobson circulation in the stratosphere and cross-tropopause transport via tropopause folds or large-scale sinking. Clear hemispheric differences in the transport and deposition processes are identified. In both polar regions the ¹⁰Be surface fluxes peak in summertime, with a larger influence of wet deposition on the seasonal ¹⁰Be surface flux in Greenland than in Antarctica. Differences in the peak ¹⁰Be surface flux following the 774 CE/775 SPE at the drilling sites are explained by specific meteorological conditions depending on the geographic locations of the sites

Ciliary Beating Recovery in Deficient Human Airway Epithelial Cells after Lentivirus Ex Vivo Gene Therapy

Primary Ciliary Dyskinesia is a heterogeneous genetic disease that is characterized by cilia dysfunction of the epithelial cells lining the respiratory tracts, resulting in recurrent respiratory tract infections. Despite lifelong physiological therapy and antibiotics, the lungs of affected patients are progressively destroyed, leading to respiratory insufficiency. Recessive mutations in Dynein Axonemal Intermediate chain type 1 (DNAI1) gene have been described in 10% of cases of Primary Ciliary Dyskinesia. Our goal was to restore normal ciliary beating in DNAI1–deficient human airway epithelial cells. A lentiviral vector based on Simian Immunodeficiency Virus pseudotyped with Vesicular Stomatitis Virus Glycoprotein was used to transduce cultured human airway epithelial cells with a cDNA of DNAI1 driven by the Elongation Factor 1 promoter. Transcription and translation of the transduced gene were tested by RT–PCR and western blot, respectively. Human airway epithelial cells that were DNAI1–deficient due to compound heterozygous mutations, and consequently had immotile cilia and no outer dynein arm, were transduced by the lentivirus. Cilia beating was recorded and electron microscopy of the cilia was performed. Transcription and translation of the transduced DNAI1 gene were detected in human cells treated with the lentivirus. In addition, immotile cilia recovered a normal beat and outer dynein arms reappeared. We demonstrated that it is possible to obtain a normalization of ciliary beat frequency of deficient human airway epithelial cells by using a lentivirus to transduce cells with the therapeutic gene. This preliminary step constitutes a conceptual proof that is indispensable in the perspective of Primary Ciliary Dyskinesia's in vivo gene therapy. This is the first time that recovery of cilia beating is demonstrated in this disease

Dendrochronological potential and productivity of tropical tree species in Western Kenya

This study focuses on tropical tree growth rates in Western Kenya. The dendrochronological potential of each study species was determined by visual examination of rings, and then cumulative growth trajectories for diameter were synthesized for species of sufficient sample size (n ≥ 3), based on ring-width chronologies. The 14 tree species considered were: Acacia mearnsii, Bridelia micrantha, Combretum molle, Croton macrostachyus, Cupressus lustianica, Eucalyptus camaldulensis, Eucalyptus grandis, Eucalyptus saligna, Grevillea robusta, Mangifera indica, Markhamia lutea, Persia Americana, Syzygium cumini, and Trilepisium madagascariensis. The species with the highest dendrochronological potential included Acacia mearnsii, Cupressus lusitanica, the Eucalyptus spp. and Mangifera indica, which are all non-native species that successfully crossdated. The results also indicated that the species with highest dendrochronological potential had strong radial growth synchrony, which was reflected in high inter-tree correlation and (or) high growth variance explained by the first principal component axis. Furthermore, A. mearnsii and E. camaldulensis were sensitive to annual precipitation and moisture index. The species with the lowest dendrochronological potential were Grevillea robusta and Markhamia lutea. In terms of productivity, the three fastest growing species in the study, based on annual diameter increment, were Eucalyptus camaldulensis, Eucalyptus grandis, and Acacia mearnsii. This study also has great potential to extrapolate historical patterns of diameter growth to understanding annual aboveground biomass and carbon dynamics in Western Kenya.This item is part of the Tree-Ring Research (formerly Tree-Ring Bulletin) archive. For more information about this peer-reviewed scholarly journal, please email the Editor of Tree-Ring Research at [email protected]

Quantitative measurements of free and immobilized RgDAAO Michaelis-Menten constant using an electrochemical assay reveal the impact of covalent cross-linking on substrate specificity

Challenges facing enzyme-based electrochemical sensors include substrate specificity, batch to batch reproducibility, and lack of quantitative metrics related to the effect of enzyme immobilization. We present a quick, simple, and general approach for measuring the effect of immobilization and cross-linking on enzyme activity and substrate specificity. The method can be generalized for electrochemical biosensors using an enzyme that releases hydrogen peroxide during its catalytic cycle. Using as proof of concept RgDAAO-based electrochemical biosensors, we found that the Michaelis-Menten constant (Km) decreases post immobilization, hinting at alterations in the enzyme kinetic properties and thus substrate specificity. We confirm the decrease in Km electrochemically by characterizing the substrate specificity of the immobilized RgDAAO using chronoamperometry. Our results demonstrate that enzyme immobilization affects enzyme substrate specificity and this must be carefully evaluated during biosensor development. Graphical abstract: [Figure not available: see fulltext.

Modeling the Transport and Deposition of 10Be Produced by the Strongest Solar Proton Event During the Holocene

Prominent excursions in the number of cosmogenic nuclides (e.g., 10Be) around 774 CE/775 document the most severe solar proton event (SPE) throughout the Holocene. Its manifestation in ice cores is valuable for geochronology, but also for solar-terrestrial physics and climate modeling. Using the ECHAM/MESSy Atmospheric Chemistry (EMAC) climate model in combination with the Warning System for Aviation Exposure to SEP (WASAVIES), we investigate the transport, mixing, and deposition of the cosmogenic nuclide 10Be produced by the 774 CE/775 SPE. By comparing the model results to the reconstructed 10Be time series from four ice core records, we study the atmospheric pathways of 10Be from its stratospheric source to its sink at Earth's surface. The reconstructed post-SPE evolution of the 10Be surface fluxes at the ice core sites is well captured by the model. The downward transport of the 10Be atoms is controlled by the Brewer-Dobson circulation in the stratosphere and cross-tropopause transport via tropopause folds or large-scale sinking. Clear hemispheric differences in the transport and deposition processes are identified. In both polar regions the 10Be surface fluxes peak in summertime, with a larger influence of wet deposition on the seasonal 10Be surface flux in Greenland than in Antarctica. Differences in the peak 10Be surface flux following the 774 CE/775 SPE at the drilling sites are explained by specific meteorological conditions depending on the geographic locations of the sites

Dendroclimatic analysis of white pine (Pinus strobus L.) using long-term provenance test sites across eastern North America

Abstract Background The main objective of this study was to examine the climatic sensitivity of the radial growth response of 13 eastern white pine (Pinus strobus L.) provenances planted at seven test sites throughout the northern part of the species’ native distribution in eastern North America. Methods The test sites (i.e., Wabeno, Wisconsin, USA; Manistique, Michigan, USA; Pine River, Michigan, USA; Newaygo, Michigan, USA; Turkey Point, Ontario, Canada; Ganaraska, Ontario, Canada; and Orono, Maine, USA) examined in this study were part of a range-wide white pine provenance trial established in the early 1960s in the eastern United States and Canada. Principal components analysis (PCA) was used to examine the main modes of variation [first (PC1) and second (PC2) principal component axes] in the standardized radial growth indices of the provenances at each test site. The year scores for PC1 and PC2 were examined in relation to an array of test site climate variables using multiple regression analysis to examine the commonality of growth response across all provenances to the climate of each test site. Provenance loadings on PC1 and PC2 were correlated with geographic parameters (i.e., latitude, longitude, elevation) and a suite of biophysical parameters associated with provenance origin location. Results The amount of variation in radial growth explained by PC1 and PC2 ranged from 43.4% to 89.6%. Dendroclimatic models revealed that white pine radial growth responses to climate were complex and differed among sites. The key dendroclimatic relationships observed included sensitivity to high temperature in winter and summer, cold temperature in the spring and fall (i.e., beginning and end of the growing season), summer moisture stress, potential sensitivity to storm-induced damage in spring and fall, and both positive and negative effects of higher winter snowfall. Separation of the loadings of provenances on principal component axes was mainly associated with temperature-related bioclimatic parameters of provenance origin at 5 of the 7 test sites close to the climate influence of the Great Lakes (i.e., Wabeno, Manistique, Pine River, Newaygo, and Turkey Point). In contrast, differences in radial growth response to climate at the Ganaraska test site, were driven more by precipitation-related bioclimatic parameters of the provenance origin location while radial growth at the easternmost Orono test site was independent of bioclimate at the provenance origin location. Conclusions Study results suggest that genetic adaptation to temperature and precipitation regime may significantly influence radial growth performance of white pine populations selected for use in assisted migration programs to better adapt white pine to a future climate

Dendroclimatic analysis of white pine (Pinus strobus L.) using long-term provenance test sites across eastern North America

Background: The main objective of this study was to examine the climatic sensitivity of the radial growth response of 13 eastern white pine (Pinus strobus L.) provenances planted at seven test sites throughout the northern part of the species’ native distribution in eastern North America. Methods: The test sites (i.e., Wabeno, Wisconsin, USA; Manistique, Michigan, USA; Pine River, Michigan, USA; Newaygo, Michigan, USA; Turkey Point, Ontario, Canada; Ganaraska, Ontario, Canada; and Orono, Maine, USA) examined in this study were part of a range-wide white pine provenance trial established in the early 1960s in the eastern United States and Canada. Principal components analysis (PCA) was used to examine the main modes of variation [first (PC1) and second (PC2) principal component axes] in the standardized radial growth indices of the provenances at each test site. The year scores for PC1 and PC2 were examined in relation to an array of test site climate variables using multiple regression analysis to examine the commonality of growth response across all provenances to the climate of each test site. Provenance loadings on PC1 and PC2 were correlated with geographic parameters (i.e., latitude, longitude, elevation) and a suite of biophysical parameters associated with provenance origin location. Results: The amount of variation in radial growth explained by PC1 and PC2 ranged from 43.4% to 89.6%. Dendroclimatic models revealed that white pine radial growth responses to climate were complex and differed among sites. The key dendroclimatic relationships observed included sensitivity to high temperature in winter and summer, cold temperature in the spring and fall (i.e., beginning and end of the growing season), summer moisture stress, potential sensitivity to storm- induced damage in spring and fall, and both positive and negative effects of higher winter snowfall. Separation of the loadings of provenances on principal component axes was mainly associated with temperature-related bioclimatic parameters of provenance origin at 5 of the 7 test sites close to the climate influence of the Great Lakes (i.e., Wabeno, Manistique, Pine River, Newaygo, and Turkey Point). In contrast, differences in radial growth response to climate at the Ganaraska test site, were driven more by precipitation-related bioclimatic parameters of the provenance origin location while radial growth at the easternmost Orono test site was independent of bioclimate at the provenance origin location. Conclusions: Study results suggest that genetic adaptation to temperature and precipitation regime may significantly influence radial growth performance of white pine populations selected for use in assisted migration programs to better adapt white pine to a future climate

Growth-climate relationships of Pinus strobusin the floodway versus terrace forest along the banks of the Red Cedar River, Michigan

We examined the effects of climate (i.e. temperature, precipitation, moisture index, river discharge) on the radial growth of eastern white pine (Pinus strobus) in two contrasting microhabitats (floodway versus terrace) in relation to an elevational and moisture gradient along the banks of the Red Cedar River, Michigan. We hypothesized that trees growing on the terrace sites would have a greater sensitivity to climatic factors than trees growing in the floodway. Increment cores were sampled and crossdated from dominant and co-dominant trees in the floodway and terrace sites and standardized growth chronologies were developed. Mean sensitivity, standard deviation, percentage of absent rings, and intertree correlation were generally greater for eastern white pine on the terrace compared to the floodway forests. Dendroclimatic relationships were examined with bootstrapped correlation analysis, which indicated that radial growth of eastern white pine in both floodway and terrace sites was negatively associated with summer temperature (i.e. June and July) of the current growing season. Radial growth in both floodway and terrace sites were positively related to current May precipitation, moisture index, and river discharge. Dendroclimatic response of eastern white pine in floodway and terrace sites differed in that the effect of summer moisture stress was more persistent in the terrace sites into later summer (i.e. current July ) whereas floodway sites showed a negative response to prior-year May precipitation and moisture index. Although precipitation and hydrological conditions of the river were generally favorable during the summer months, which appeared to have promoted soil moisture recharge, flooding in early winter (i.e. prior November) had a negative impact on growth in both microhabitats. The effect of winter river discharge was significantly negative in January (t) in the floodway sites compared to the terrace sites.This item is part of the Tree-Ring Research (formerly Tree-Ring Bulletin) archive. For more information about this peer-reviewed scholarly journal, please email the Editor of Tree-Ring Research at [email protected]

Modeling the Transport and Deposition of 10Be Produced by the Strongest Solar Proton Event During the Holocene

Prominent excursions in the number of cosmogenic nuclides (e.g., 10Be) around 774 CE/775 document the most severe solar proton event (SPE) throughout the Holocene. Its manifestation in ice cores is valuable for geochronology, but also for solar‐terrestrial physics and climate modeling. Using the ECHAM/MESSy Atmospheric Chemistry (EMAC) climate model in combination with the Warning System for Aviation Exposure to SEP (WASAVIES), we investigate the transport, mixing, and deposition of the cosmogenic nuclide 10Be produced by the 774 CE/775 SPE. By comparing the model results to the reconstructed 10Be time series from four ice core records, we study the atmospheric pathways of 10Be from its stratospheric source to its sink at Earth's surface. The reconstructed post‐SPE evolution of the 10Be surface fluxes at the ice core sites is well captured by the model. The downward transport of the 10Be atoms is controlled by the Brewer‐Dobson circulation in the stratosphere and cross‐tropopause transport via tropopause folds or large‐scale sinking. Clear hemispheric differences in the transport and deposition processes are identified. In both polar regions the 10Be surface fluxes peak in summertime, with a larger influence of wet deposition on the seasonal 10Be surface flux in Greenland than in Antarctica. Differences in the peak 10Be surface flux following the 774 CE/775 SPE at the drilling sites are explained by specific meteorological conditions depending on the geographic locations of the sites.Plain Language Summary: During large solar storms, high energy particles are hurled with enormous force toward Earth by the Sun. As these particles collide with atmospheric constituents (such as oxygen or nitrogen) unique nuclides of cosmogenic origin are formed in the higher atmosphere. From there they are transported downwards and finally precipitate at the surface due to different sink processes. Their imprints can be conserved over thousands of years within natural archives, such as ice cores or tree rings. Analysis of these natural archives around the globe indicates that the strongest solar storm over the last 10.000 years happened around 774 CE/775. This event is estimated to have been up to two orders of magnitude stronger, than the strongest known events documented for the satellite era. In this study, we model and analyze the transport and deposition of the cosmogenic nuclides produced by the extreme 774 CE/775 event, by applying a new experimental setup. Our results might help to interpret the fingerprints of historical extreme events with respect to the prevailing atmospheric conditions.Key Points: The modeled transport and deposition of the cosmogenic nuclide10Be produced by the 774/775 solar proton event was compared to 10Be ice core records. Hemispheric differences in stratospheric and cross‐tropopause transport, and deposition were identified, with polar summertime maxima of 10Be surface flux. Differences in reconstructed10Be surface fluxes are explained by the local ratio of wet to dry deposition maximizing in the summertime.MEXT Japan Society for the Promotion of Science http://dx.doi.org/10.13039/50110000169